Protein Sequence Retrieval and Phylogenetic Analysis of Various α-amylase Producing Bacillus species

Due to advancements made in biotechnology, genetic manipulation and medium optimization has been possible and that has led to high yield in enzyme especially α-amylase with improvised properties that find its application suitably in detergent, laundry and starch processing industries. Various specie...

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Bibliographic Details
Main Authors: Sonali Priyadarshini, Sukanta Kumar Pradhan, Pratima Ray
Format: Article
Language:English
Published: Bulgarian Academy of Sciences 2020-09-01
Series:International Journal Bioautomation
Subjects:
Online Access:http://www.biomed.bas.bg/bioautomation/2020/vol_24.3/files/24.3_05.pdf
Description
Summary:Due to advancements made in biotechnology, genetic manipulation and medium optimization has been possible and that has led to high yield in enzyme especially α-amylase with improvised properties that find its application suitably in detergent, laundry and starch processing industries. Various species of the genus Bacillus produce α amylases with varying properties. The amylases that find application miraculously in different fields can be easily obtained from plant, animal and microbial sources. Out of the three sources, microbial source especially the genus Bacillus is extensively studied for its ability to produce the enzyme. In the present study, protein sequences of primarily identified α-amylase producing Bacillus spp. were retrieved from protein database of National Center for Biotechnology Information. Multiple sequence alignment was performed and construction of Phylogenetic tree among these sequences was carried out using Neighbor-joining method; data sets based on 1000 re-samplings in molecular genetics evolutionary analysis.
ISSN:1314-1902
1314-2321