In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin
<i>Background and Objectives</i>: Metformin is currently the leading drug of choice for treating type 2 diabetes mellitus, being one of the most widely used drugs worldwide. The beneficial effects of Metformin, however, extend far beyond the reduction of blood glucose. Therefore, this st...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/58/6/705 |
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| author | Gabriel Veniamin Cozma Alexandru Apostu Ioana Macasoi Cristina Adriana Dehelean Octavian Marius Cretu Stefania Dinu Dan Gaiță Aniko Manea |
| author_facet | Gabriel Veniamin Cozma Alexandru Apostu Ioana Macasoi Cristina Adriana Dehelean Octavian Marius Cretu Stefania Dinu Dan Gaiță Aniko Manea |
| author_sort | Gabriel Veniamin Cozma |
| collection | DOAJ |
| description | <i>Background and Objectives</i>: Metformin is currently the leading drug of choice for treating type 2 diabetes mellitus, being one of the most widely used drugs worldwide. The beneficial effects of Metformin, however, extend far beyond the reduction of blood glucose. Therefore, this study aimed to evaluate Metformin’s effects both in vitro and in ovo. <i>Materials and Methods:</i> Metformin has been tested in five different concentrations in human hepatocytes —HepaRG, in terms of cell viability, morphology, structure and number of nuclei and mitochondria, as well as the effect on cell migration. Through the application of HET-CAM, the biocompatibility and potential anti-irritant, as well as protective effects on the vascular plexus were also assessed. <i>Results:</i> According to the results obtained, Metformin increases cell viability without causing morphological changes to cells, mitochondria, or nuclei. Metformin displayed an anti-irritant activity rather than causing irritation at the level of the vascular plexus. <i>Conclusions:</i> In conclusion, Metformin enhances cell viability and proliferation and, has a protective effect on the vascular plexus. Nonetheless, more studies are required to clarify the mechanism of hepatoprotective effect of metformin. |
| first_indexed | 2024-03-09T23:07:00Z |
| format | Article |
| id | doaj.art-875adc4cb3c04bc49dc36f065910bde0 |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-09T23:07:00Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-875adc4cb3c04bc49dc36f065910bde02023-11-23T17:51:06ZengMDPI AGMedicina1010-660X1648-91442022-05-0158670510.3390/medicina58060705In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of MetforminGabriel Veniamin Cozma0Alexandru Apostu1Ioana Macasoi2Cristina Adriana Dehelean3Octavian Marius Cretu4Stefania Dinu5Dan Gaiță6Aniko Manea7Department of Surgical Semiology, Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Cardiology, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 49 No., C. D. Loga Bv., 300041 Timişoara, RomaniaDepartament of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartament of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Surgery, Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Pedodontics, Faculty of Dental Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, RomaniaDepartment of Cardiology, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 49 No., C. D. Loga Bv., 300041 Timişoara, RomaniaDepartment of Obstetrics and Gynecology, Faculty of Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania<i>Background and Objectives</i>: Metformin is currently the leading drug of choice for treating type 2 diabetes mellitus, being one of the most widely used drugs worldwide. The beneficial effects of Metformin, however, extend far beyond the reduction of blood glucose. Therefore, this study aimed to evaluate Metformin’s effects both in vitro and in ovo. <i>Materials and Methods:</i> Metformin has been tested in five different concentrations in human hepatocytes —HepaRG, in terms of cell viability, morphology, structure and number of nuclei and mitochondria, as well as the effect on cell migration. Through the application of HET-CAM, the biocompatibility and potential anti-irritant, as well as protective effects on the vascular plexus were also assessed. <i>Results:</i> According to the results obtained, Metformin increases cell viability without causing morphological changes to cells, mitochondria, or nuclei. Metformin displayed an anti-irritant activity rather than causing irritation at the level of the vascular plexus. <i>Conclusions:</i> In conclusion, Metformin enhances cell viability and proliferation and, has a protective effect on the vascular plexus. Nonetheless, more studies are required to clarify the mechanism of hepatoprotective effect of metformin.https://www.mdpi.com/1648-9144/58/6/705metforminhepatoprotectiveanti-irritantchorioallantoic membraneHET-CAM |
| spellingShingle | Gabriel Veniamin Cozma Alexandru Apostu Ioana Macasoi Cristina Adriana Dehelean Octavian Marius Cretu Stefania Dinu Dan Gaiță Aniko Manea In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin Medicina metformin hepatoprotective anti-irritant chorioallantoic membrane HET-CAM |
| title | In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin |
| title_full | In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin |
| title_fullStr | In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin |
| title_full_unstemmed | In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin |
| title_short | In Vitro and In Ovo Evaluation of the Potential Hepatoprotective Effect of Metformin |
| title_sort | in vitro and in ovo evaluation of the potential hepatoprotective effect of metformin |
| topic | metformin hepatoprotective anti-irritant chorioallantoic membrane HET-CAM |
| url | https://www.mdpi.com/1648-9144/58/6/705 |
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