Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell Culture
The production method of nanoscale detonation carbon (NDC) has recently been developed at Lavrentyev Institute of Hydrodynamics SB RAS. This method uses the reaction of acetylene with oxygen conducted in the detonation mode in fuel-rich acetylene–oxygen mixtures. The morphology and structural featur...
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MDPI AG
2022-07-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/8/1187 |
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| author | Anastasia A. Malakhova Denis K. Rybin Alexandr A. Shtertser Dina V. Dudina |
| author_facet | Anastasia A. Malakhova Denis K. Rybin Alexandr A. Shtertser Dina V. Dudina |
| author_sort | Anastasia A. Malakhova |
| collection | DOAJ |
| description | The production method of nanoscale detonation carbon (NDC) has recently been developed at Lavrentyev Institute of Hydrodynamics SB RAS. This method uses the reaction of acetylene with oxygen conducted in the detonation mode in fuel-rich acetylene–oxygen mixtures. The morphology and structural features of the NDC particles can be varied by changing the concentration of oxygen in the gaseous mixtures. The particles of NDC can serve as reinforcements in metal matrix composites and additives imparting electrical conductivity to polymer matrix composites. Before NDC can be considered for industrial applications, it is necessary to address the related biological safety concerns. The present work was aimed at determining the cytotoxicity of NDC. The NDC powders with two morphologies (obtained using different acetylene/oxygen ratios) were tested on HEK293A human cells. The NDC powder was added to the culture medium in concentrations ranging from 10 ng/mL to 400 μg/mL. The cell viability was determined by a colorimetric EZ4U test and a real-time cell analyzer xCELLigence. None of the NDC samples showed a cytotoxic effect. The results of this study allow us to recommend NDC as a safe and useful product for the development of advanced carbon-based and composite materials. |
| first_indexed | 2024-03-09T12:56:02Z |
| format | Article |
| id | doaj.art-875f5e5160474a259e7da9aab172b34f |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T12:56:02Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-875f5e5160474a259e7da9aab172b34f2023-11-30T21:59:52ZengMDPI AGMicromachines2072-666X2022-07-01138118710.3390/mi13081187Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell CultureAnastasia A. Malakhova0Denis K. Rybin1Alexandr A. Shtertser2Dina V. Dudina3Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentyev Ave. 10, Novosibirsk 630090, RussiaLavrentyev Institute of Hydrodynamics, Siberian Branch of the Russian Academy of Sciences, Lavrentyev Ave. 15, Novosibirsk 630090, RussiaLavrentyev Institute of Hydrodynamics, Siberian Branch of the Russian Academy of Sciences, Lavrentyev Ave. 15, Novosibirsk 630090, RussiaLavrentyev Institute of Hydrodynamics, Siberian Branch of the Russian Academy of Sciences, Lavrentyev Ave. 15, Novosibirsk 630090, RussiaThe production method of nanoscale detonation carbon (NDC) has recently been developed at Lavrentyev Institute of Hydrodynamics SB RAS. This method uses the reaction of acetylene with oxygen conducted in the detonation mode in fuel-rich acetylene–oxygen mixtures. The morphology and structural features of the NDC particles can be varied by changing the concentration of oxygen in the gaseous mixtures. The particles of NDC can serve as reinforcements in metal matrix composites and additives imparting electrical conductivity to polymer matrix composites. Before NDC can be considered for industrial applications, it is necessary to address the related biological safety concerns. The present work was aimed at determining the cytotoxicity of NDC. The NDC powders with two morphologies (obtained using different acetylene/oxygen ratios) were tested on HEK293A human cells. The NDC powder was added to the culture medium in concentrations ranging from 10 ng/mL to 400 μg/mL. The cell viability was determined by a colorimetric EZ4U test and a real-time cell analyzer xCELLigence. None of the NDC samples showed a cytotoxic effect. The results of this study allow us to recommend NDC as a safe and useful product for the development of advanced carbon-based and composite materials.https://www.mdpi.com/2072-666X/13/8/1187cytotoxicitynanoscale detonation carbonhuman cell culture |
| spellingShingle | Anastasia A. Malakhova Denis K. Rybin Alexandr A. Shtertser Dina V. Dudina Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell Culture Micromachines cytotoxicity nanoscale detonation carbon human cell culture |
| title | Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell Culture |
| title_full | Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell Culture |
| title_fullStr | Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell Culture |
| title_full_unstemmed | Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell Culture |
| title_short | Nanoscale Detonation Carbon Demonstrates Biosafety in Human Cell Culture |
| title_sort | nanoscale detonation carbon demonstrates biosafety in human cell culture |
| topic | cytotoxicity nanoscale detonation carbon human cell culture |
| url | https://www.mdpi.com/2072-666X/13/8/1187 |
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