Chemical Profile and Antioxidant and Antimicrobial Activity of <i>Rosa canina</i> L. Dried Fruit Commercially Available in Serbia
The aim of this work was to give as much information as possible on <i>Rosa canina</i> dried fruit that is commercially available in Serbia. In order to provide the chemical composition, the UHPLC-DAD-ESI-MS method was employed for both polar and non-polar extracts of samples obtained wi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/25/5/2518 |
| Summary: | The aim of this work was to give as much information as possible on <i>Rosa canina</i> dried fruit that is commercially available in Serbia. In order to provide the chemical composition, the UHPLC-DAD-ESI-MS method was employed for both polar and non-polar extracts of samples obtained with a solvent mixture consisting of hexane, acetone, and ethanol in a volume ratio of 2:1:1, respectively, and 0.05% (<i>w</i>/<i>v</i>) butylated hydroxytoluene. In addition, the total content levels of lycopene, <i>β</i>-carotene, total polyphenols, and flavonoids were determined by means of UV-vis spectrophotometry. The antioxidant activity was tested by applying four different methods: ABTS, DPPH, FRAP, and CUPRAC. Overall, nine compounds were identified. The results of chemical composition analysis were used as the basis for the interpretation of the calculated results for the antioxidant and antimicrobial activity. The obtained results for <i>R. canina</i> dried fruit extract are as follows: <i>β</i>-carotene—7.25 [mg/100 g fruit weight]; lycopene—2.34 (mg/100 g FW); total polyphenol content (TPC)—2980 [mg GAE/kg FW]; total flavonoid content (TFC)—1454 [mg CE/kg FW]; antioxidant activity—ABTS 12.3 [μmol/100 g FW], DPPH 6.84, FRAP 52.04, and CUPRAC 15,425; and antimicrobial activity—<i>Staphylococcus aureus</i> MIC/MMC 4/0 [mg∙mL<sup>−1</sup>], <i>Enterococcus faecalis</i> 4/0, <i>Bacillus cereus</i> 4/0, <i>Escherichia coli</i> 4/0, <i>Salmonella enteritidis</i> 4/4, <i>Enteroabacter aerogenes</i> 4/0, <i>Pseudomonas aeruginosa</i> 2/0, and <i>Candida albicans</i> 2/0. |
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| ISSN: | 1661-6596 1422-0067 |