Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
Summary: Glycan-glycan interactions between viral particles and host cells may lengthen the dwell time of the virus on the cell surface to facilitate cellular receptor engagement. Here, we present a protocol for visualizing glycan-mediated binding between virus or virus-like-particles (VLPs) and hum...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723006408 |
| _version_ | 1827781684649525248 |
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| author | Belinda L. Spillings Kathryn Green Erica Lovas Richard Webb Johnson Mak |
| author_facet | Belinda L. Spillings Kathryn Green Erica Lovas Richard Webb Johnson Mak |
| author_sort | Belinda L. Spillings |
| collection | DOAJ |
| description | Summary: Glycan-glycan interactions between viral particles and host cells may lengthen the dwell time of the virus on the cell surface to facilitate cellular receptor engagement. Here, we present a protocol for visualizing glycan-mediated binding between virus or virus-like-particles (VLPs) and human peripheral blood mononuclear cells using transmission electron microscopy (TEM). We describe steps for virus and VLP production, isolation of human peripheral blood mononuclear cells, and sample preparation. We then detail procedures for thin-section TEM.For complete details on the use and execution of this protocol, please refer to Spillings et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-11T15:22:10Z |
| format | Article |
| id | doaj.art-8764e1de67ea474db4c15c1b4b64de59 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-11T15:22:10Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-8764e1de67ea474db4c15c1b4b64de592023-10-28T05:09:41ZengElsevierSTAR Protocols2666-16672023-12-0144102673Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopyBelinda L. Spillings0Kathryn Green1Erica Lovas2Richard Webb3Johnson Mak4Institute for Glycomics, Griffith University, Gold Coast, QLD 4222, Australia; Corresponding authorCentre for Microscopy and Microanalysis, The University of Queensland, Brisbane, QLD 4072, AustraliaCentre for Microscopy and Microanalysis, The University of Queensland, Brisbane, QLD 4072, AustraliaCentre for Microscopy and Microanalysis, The University of Queensland, Brisbane, QLD 4072, AustraliaInstitute for Glycomics, Griffith University, Gold Coast, QLD 4222, Australia; Corresponding authorSummary: Glycan-glycan interactions between viral particles and host cells may lengthen the dwell time of the virus on the cell surface to facilitate cellular receptor engagement. Here, we present a protocol for visualizing glycan-mediated binding between virus or virus-like-particles (VLPs) and human peripheral blood mononuclear cells using transmission electron microscopy (TEM). We describe steps for virus and VLP production, isolation of human peripheral blood mononuclear cells, and sample preparation. We then detail procedures for thin-section TEM.For complete details on the use and execution of this protocol, please refer to Spillings et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723006408Cell BiologyMicrobiologyMicroscopy |
| spellingShingle | Belinda L. Spillings Kathryn Green Erica Lovas Richard Webb Johnson Mak Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy STAR Protocols Cell Biology Microbiology Microscopy |
| title | Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy |
| title_full | Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy |
| title_fullStr | Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy |
| title_full_unstemmed | Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy |
| title_short | Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy |
| title_sort | protocol to visualize glycan mediated binding between virus and human blood mononuclear cells using thin section transmission electron microscopy |
| topic | Cell Biology Microbiology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723006408 |
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