Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides
Abstract Background Streptomycetes from the rhizospheric soils are a rich resource of novel secondary metabolites with various biological activities. However, there is still little information related to the isolation, antimicrobial activity and biosynthetic potential for polyketide and non-ribosoma...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-06-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-020-01832-5 |
| _version_ | 1819060261575720960 |
|---|---|
| author | Fei Peng Meng-Yue Zhang Shao-Yang Hou Juan Chen Ying-Ying Wu Yi-Xuan Zhang |
| author_facet | Fei Peng Meng-Yue Zhang Shao-Yang Hou Juan Chen Ying-Ying Wu Yi-Xuan Zhang |
| author_sort | Fei Peng |
| collection | DOAJ |
| description | Abstract Background Streptomycetes from the rhizospheric soils are a rich resource of novel secondary metabolites with various biological activities. However, there is still little information related to the isolation, antimicrobial activity and biosynthetic potential for polyketide and non-ribosomal peptide discovery associated with the rhizospheric streptomycetes of Panax notoginseng. Thus, the aims of the present study are to (i) identify culturable streptomycetes from the rhizospheric soil of P. notoginseng by 16S rRNA gene, (ii) evaluate the antimicrobial activities of isolates and analyze the biosynthetic gene encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) of isolates, (iii) detect the bioactive secondary metabolites from selected streptomycetes, (iv) study the influence of the selected isolate on the growth of P. notoginseng in the continuous cropping field. This study would provide a preliminary basis for the further discovery of the secondary metabolites from streptomycetes isolated from the rhizospheric soil of P. notoginseng and their further utilization for biocontrol of plants. Results A total of 42 strains representing 42 species of the genus Streptomyces were isolated from 12 rhizospheric soil samples in the cultivation field of P. notoginseng and were analyzed by 16S rRNA gene sequencing. Overall, 40 crude cell extracts out of 42 under two culture conditions showed antibacterial and antifungal activities. Also, the presence of biosynthesis genes encoding type I and II polyketide synthase (PKS I and PKS II) and nonribosomal peptide synthetases (NRPSs) in 42 strains were established. Based on characteristic chemical profiles screening by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD), the secondary metabolite profiles of strain SYP-A7257 were evaluated by High Performance Liquid Chromatography-High Resolution Mass Spectrometry (HPLC-HRMS). Finally, four compounds actinomycin X2 (F1), fungichromin (F2), thailandin B (F7) and antifungalmycin (F8) were isolated from strain SYP-A7257 by using chromatography techniques, UV, HR-ESI-MS and NMR, and their antimicrobial activities against the test bacteria and fungus were also evaluated. In the farm experiments, Streptomyces sp. SYP-A7257 showed healthy growth promotion and survival rate improvement of P. notoginseng in the continuous cropping field. Conclusions We demonstrated the P. notoginseng rhizospheric soil-derived Streptomyces spp. distribution and diversity with respect to their metabolic potential for polyketides and non-ribosomal peptides, as well as the presence of biosynthesis genes PKS I, PKS II and NRPSs. Our results showed that cultivatable Streptomyces isolates from the rhizospheric soils of P. notoginseng have the ability to produce bioactive secondary metabolites. The farm experiments suggested that the rhizospheric soil Streptomyces sp. SYP-A7257 may be a potential biological control agent for healthy growth promotion and survival rate improvement of P. notoginseng in the continuous cropping field. |
| first_indexed | 2024-12-21T14:24:11Z |
| format | Article |
| id | doaj.art-87656fec8e2b4a4d995ffac9892b14a6 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-21T14:24:11Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-87656fec8e2b4a4d995ffac9892b14a62022-12-21T19:00:42ZengBMCBMC Microbiology1471-21802020-06-0120111610.1186/s12866-020-01832-5Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptidesFei Peng0Meng-Yue Zhang1Shao-Yang Hou2Juan Chen3Ying-Ying Wu4Yi-Xuan Zhang5School of Life Science and Biopharmaceutics, Shenyang Pharmaceutical UniversitySchool of Life Science and Biopharmaceutics, Shenyang Pharmaceutical UniversitySchool of Life Science and Biopharmaceutics, Shenyang Pharmaceutical UniversitySchool of Life Science and Biopharmaceutics, Shenyang Pharmaceutical UniversitySchool of Life Science and Biopharmaceutics, Shenyang Pharmaceutical UniversitySchool of Life Science and Biopharmaceutics, Shenyang Pharmaceutical UniversityAbstract Background Streptomycetes from the rhizospheric soils are a rich resource of novel secondary metabolites with various biological activities. However, there is still little information related to the isolation, antimicrobial activity and biosynthetic potential for polyketide and non-ribosomal peptide discovery associated with the rhizospheric streptomycetes of Panax notoginseng. Thus, the aims of the present study are to (i) identify culturable streptomycetes from the rhizospheric soil of P. notoginseng by 16S rRNA gene, (ii) evaluate the antimicrobial activities of isolates and analyze the biosynthetic gene encoding polyketide synthases (PKSs) and nonribosomal peptide synthetases (NRPSs) of isolates, (iii) detect the bioactive secondary metabolites from selected streptomycetes, (iv) study the influence of the selected isolate on the growth of P. notoginseng in the continuous cropping field. This study would provide a preliminary basis for the further discovery of the secondary metabolites from streptomycetes isolated from the rhizospheric soil of P. notoginseng and their further utilization for biocontrol of plants. Results A total of 42 strains representing 42 species of the genus Streptomyces were isolated from 12 rhizospheric soil samples in the cultivation field of P. notoginseng and were analyzed by 16S rRNA gene sequencing. Overall, 40 crude cell extracts out of 42 under two culture conditions showed antibacterial and antifungal activities. Also, the presence of biosynthesis genes encoding type I and II polyketide synthase (PKS I and PKS II) and nonribosomal peptide synthetases (NRPSs) in 42 strains were established. Based on characteristic chemical profiles screening by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD), the secondary metabolite profiles of strain SYP-A7257 were evaluated by High Performance Liquid Chromatography-High Resolution Mass Spectrometry (HPLC-HRMS). Finally, four compounds actinomycin X2 (F1), fungichromin (F2), thailandin B (F7) and antifungalmycin (F8) were isolated from strain SYP-A7257 by using chromatography techniques, UV, HR-ESI-MS and NMR, and their antimicrobial activities against the test bacteria and fungus were also evaluated. In the farm experiments, Streptomyces sp. SYP-A7257 showed healthy growth promotion and survival rate improvement of P. notoginseng in the continuous cropping field. Conclusions We demonstrated the P. notoginseng rhizospheric soil-derived Streptomyces spp. distribution and diversity with respect to their metabolic potential for polyketides and non-ribosomal peptides, as well as the presence of biosynthesis genes PKS I, PKS II and NRPSs. Our results showed that cultivatable Streptomyces isolates from the rhizospheric soils of P. notoginseng have the ability to produce bioactive secondary metabolites. The farm experiments suggested that the rhizospheric soil Streptomyces sp. SYP-A7257 may be a potential biological control agent for healthy growth promotion and survival rate improvement of P. notoginseng in the continuous cropping field.http://link.springer.com/article/10.1186/s12866-020-01832-5StreptomycesPanax notoginsengPhylogenetic analysisType I polyketide synthases (PKS I)Type II polyketide synthase (PKS II)Nonribosomal peptide synthetases (NRPS) |
| spellingShingle | Fei Peng Meng-Yue Zhang Shao-Yang Hou Juan Chen Ying-Ying Wu Yi-Xuan Zhang Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides BMC Microbiology Streptomyces Panax notoginseng Phylogenetic analysis Type I polyketide synthases (PKS I) Type II polyketide synthase (PKS II) Nonribosomal peptide synthetases (NRPS) |
| title | Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides |
| title_full | Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides |
| title_fullStr | Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides |
| title_full_unstemmed | Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides |
| title_short | Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides |
| title_sort | insights into streptomyces spp isolated from the rhizospheric soil of panax notoginseng isolation antimicrobial activity and biosynthetic potential for polyketides and non ribosomal peptides |
| topic | Streptomyces Panax notoginseng Phylogenetic analysis Type I polyketide synthases (PKS I) Type II polyketide synthase (PKS II) Nonribosomal peptide synthetases (NRPS) |
| url | http://link.springer.com/article/10.1186/s12866-020-01832-5 |
| work_keys_str_mv | AT feipeng insightsintostreptomycessppisolatedfromtherhizosphericsoilofpanaxnotoginsengisolationantimicrobialactivityandbiosyntheticpotentialforpolyketidesandnonribosomalpeptides AT mengyuezhang insightsintostreptomycessppisolatedfromtherhizosphericsoilofpanaxnotoginsengisolationantimicrobialactivityandbiosyntheticpotentialforpolyketidesandnonribosomalpeptides AT shaoyanghou insightsintostreptomycessppisolatedfromtherhizosphericsoilofpanaxnotoginsengisolationantimicrobialactivityandbiosyntheticpotentialforpolyketidesandnonribosomalpeptides AT juanchen insightsintostreptomycessppisolatedfromtherhizosphericsoilofpanaxnotoginsengisolationantimicrobialactivityandbiosyntheticpotentialforpolyketidesandnonribosomalpeptides AT yingyingwu insightsintostreptomycessppisolatedfromtherhizosphericsoilofpanaxnotoginsengisolationantimicrobialactivityandbiosyntheticpotentialforpolyketidesandnonribosomalpeptides AT yixuanzhang insightsintostreptomycessppisolatedfromtherhizosphericsoilofpanaxnotoginsengisolationantimicrobialactivityandbiosyntheticpotentialforpolyketidesandnonribosomalpeptides |