<i>In Silico</i> and Validation Approaches for Optimum Conditions of <i>Rattus norvegicus</i> Target Gene qPCR Primers

<i>In Silico</i> and Validation Approaches for Optimum Conditions of <i>Rattus norvegicus</i> Target Gene qPCR Primers

The qPCR method requires an oligonucleotide pair to prime the amplification process. With the variety of qPCR reagent and primer options available, in silico and laboratory experimental validation approach was needed to validate the most suitable primer for prior use. This article aims to provide in...

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Bibliographic Details
Main Authors: Gracia Alice Victoria Pollo, Nyoman Yudi Antara, Firman Alamsyah, Rarastoeti Pratiwi
Format: Article
Language:English
Published: Universitas Gadjah Mada 2023-06-01
Series:Journal of Tropical Biodiversity and Biotechnology
Subjects:
qpcr primer
in silico
experimental validation
Online Access:https://journal.ugm.ac.id/jtbb/article/view/71765

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