Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) Fruit
A physiological disease of the pineapple fruit called pineapple translucency causes the pulp to become water-soaked, which affects the fruit’s taste, flavor, shelf life, and integrity. In the present study, we analyzed seven pineapple varieties, of which three were watery and four were non-watery. T...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/8/7199 |
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| author | Jing Chen Yanli Yao Hui Zeng Xiumei Zhang |
| author_facet | Jing Chen Yanli Yao Hui Zeng Xiumei Zhang |
| author_sort | Jing Chen |
| collection | DOAJ |
| description | A physiological disease of the pineapple fruit called pineapple translucency causes the pulp to become water-soaked, which affects the fruit’s taste, flavor, shelf life, and integrity. In the present study, we analyzed seven pineapple varieties, of which three were watery and four were non-watery. There were no apparent macronutritional (K, P, or N) differences in their pulp, but the non-watery pineapple varieties had higher dry matter and soluble sugar content. The metabolomic analysis found 641 metabolites and revealed differential expression of alkaloids, phenolic acids, nucleotide derivatives, lipids, and other metabolites among the seven species. Transcriptome analysis and further KEGG enrichment showed downregulation of ‘flavonoid biosynthesis’ pathways, differential expression of metabolic pathways, secondary metabolites biosynthesis, plant–pathogen interaction, and plant hormone signal transduction. We believe this study will provide critical molecular data supporting a deeper understanding of pineapple translucency formation and greatly benefit future research on this commercially important crop. |
| first_indexed | 2024-03-11T04:57:16Z |
| format | Article |
| id | doaj.art-87666aacaab145c19efdfc21feb331b2 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:57:16Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-87666aacaab145c19efdfc21feb331b22023-11-17T19:36:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248719910.3390/ijms24087199Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) FruitJing Chen0Yanli Yao1Hui Zeng2Xiumei Zhang3The South Subtropical Crops Research Institute of Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaThe South Subtropical Crops Research Institute of Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaThe South Subtropical Crops Research Institute of Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaThe South Subtropical Crops Research Institute of Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, ChinaA physiological disease of the pineapple fruit called pineapple translucency causes the pulp to become water-soaked, which affects the fruit’s taste, flavor, shelf life, and integrity. In the present study, we analyzed seven pineapple varieties, of which three were watery and four were non-watery. There were no apparent macronutritional (K, P, or N) differences in their pulp, but the non-watery pineapple varieties had higher dry matter and soluble sugar content. The metabolomic analysis found 641 metabolites and revealed differential expression of alkaloids, phenolic acids, nucleotide derivatives, lipids, and other metabolites among the seven species. Transcriptome analysis and further KEGG enrichment showed downregulation of ‘flavonoid biosynthesis’ pathways, differential expression of metabolic pathways, secondary metabolites biosynthesis, plant–pathogen interaction, and plant hormone signal transduction. We believe this study will provide critical molecular data supporting a deeper understanding of pineapple translucency formation and greatly benefit future research on this commercially important crop.https://www.mdpi.com/1422-0067/24/8/7199pineappletranslucencyflavonoidtranscriptomemetabolome |
| spellingShingle | Jing Chen Yanli Yao Hui Zeng Xiumei Zhang Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) Fruit International Journal of Molecular Sciences pineapple translucency flavonoid transcriptome metabolome |
| title | Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) Fruit |
| title_full | Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) Fruit |
| title_fullStr | Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) Fruit |
| title_full_unstemmed | Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) Fruit |
| title_short | Integrated Metabolome and Transcriptome Analysis Reveals a Potential Mechanism for Water Accumulation Mediated Translucency in Pineapple (<i>Ananas comosus</i> (L.) Merr.) Fruit |
| title_sort | integrated metabolome and transcriptome analysis reveals a potential mechanism for water accumulation mediated translucency in pineapple i ananas comosus i l merr fruit |
| topic | pineapple translucency flavonoid transcriptome metabolome |
| url | https://www.mdpi.com/1422-0067/24/8/7199 |
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