A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in Kazakhstan
Four aerobic bacteria with bacteriolytic capabilities were isolated from the brackish water site Strait Uzynaral of Lake Balkhash in Kazakhstan. The morphology and physiology of the bacterial isolates have subsequently been analyzed. Using matrix assisted laser desorption ionization-time of flight m...
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2022-07-01
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author | Alexander Meene Christiane Herzer Rabea Schlüter Bolatkhan Zayadan Ruediger Pukall Peter Schumann Frieder Schauer Tim Urich Annett Mikolasch |
author_facet | Alexander Meene Christiane Herzer Rabea Schlüter Bolatkhan Zayadan Ruediger Pukall Peter Schumann Frieder Schauer Tim Urich Annett Mikolasch |
author_sort | Alexander Meene |
collection | DOAJ |
description | Four aerobic bacteria with bacteriolytic capabilities were isolated from the brackish water site Strait Uzynaral of Lake Balkhash in Kazakhstan. The morphology and physiology of the bacterial isolates have subsequently been analyzed. Using matrix assisted laser desorption ionization-time of flight mass spectrum and partial 16S rRNA gene sequence analyses, three of the isolates have been identified as <i>Pseudomonas veronii</i> and one as <i>Paenibacillus apiarius</i>. We determined the capability of both species to lyse pre-grown cells of the Gram-negative strains <i>Pseudomonas putida</i> SBUG 24 and <i>Escherichia coli</i> SBUG 13 as well as the Gram-positive strains <i>Micrococcus luteus</i> SBUG 16 and <i>Arthrobacter citreus</i> SBUG 321 on solid media. The bacteriolysis process was analyzed by creating growth curves and electron micrographs of co-cultures with the bacteriolytic isolates and the lysis sensitive strain <i>Arthrobacter citreus</i> SBUG 321 in nutrient-poor liquid media. One metabolite of <i>Paenibacillus apiarius</i> was isolated and structurally characterized by various chemical structure determination methods. It is a novel antibiotic substance. |
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language | English |
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spelling | doaj.art-8768b68e11864537a24188271b15ce5f2023-12-03T14:08:56ZengMDPI AGMicroorganisms2076-26072022-07-01108151910.3390/microorganisms10081519A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in KazakhstanAlexander Meene0Christiane Herzer1Rabea Schlüter2Bolatkhan Zayadan3Ruediger Pukall4Peter Schumann5Frieder Schauer6Tim Urich7Annett Mikolasch8Institute of Microbiology, University Greifswald, Felix-Hausdorff-Straße 8, 17487 Greifswald, GermanyInstitute of Microbiology, University Greifswald, Felix-Hausdorff-Straße 8, 17487 Greifswald, GermanyImaging Center of the Department of Biology, University Greifswald, Friedrich-Ludwig-Jahn-Str. 15, 17487 Greifswald, GermanyDepartment of Biotechnology, Al-Farabi Kazakh National University, 71, al-Farabi Ave, Almaty 050040, KazakhstanLeibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124 Braunschweig, GermanyLeibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7B, 38124 Braunschweig, GermanyInstitute of Microbiology, University Greifswald, Felix-Hausdorff-Straße 8, 17487 Greifswald, GermanyInstitute of Microbiology, University Greifswald, Felix-Hausdorff-Straße 8, 17487 Greifswald, GermanyInstitute of Microbiology, University Greifswald, Felix-Hausdorff-Straße 8, 17487 Greifswald, GermanyFour aerobic bacteria with bacteriolytic capabilities were isolated from the brackish water site Strait Uzynaral of Lake Balkhash in Kazakhstan. The morphology and physiology of the bacterial isolates have subsequently been analyzed. Using matrix assisted laser desorption ionization-time of flight mass spectrum and partial 16S rRNA gene sequence analyses, three of the isolates have been identified as <i>Pseudomonas veronii</i> and one as <i>Paenibacillus apiarius</i>. We determined the capability of both species to lyse pre-grown cells of the Gram-negative strains <i>Pseudomonas putida</i> SBUG 24 and <i>Escherichia coli</i> SBUG 13 as well as the Gram-positive strains <i>Micrococcus luteus</i> SBUG 16 and <i>Arthrobacter citreus</i> SBUG 321 on solid media. The bacteriolysis process was analyzed by creating growth curves and electron micrographs of co-cultures with the bacteriolytic isolates and the lysis sensitive strain <i>Arthrobacter citreus</i> SBUG 321 in nutrient-poor liquid media. One metabolite of <i>Paenibacillus apiarius</i> was isolated and structurally characterized by various chemical structure determination methods. It is a novel antibiotic substance.https://www.mdpi.com/2076-2607/10/8/1519predationbacterivorylipopeptides<i>Micrococcus luteus</i><i>Pseudomonas putida</i><i>Escherichia coli</i> |
spellingShingle | Alexander Meene Christiane Herzer Rabea Schlüter Bolatkhan Zayadan Ruediger Pukall Peter Schumann Frieder Schauer Tim Urich Annett Mikolasch A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in Kazakhstan Microorganisms predation bacterivory lipopeptides <i>Micrococcus luteus</i> <i>Pseudomonas putida</i> <i>Escherichia coli</i> |
title | A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in Kazakhstan |
title_full | A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in Kazakhstan |
title_fullStr | A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in Kazakhstan |
title_full_unstemmed | A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in Kazakhstan |
title_short | A Novel Antimicrobial Metabolite Produced by <i>Paenibacillus apiarius</i> Isolated from Brackish Water of Lake Balkhash in Kazakhstan |
title_sort | novel antimicrobial metabolite produced by i paenibacillus apiarius i isolated from brackish water of lake balkhash in kazakhstan |
topic | predation bacterivory lipopeptides <i>Micrococcus luteus</i> <i>Pseudomonas putida</i> <i>Escherichia coli</i> |
url | https://www.mdpi.com/2076-2607/10/8/1519 |
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