Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation.
Gut dysbiosis has been associated with worse allogeneic hematopoietic cell transplantation (allo-HCT) outcomes. We reported an association between intrinsically vancomycin-resistant enterococci (iVRE: E. gallinarum and E. casseliflavus) gut colonization and lower post-transplant mortality. In this s...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0220850 |
| _version_ | 1819122790267092992 |
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| author | Armin Rashidi Maryam Ebadi Robin R Shields-Cutler Kathryn Kruziki Dawn A Manias Aaron M T Barnes Todd E DeFor Patricia Ferrieri Jo-Anne H Young Dan Knights Bruce R Blazar Daniel J Weisdorf Gary M Dunny |
| author_facet | Armin Rashidi Maryam Ebadi Robin R Shields-Cutler Kathryn Kruziki Dawn A Manias Aaron M T Barnes Todd E DeFor Patricia Ferrieri Jo-Anne H Young Dan Knights Bruce R Blazar Daniel J Weisdorf Gary M Dunny |
| author_sort | Armin Rashidi |
| collection | DOAJ |
| description | Gut dysbiosis has been associated with worse allogeneic hematopoietic cell transplantation (allo-HCT) outcomes. We reported an association between intrinsically vancomycin-resistant enterococci (iVRE: E. gallinarum and E. casseliflavus) gut colonization and lower post-transplant mortality. In this study, using an expanded cohort, we evaluated whether our previously observed association is species-specific. We included allo-HCT recipients with ≥1 positive rectal swab or stool culture for iVRE between days -14 and +14 of transplant. To investigate whether iVRE modulate the gut microbiota, we performed agar diffusion assays. To investigate whether iVRE differ in their ability to activate the aryl hydrocarbon receptor, we analyzed iVRE genomes for enzymes in the shikimate and tryptophan pathways. Sixty six (23 E. casseliflavus and 43 E. gallinarum) of the 908 allograft recipients (2011-2017) met our inclusion criteria. Overall survival was significantly higher in patients with E. casseliflavus (91% vs. 62% at 3 years, P = 0.04). In multivariable analysis, E. casseliflavus gut colonization was significantly associated with reduced all-cause mortality (hazard ratio 0.20, 95% confidence interval 0.04-0.91, P = 0.04). While agar assays were largely unremarkable, genome mining predicted that E. casseliflavus encodes a larger number of enzymes in the tryptophan metabolism pathway. In conclusion, E. casseliflavus gut colonization is associated with reduced post-HCT morality. Further research is needed to understand the mechanisms for this association. |
| first_indexed | 2024-12-22T06:58:03Z |
| format | Article |
| id | doaj.art-8768e548f7e84911883974b3cd1b1356 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T06:58:03Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8768e548f7e84911883974b3cd1b13562022-12-21T18:34:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01148e022085010.1371/journal.pone.0220850Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation.Armin RashidiMaryam EbadiRobin R Shields-CutlerKathryn KruzikiDawn A ManiasAaron M T BarnesTodd E DeForPatricia FerrieriJo-Anne H YoungDan KnightsBruce R BlazarDaniel J WeisdorfGary M DunnyGut dysbiosis has been associated with worse allogeneic hematopoietic cell transplantation (allo-HCT) outcomes. We reported an association between intrinsically vancomycin-resistant enterococci (iVRE: E. gallinarum and E. casseliflavus) gut colonization and lower post-transplant mortality. In this study, using an expanded cohort, we evaluated whether our previously observed association is species-specific. We included allo-HCT recipients with ≥1 positive rectal swab or stool culture for iVRE between days -14 and +14 of transplant. To investigate whether iVRE modulate the gut microbiota, we performed agar diffusion assays. To investigate whether iVRE differ in their ability to activate the aryl hydrocarbon receptor, we analyzed iVRE genomes for enzymes in the shikimate and tryptophan pathways. Sixty six (23 E. casseliflavus and 43 E. gallinarum) of the 908 allograft recipients (2011-2017) met our inclusion criteria. Overall survival was significantly higher in patients with E. casseliflavus (91% vs. 62% at 3 years, P = 0.04). In multivariable analysis, E. casseliflavus gut colonization was significantly associated with reduced all-cause mortality (hazard ratio 0.20, 95% confidence interval 0.04-0.91, P = 0.04). While agar assays were largely unremarkable, genome mining predicted that E. casseliflavus encodes a larger number of enzymes in the tryptophan metabolism pathway. In conclusion, E. casseliflavus gut colonization is associated with reduced post-HCT morality. Further research is needed to understand the mechanisms for this association.https://doi.org/10.1371/journal.pone.0220850 |
| spellingShingle | Armin Rashidi Maryam Ebadi Robin R Shields-Cutler Kathryn Kruziki Dawn A Manias Aaron M T Barnes Todd E DeFor Patricia Ferrieri Jo-Anne H Young Dan Knights Bruce R Blazar Daniel J Weisdorf Gary M Dunny Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation. PLoS ONE |
| title | Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation. |
| title_full | Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation. |
| title_fullStr | Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation. |
| title_full_unstemmed | Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation. |
| title_short | Early E. casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation. |
| title_sort | early e casseliflavus gut colonization and outcomes of allogeneic hematopoietic cell transplantation |
| url | https://doi.org/10.1371/journal.pone.0220850 |
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