microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG
ObjectiveSquamous cell carcinoma (SCC) occurring in the tonsils (TSCC) has a poorer prognosis than SCC occurring in other regions of the oral cavity (non-tonsillar SCC [NTSCC]) because it easily metastasizes to distant organs. This study aimed to elucidate the molecular mechanisms underlying the mig...
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1239224/full |
| _version_ | 1797755533090881536 |
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| author | Shunsuke Noguchi Asuka Matsui |
| author_facet | Shunsuke Noguchi Asuka Matsui |
| author_sort | Shunsuke Noguchi |
| collection | DOAJ |
| description | ObjectiveSquamous cell carcinoma (SCC) occurring in the tonsils (TSCC) has a poorer prognosis than SCC occurring in other regions of the oral cavity (non-tonsillar SCC [NTSCC]) because it easily metastasizes to distant organs. This study aimed to elucidate the molecular mechanisms underlying the migration and invasion of TSCC cells in vitro.Materials and methodsThis study focused on differential microRNA (miRNA) expression using microRNA microarrays and quantitative polymerase chain reaction in canine TSCC and NTSCC tissues and cell lines. A target gene of the miRNA involved in cell migration and invasion was validated by wound healing, transwell, and luciferase assays.ResultsmiR-203 expression was lower in TSCC tissues than in the normal oral mucosa and NTSCC tissues. Transfection of the miR-203 mimic resulted in the downregulation of mesenchymal marker protein expression and attenuation of cell migration and invasion in TSCC cells, but not in NTSCC cells. A dual-luciferase assay revealed that miR-203 directly targeted the mesenchymal transcription factor SLUG. SLUG overexpression enhances the migration of TSCC cells.ConclusionOur study indicates that the miR-203/SLUG axis may be involved in the metastatic mechanisms of TSCC. |
| first_indexed | 2024-03-12T17:47:38Z |
| format | Article |
| id | doaj.art-876e1461365f49d393281c0c9078e03b |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-12T17:47:38Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-876e1461365f49d393281c0c9078e03b2023-08-03T12:21:39ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692023-08-011010.3389/fvets.2023.12392241239224microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUGShunsuke Noguchi0Asuka Matsui1Laboratory of Veterinary Radiation, Graduate School of Veterinary Science, Osaka Metropolitan University, Osaka, JapanLaboratory of Veterinary Radiology, College of Life, Environment, and Advanced Sciences, Osaka Metropolitan University, Osaka, JapanObjectiveSquamous cell carcinoma (SCC) occurring in the tonsils (TSCC) has a poorer prognosis than SCC occurring in other regions of the oral cavity (non-tonsillar SCC [NTSCC]) because it easily metastasizes to distant organs. This study aimed to elucidate the molecular mechanisms underlying the migration and invasion of TSCC cells in vitro.Materials and methodsThis study focused on differential microRNA (miRNA) expression using microRNA microarrays and quantitative polymerase chain reaction in canine TSCC and NTSCC tissues and cell lines. A target gene of the miRNA involved in cell migration and invasion was validated by wound healing, transwell, and luciferase assays.ResultsmiR-203 expression was lower in TSCC tissues than in the normal oral mucosa and NTSCC tissues. Transfection of the miR-203 mimic resulted in the downregulation of mesenchymal marker protein expression and attenuation of cell migration and invasion in TSCC cells, but not in NTSCC cells. A dual-luciferase assay revealed that miR-203 directly targeted the mesenchymal transcription factor SLUG. SLUG overexpression enhances the migration of TSCC cells.ConclusionOur study indicates that the miR-203/SLUG axis may be involved in the metastatic mechanisms of TSCC.https://www.frontiersin.org/articles/10.3389/fvets.2023.1239224/fulldogmicroRNA-203migration and invasionSLUGsquamous cell carcinoma |
| spellingShingle | Shunsuke Noguchi Asuka Matsui microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG Frontiers in Veterinary Science dog microRNA-203 migration and invasion SLUG squamous cell carcinoma |
| title | microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG |
| title_full | microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG |
| title_fullStr | microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG |
| title_full_unstemmed | microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG |
| title_short | microRNA-203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting SLUG |
| title_sort | microrna 203 inhibits migration and invasion of canine tonsillar squamous cell carcinoma cells by targeting slug |
| topic | dog microRNA-203 migration and invasion SLUG squamous cell carcinoma |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1239224/full |
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