Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1)
Introduction. Tat protein is a major factor of HIV (human immunodeficiency virus) transcription regulation and has other activities. Tat is characterized by high variability, with some amino acid substitutions, including subtypespecific ones, being able to influence on its functionality. HIV type 1...
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Central Research Institute for Epidemiology
2022-01-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/579/358 |
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| author | A. I. Kuznetsova K. B. Gromov D. E. Kireev A. V. Shlykova A. E. Lopatukhin E. V. Kazennova A. V. Lebedev A. S. Tumanov K. V. Kim M. R. Bobkova |
| author_facet | A. I. Kuznetsova K. B. Gromov D. E. Kireev A. V. Shlykova A. E. Lopatukhin E. V. Kazennova A. V. Lebedev A. S. Tumanov K. V. Kim M. R. Bobkova |
| author_sort | A. I. Kuznetsova |
| collection | DOAJ |
| description | Introduction. Tat protein is a major factor of HIV (human immunodeficiency virus) transcription regulation and has other activities. Tat is characterized by high variability, with some amino acid substitutions, including subtypespecific ones, being able to influence on its functionality. HIV type 1 (HIV-1) sub-subtype A6 is the most widespread in Russia. Previous studies of the polymorphisms in structural regions of the A6 variant have shown numerous characteristic features; however, Tat polymorphism in A6 has not been studied.
Goals and tasks. The main goal of the work was to analyze the characteristics of Tat protein in HIV-1 A6 variant, that is, to identify substitutions characteristic for A6 and A1 variants, as well as to compare the frequency of mutations in functionally significant domains in sub-subtype A6 and subtype B.
Material and methods. The nucleotide sequences of HIV-1 sub-subtypes A6, A1, A2, A3, A4, subtype B and the reference nucleotide sequence were obtained from the Los Alamos international database.
Results and discussion. Q54H and Q60H were identified as characteristic substitutions. Essential differences in natural polymorphisms between sub-subtypes A6 and A1 have been demonstrated. In the CPP-region, there were detected mutations (R53K, Q54H, Q54P, R57G) which were more common in sub-subtype A6 than in subtype B.
Conclusion. Tat protein of sub-subtype A6 have some characteristics that make it possible to reliably distinguish it from other HIV-1 variants. Mutations identified in the CPP region could potentially alter the activity of Tat. The data obtained could form the basis for the drugs and vaccines development. |
| first_indexed | 2024-03-13T00:08:48Z |
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| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:48Z |
| publishDate | 2022-01-01 |
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| series | Вопросы вирусологии |
| spelling | doaj.art-876f65d18311412384c237417d10ac3e2023-07-12T19:55:59ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972022-01-0166645246410.36233/0507-4088-83416Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1)A. I. Kuznetsova0https://orcid.org/0000-0001-5299-3081K. B. Gromov1https://orcid.org/0000-0002-9316-1975D. E. Kireev2https://orcid.org/0000-0002-7896-2379A. V. Shlykova3https://orcid.org/0000-0002-1390-8021A. E. Lopatukhin4https://orcid.org/0000-0002-2826-699XE. V. Kazennova5https://orcid.org/0000-0002-7912-4270A. V. Lebedev6https://orcid.org/0000-0001-6787-9345A. S. Tumanov7https://orcid.org/0000-0002-6221-5678K. V. Kim8https://orcid.org/0000-0002-4150-2280M. R. Bobkova9https://orcid.org/0000-0001-5481-8957D.I. Ivanovsky Institute of Virology FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of RussiaD.I. Ivanovsky Institute of Virology FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of RussiaFSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor)FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor)FSBI «Central Research Institute for Epidemiology» of the Federal Service for Supervision of Consumer Rights Protection and Human Welfare (Rospotrebnadzor)D.I. Ivanovsky Institute of Virology FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of RussiaD.I. Ivanovsky Institute of Virology FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of RussiaD.I. Ivanovsky Institute of Virology FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of RussiaD.I. Ivanovsky Institute of Virology FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of RussiaD.I. Ivanovsky Institute of Virology FSBI «National Research Centre for Epidemiology and Microbiology named after the honorary academician N.F. Gamaleya» of the Ministry of Health of RussiaIntroduction. Tat protein is a major factor of HIV (human immunodeficiency virus) transcription regulation and has other activities. Tat is characterized by high variability, with some amino acid substitutions, including subtypespecific ones, being able to influence on its functionality. HIV type 1 (HIV-1) sub-subtype A6 is the most widespread in Russia. Previous studies of the polymorphisms in structural regions of the A6 variant have shown numerous characteristic features; however, Tat polymorphism in A6 has not been studied. Goals and tasks. The main goal of the work was to analyze the characteristics of Tat protein in HIV-1 A6 variant, that is, to identify substitutions characteristic for A6 and A1 variants, as well as to compare the frequency of mutations in functionally significant domains in sub-subtype A6 and subtype B. Material and methods. The nucleotide sequences of HIV-1 sub-subtypes A6, A1, A2, A3, A4, subtype B and the reference nucleotide sequence were obtained from the Los Alamos international database. Results and discussion. Q54H and Q60H were identified as characteristic substitutions. Essential differences in natural polymorphisms between sub-subtypes A6 and A1 have been demonstrated. In the CPP-region, there were detected mutations (R53K, Q54H, Q54P, R57G) which were more common in sub-subtype A6 than in subtype B. Conclusion. Tat protein of sub-subtype A6 have some characteristics that make it possible to reliably distinguish it from other HIV-1 variants. Mutations identified in the CPP region could potentially alter the activity of Tat. The data obtained could form the basis for the drugs and vaccines development.https://virusjour.crie.ru/jour/article/viewFile/579/358human immunodeficiency virus type 1 (hiv-1)sub-subtype a6mutationspolymorphismtat protein |
| spellingShingle | A. I. Kuznetsova K. B. Gromov D. E. Kireev A. V. Shlykova A. E. Lopatukhin E. V. Kazennova A. V. Lebedev A. S. Tumanov K. V. Kim M. R. Bobkova Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1) Вопросы вирусологии human immunodeficiency virus type 1 (hiv-1) sub-subtype a6 mutations polymorphism tat protein |
| title | Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1) |
| title_full | Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1) |
| title_fullStr | Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1) |
| title_full_unstemmed | Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1) |
| title_short | Analysis of Tat protein characteristics in human immunodeficiency virus type 1 sub-subtype A6 (Retroviridae: Orthoretrovirinae: Lentivirus: Human immunodeficiency virus-1) |
| title_sort | analysis of tat protein characteristics in human immunodeficiency virus type 1 sub subtype a6 retroviridae orthoretrovirinae lentivirus human immunodeficiency virus 1 |
| topic | human immunodeficiency virus type 1 (hiv-1) sub-subtype a6 mutations polymorphism tat protein |
| url | https://virusjour.crie.ru/jour/article/viewFile/579/358 |
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