<b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer culture
An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) fr...
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| Format: | Article |
| Language: | English |
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Universidade Estadual de Maringá
2015-08-01
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| Series: | Acta Scientiarum: Biological Sciences |
| Subjects: | |
| Online Access: | http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/27276 |
| _version_ | 1828197892594073600 |
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| author | Lucas Roberto Pereira Gomes Cristina do Rosário Batista Franceschi Luciana Lopes Fortes Ribas |
| author_facet | Lucas Roberto Pereira Gomes Cristina do Rosário Batista Franceschi Luciana Lopes Fortes Ribas |
| author_sort | Lucas Roberto Pereira Gomes |
| collection | DOAJ |
| description | An efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival). |
| first_indexed | 2024-04-12T10:28:26Z |
| format | Article |
| id | doaj.art-877001a7b61a42e796a6706b32a81d34 |
| institution | Directory Open Access Journal |
| issn | 1679-9283 1807-863X |
| language | English |
| last_indexed | 2024-04-12T10:28:26Z |
| publishDate | 2015-08-01 |
| publisher | Universidade Estadual de Maringá |
| record_format | Article |
| series | Acta Scientiarum: Biological Sciences |
| spelling | doaj.art-877001a7b61a42e796a6706b32a81d342022-12-22T03:36:55ZengUniversidade Estadual de MaringáActa Scientiarum: Biological Sciences1679-92831807-863X2015-08-0137214314910.4025/actascibiolsci.v37i2.2727612436<b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer cultureLucas Roberto Pereira Gomes0Cristina do Rosário Batista Franceschi1Luciana Lopes Fortes Ribas2Universidade Federal do ParanáUniversidade Federal do ParanáUniversidade Federal do ParanáAn efficient in vitro propagation method was established for Brasilidium forbesii (Hook.) Campacci using transverse and longitudinal thin cell layer (tTCL and lTCL, respectively) culture systems. Six-month-old protocorms from in vitro germinated seeds were used for this study. TCLs (1.0-mm thick) from protocorms were grown on Woody Plant Medium (WPM) supplemented with benzyladenine (BA) (0.5–4.0 µM).The lTCL technique was more efficient for inducing protocorm-like bodies (PLBs) and regenerating shoots than the tTCL technique. The frequency of PLB formation was influenced by BA concentration, and the lTCL explant grown on a medium containing 2.0 µM BA produced the highest percentage of new protocorms (77%) with a total of 22.7 PLBs per explant, after the first subculture on the same medium. Plantlet development was optimal on WPM medium containing 3.0 g L-1 activated charcoal, and indole-3-butyric acid was not necessary for rooting. Regenerated plants were successfully acclimatized in a greenhouse after 16 weeks using vermiculite as the substrate (100% survival).http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/27276epiphytic orchidin vitro propagationprotocormsprotocorm-like body |
| spellingShingle | Lucas Roberto Pereira Gomes Cristina do Rosário Batista Franceschi Luciana Lopes Fortes Ribas <b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer culture Acta Scientiarum: Biological Sciences epiphytic orchid in vitro propagation protocorms protocorm-like body |
| title | <b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer culture |
| title_full | <b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer culture |
| title_fullStr | <b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer culture |
| title_full_unstemmed | <b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer culture |
| title_short | <b>Micropropagation of <i>Brasilidium forbesii</i> (Orchidaceae) through transverse and longitudinal thin cell layer culture |
| title_sort | b micropropagation of i brasilidium forbesii i orchidaceae through transverse and longitudinal thin cell layer culture |
| topic | epiphytic orchid in vitro propagation protocorms protocorm-like body |
| url | http://186.233.154.254/ojs/index.php/ActaSciBiolSci/article/view/27276 |
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