A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7
<i>E. coli</i> O157:H7, one of the major foodborne pathogens, can cause a significant threat to the safety of foods. The aim of this research is to develop an activated biochar-based immunosensor that can rapidly detect <i>E. coli</i> O157:H7 cells without incubation in pure...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/908 |
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| author | Abdus Sobhan Fei Jia Lisa Cooney Kelso Sonatan Kumar Biswas Kasiviswanathan Muthukumarappan Changyong Cao Lin Wei Yanbin Li |
| author_facet | Abdus Sobhan Fei Jia Lisa Cooney Kelso Sonatan Kumar Biswas Kasiviswanathan Muthukumarappan Changyong Cao Lin Wei Yanbin Li |
| author_sort | Abdus Sobhan |
| collection | DOAJ |
| description | <i>E. coli</i> O157:H7, one of the major foodborne pathogens, can cause a significant threat to the safety of foods. The aim of this research is to develop an activated biochar-based immunosensor that can rapidly detect <i>E. coli</i> O157:H7 cells without incubation in pure culture. Biochar was developed from corn stalks using proprietary reactors and then activated using steam-activation treatment. The developed activated biochar presented an enhanced surface area of 830.78 m<sup>2</sup>/g. To develop the biosensor, the gold electrode of the sensor was first coated with activated biochar and then functionalized with streptavidin as a linker and further immobilized with biotin-labeled anti-<i>E. coli</i> polyclonal antibodies (pAbs). The optimum concentration of activated biochar for sensor development was determined to be 20 mg/mL. Binding of anti-<i>E. coli</i> pAbs with <i>E. coli</i> O157:H7 resulted in a significant increase in impedance amplitude from 3.5 to 8.5 kΩ when compared to an only activated biochar-coated electrode. The developed immunosensor was able to detect <i>E. coli</i> O157:H7 cells with a limit of detection of 4 log CFU/mL without incubation. Successful binding of <i>E. coli</i> O157:H7 onto an activated biochar-based immunosensor was observed on the microelectrode surface in scanning electron microscopy (SEM) images. |
| first_indexed | 2024-03-09T20:35:05Z |
| format | Article |
| id | doaj.art-877143eaa7f145cca98a224c9c6bb8d8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:35:05Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-877143eaa7f145cca98a224c9c6bb8d82023-11-23T23:12:36ZengMDPI AGBiosensors2079-63742022-10-01121090810.3390/bios12100908A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7Abdus Sobhan0Fei Jia1Lisa Cooney Kelso2Sonatan Kumar Biswas3Kasiviswanathan Muthukumarappan4Changyong Cao5Lin Wei6Yanbin Li7Department of Biological and Agricultural Engineering, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USADepartment of Biological and Agricultural Engineering, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USADepartment of Biological and Agricultural Engineering, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USADepartment of Biological and Agricultural Engineering, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USADepartment of Agricultural and Biosystems Engineering, South Dakota State University, Brookings, SD 57007, USADepartment of Mechanical & Aerospace Engineering, Case Western Reserve University, Cleveland, OH 44106, USADepartment of Agricultural and Biosystems Engineering, South Dakota State University, Brookings, SD 57007, USADepartment of Biological and Agricultural Engineering, Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA<i>E. coli</i> O157:H7, one of the major foodborne pathogens, can cause a significant threat to the safety of foods. The aim of this research is to develop an activated biochar-based immunosensor that can rapidly detect <i>E. coli</i> O157:H7 cells without incubation in pure culture. Biochar was developed from corn stalks using proprietary reactors and then activated using steam-activation treatment. The developed activated biochar presented an enhanced surface area of 830.78 m<sup>2</sup>/g. To develop the biosensor, the gold electrode of the sensor was first coated with activated biochar and then functionalized with streptavidin as a linker and further immobilized with biotin-labeled anti-<i>E. coli</i> polyclonal antibodies (pAbs). The optimum concentration of activated biochar for sensor development was determined to be 20 mg/mL. Binding of anti-<i>E. coli</i> pAbs with <i>E. coli</i> O157:H7 resulted in a significant increase in impedance amplitude from 3.5 to 8.5 kΩ when compared to an only activated biochar-coated electrode. The developed immunosensor was able to detect <i>E. coli</i> O157:H7 cells with a limit of detection of 4 log CFU/mL without incubation. Successful binding of <i>E. coli</i> O157:H7 onto an activated biochar-based immunosensor was observed on the microelectrode surface in scanning electron microscopy (SEM) images.https://www.mdpi.com/2079-6374/12/10/908electrochemical immunosensorrapid detectionactivated biocharbiosensor<i>Escherichia coli</i> O157:H7 |
| spellingShingle | Abdus Sobhan Fei Jia Lisa Cooney Kelso Sonatan Kumar Biswas Kasiviswanathan Muthukumarappan Changyong Cao Lin Wei Yanbin Li A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7 Biosensors electrochemical immunosensor rapid detection activated biochar biosensor <i>Escherichia coli</i> O157:H7 |
| title | A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7 |
| title_full | A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7 |
| title_fullStr | A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7 |
| title_full_unstemmed | A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7 |
| title_short | A Novel Activated Biochar-Based Immunosensor for Rapid Detection of <i>E. coli</i> O157:H7 |
| title_sort | novel activated biochar based immunosensor for rapid detection of i e coli i o157 h7 |
| topic | electrochemical immunosensor rapid detection activated biochar biosensor <i>Escherichia coli</i> O157:H7 |
| url | https://www.mdpi.com/2079-6374/12/10/908 |
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