Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericata

Objective: Forensic entomological practices rely upon accurate larval identification and measurement of larval length, for the estimation of post-mortem intervals. The methods used for killing larvae may affect the length of larvae. In the autopsy hall, corpses which are contain entomological remain...

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Main Authors: Halide Nihal Açıkgöz, Ali Açıkgöz
Format: Article
Language:English
Published: Adli Tıp Uzmanları Derneği 2018-04-01
Series:Adli Tıp Bülteni
Subjects:
Online Access:http://www.adlitipbulteni.com/index.php/atb/article/view/1167
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author Halide Nihal Açıkgöz
Ali Açıkgöz
author_facet Halide Nihal Açıkgöz
Ali Açıkgöz
author_sort Halide Nihal Açıkgöz
collection DOAJ
description Objective: Forensic entomological practices rely upon accurate larval identification and measurement of larval length, for the estimation of post-mortem intervals. The methods used for killing larvae may affect the length of larvae. In the autopsy hall, corpses which are contain entomological remains have been washed with grape vinegar. Besides, while collecting and killing the larvae on corpses, crime scene teams use ethanol 70% because it is practical. The aim of this study was to determine which of hot water (90°C), cold vinegar and cold ethanol 96% method, preserved the best the length of larvae. Materials and Methods: To achieve this aim, third instar larvae which are reared on 200 g of veal meat were killed using hot water, cold vinegar and cold ethanol. Before killing and after killing the maggots, their length was measured. To determine the difference between the groups to be compared ANOVA test, to reliability and validity analyses Kruskal-Wallis and whether there was any difference between the groups were made with Tukey’s Honestly Significant Difference (Tukey’s HSD) Results and Conclusion: Hot water was found to preserve the length of the larvae more accurately than cold vinegar and ethanol. Forensic medicine experts and crime scene teams should kill the larvae collected from a corpse for entomological examination using hot water, dry them in paper towels and preserve them in 96% ethanol and then send them to a forensic entomologist.
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spelling doaj.art-877195971bc84f9d8f9a31d76f9599c92023-02-15T16:14:48ZengAdli Tıp Uzmanları DerneğiAdli Tıp Bülteni1300-865X2149-45332018-04-0123110.17986/blm.2017134561Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericataHalide Nihal Açıkgöz0Ali Açıkgöz1Ankara University, Institute of Forensic Sciences, AnkaraTurkish Rebuplic of Health Ministry, Havacılar Family Health CenterObjective: Forensic entomological practices rely upon accurate larval identification and measurement of larval length, for the estimation of post-mortem intervals. The methods used for killing larvae may affect the length of larvae. In the autopsy hall, corpses which are contain entomological remains have been washed with grape vinegar. Besides, while collecting and killing the larvae on corpses, crime scene teams use ethanol 70% because it is practical. The aim of this study was to determine which of hot water (90°C), cold vinegar and cold ethanol 96% method, preserved the best the length of larvae. Materials and Methods: To achieve this aim, third instar larvae which are reared on 200 g of veal meat were killed using hot water, cold vinegar and cold ethanol. Before killing and after killing the maggots, their length was measured. To determine the difference between the groups to be compared ANOVA test, to reliability and validity analyses Kruskal-Wallis and whether there was any difference between the groups were made with Tukey’s Honestly Significant Difference (Tukey’s HSD) Results and Conclusion: Hot water was found to preserve the length of the larvae more accurately than cold vinegar and ethanol. Forensic medicine experts and crime scene teams should kill the larvae collected from a corpse for entomological examination using hot water, dry them in paper towels and preserve them in 96% ethanol and then send them to a forensic entomologist.http://www.adlitipbulteni.com/index.php/atb/article/view/1167Forensic entomologyLucilia sericataPostmortem intervalLarval killing methods
spellingShingle Halide Nihal Açıkgöz
Ali Açıkgöz
Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericata
Adli Tıp Bülteni
Forensic entomology
Lucilia sericata
Postmortem interval
Larval killing methods
title Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericata
title_full Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericata
title_fullStr Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericata
title_full_unstemmed Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericata
title_short Effects of Killing Liquids on the Larval Length of Forensically Important Lucilia sericata
title_sort effects of killing liquids on the larval length of forensically important lucilia sericata
topic Forensic entomology
Lucilia sericata
Postmortem interval
Larval killing methods
url http://www.adlitipbulteni.com/index.php/atb/article/view/1167
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