Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis
Objectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Materials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy contr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Spanish |
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Instituto Nacional de Salud
2020-03-01
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| Series: | Revista Peruana de Medicina Experimental y Salud Pública |
| Subjects: | |
| Online Access: | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4468 |
| _version_ | 1828777996933136384 |
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| author | Jose Yareta Marco Galarza Silvia Capristano Oscar Pellón César Sánchez Jorge Ballon Heinner Guio |
| author_facet | Jose Yareta Marco Galarza Silvia Capristano Oscar Pellón César Sánchez Jorge Ballon Heinner Guio |
| author_sort | Jose Yareta |
| collection | DOAJ |
| description | Objectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Materials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy controls) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Results: Overexpression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). Conclusion: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings. |
| first_indexed | 2024-12-11T16:33:17Z |
| format | Article |
| id | doaj.art-8772922fc6dd476a83d74438e1ae05ab |
| institution | Directory Open Access Journal |
| issn | 1726-4634 1726-4642 |
| language | Spanish |
| last_indexed | 2024-12-11T16:33:17Z |
| publishDate | 2020-03-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Revista Peruana de Medicina Experimental y Salud Pública |
| spelling | doaj.art-8772922fc6dd476a83d74438e1ae05ab2022-12-22T00:58:33ZspaInstituto Nacional de SaludRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422020-03-0137151610.17843/rpmesp.2020.371.44682306Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosisJose Yareta0Marco Galarza1Silvia Capristano2Oscar Pellón3César Sánchez4Jorge Ballon5Heinner Guio6Laboratorio de Referencia Nacional de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú. Departamento Académico de Biología, Universidad Nacional de San Agustín de Arequipa, Arequipa, Perú. BiólogoLaboratorio de Referencia Nacional de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú. BiólogoLaboratorio de Referencia Nacional de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú. tecnólogo médico con especialidad en Laboratorio ClínicoLaboratorio de Referencia Nacional de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú. biólogo molecular, PhD en Biología Celular y Molecular del Cáncer.Laboratorio de Referencia Nacional de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú. médico cirujano; Maestría en CienciasDepartamento Académico de Biología, Universidad Nacional de San Agustín de Arequipa, Arequipa, Perú. médico cirujano; Maestría en CienciasLaboratorio de Referencia Nacional de Biotecnología y Biología Molecular, Instituto Nacional de Salud, Lima, Perú. médico cirujano PhD en Ciencias MédicasObjectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Materials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy controls) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Results: Overexpression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). Conclusion: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4468tuberculosis latentetuberculosismicroarnsperú |
| spellingShingle | Jose Yareta Marco Galarza Silvia Capristano Oscar Pellón César Sánchez Jorge Ballon Heinner Guio Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis Revista Peruana de Medicina Experimental y Salud Pública tuberculosis latente tuberculosis microarns perú |
| title | Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis |
| title_full | Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis |
| title_fullStr | Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis |
| title_full_unstemmed | Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis |
| title_short | Differential expression of circulating micro-RNAs in patients with active tuberculosis and infected with latent tuberculosis |
| title_sort | differential expression of circulating micro rnas in patients with active tuberculosis and infected with latent tuberculosis |
| topic | tuberculosis latente tuberculosis microarns perú |
| url | https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4468 |
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