Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple Leaf
Glomerella leaf spot of apple, caused by <i>Colletotrichum</i><i>gloeosporioides</i>, is a devastating disease that leads to severe defoliation and fruit spots. The <i>Colletotrichum</i> species secretes a series of effectors to manipulate the host’s immune respon...
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/12/6379 |
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| author | Meiyu Wang Zhirui Ji Haifeng Yan Jie Xu Xuanzhu Zhao Zongshan Zhou |
| author_facet | Meiyu Wang Zhirui Ji Haifeng Yan Jie Xu Xuanzhu Zhao Zongshan Zhou |
| author_sort | Meiyu Wang |
| collection | DOAJ |
| description | Glomerella leaf spot of apple, caused by <i>Colletotrichum</i><i>gloeosporioides</i>, is a devastating disease that leads to severe defoliation and fruit spots. The <i>Colletotrichum</i> species secretes a series of effectors to manipulate the host’s immune response, facilitating its colonization in plants. However, the mechanism by which the effector of <i>C. gloeosporioides</i> inhibits the defenses of the host remains unclear. In this study, we reported a novel effector Sntf2 of <i>C. gloeosporioides</i>. The transient expression of <i>S</i><i>NTF2</i> inhibits BAX-induced cell death in tobacco plants. Sntf2 suppresses plant defense responses by reducing callose deposition and H<sub>2</sub>O<sub>2</sub> accumulation. <i>SNTF2</i> is upregulated during infection, and its deletion reduces virulence to the plant. Sntf2 is localized to the chloroplasts and interacts with Mdycf39 (a chloroplast PSII assembly factor) in apple leaves. The <i>Mdycf39</i> overexpression line increases susceptibility to <i>C. gloeosporioides</i>, whereas the <i>Mdycf39</i> transgenic silent line does not grow normally with pale white leaves, indicating that Sntf2 disturbs plant defense responses and growth by targeting Mdycf39. |
| first_indexed | 2024-03-09T23:37:06Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:37:06Z |
| publishDate | 2022-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8779e8875e1442cc8576a70c2e8dad502023-11-23T16:59:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312637910.3390/ijms23126379Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple LeafMeiyu Wang0Zhirui Ji1Haifeng Yan2Jie Xu3Xuanzhu Zhao4Zongshan Zhou5Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaResearch Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, ChinaGlomerella leaf spot of apple, caused by <i>Colletotrichum</i><i>gloeosporioides</i>, is a devastating disease that leads to severe defoliation and fruit spots. The <i>Colletotrichum</i> species secretes a series of effectors to manipulate the host’s immune response, facilitating its colonization in plants. However, the mechanism by which the effector of <i>C. gloeosporioides</i> inhibits the defenses of the host remains unclear. In this study, we reported a novel effector Sntf2 of <i>C. gloeosporioides</i>. The transient expression of <i>S</i><i>NTF2</i> inhibits BAX-induced cell death in tobacco plants. Sntf2 suppresses plant defense responses by reducing callose deposition and H<sub>2</sub>O<sub>2</sub> accumulation. <i>SNTF2</i> is upregulated during infection, and its deletion reduces virulence to the plant. Sntf2 is localized to the chloroplasts and interacts with Mdycf39 (a chloroplast PSII assembly factor) in apple leaves. The <i>Mdycf39</i> overexpression line increases susceptibility to <i>C. gloeosporioides</i>, whereas the <i>Mdycf39</i> transgenic silent line does not grow normally with pale white leaves, indicating that Sntf2 disturbs plant defense responses and growth by targeting Mdycf39.https://www.mdpi.com/1422-0067/23/12/6379<i>Colletotrichum</i>effectorpathogenicityimmune responseapple |
| spellingShingle | Meiyu Wang Zhirui Ji Haifeng Yan Jie Xu Xuanzhu Zhao Zongshan Zhou Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple Leaf International Journal of Molecular Sciences <i>Colletotrichum</i> effector pathogenicity immune response apple |
| title | Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple Leaf |
| title_full | Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple Leaf |
| title_fullStr | Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple Leaf |
| title_full_unstemmed | Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple Leaf |
| title_short | Effector Sntf2 Interacted with Chloroplast-Related Protein Mdycf39 Promoting the Colonization of <i>Colletotrichum gloeosporioides</i> in Apple Leaf |
| title_sort | effector sntf2 interacted with chloroplast related protein mdycf39 promoting the colonization of i colletotrichum gloeosporioides i in apple leaf |
| topic | <i>Colletotrichum</i> effector pathogenicity immune response apple |
| url | https://www.mdpi.com/1422-0067/23/12/6379 |
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