Detection of Minority Variants and Mixed Infections in Mycobacterium tuberculosis by Direct Whole-Genome Sequencing on Noncultured Specimens Using a Specific-DNA Capture Strategy

Detection of Minority Variants and Mixed Infections in Mycobacterium tuberculosis by Direct Whole-Genome Sequencing on Noncultured Specimens Using a Specific-DNA Capture Strategy

ABSTRACT Detection of mixed Mycobacterium tuberculosis (MTB) infections is essential, particularly when resistance mutations are present in minority bacterial populations that may affect patients’ disease evolution and treatment. Whole-genome sequencing (WGS) has extended the amount of key informati...

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Main Authors: Nuria Lozano, Val F. Lanza, Julia Suárez-González, Marta Herranz, Pedro J. Sola-Campoy, Cristina Rodríguez-Grande, Sergio Buenestado-Serrano, María Jesús Ruiz-Serrano, Griselda Tudó, Fernando Alcaide, Patricia Muñoz, Darío García de Viedma, Laura Pérez-Lago
Format: Article
Language:English
Published: American Society for Microbiology 2021-12-01
Series:mSphere
Subjects:
Mycobacterium tuberculosis
whole-genome sequencing
heteroresistance
mixed infections
specific-DNA capture
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00744-21

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