Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice
The objective of this study was to evaluate the effect of organic and conventional grape juices consumption on the behavior of rats and their neuroprotective effect on the activity of brain energy metabolism enzymes in different brain areas of adult rats on the experimental model of hepatic encephal...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Toxicology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750015000438 |
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| author | Tatiane Gabardo Clarice M. Peripolli Rodrigo Binkowski de Andrade Tanise Gemelli Juliana D.O. Lima Alice S. Oliveira Niara da Silva Medeiros Clovis Wannmacher Caroline Dani Cláudia Funchal |
| author_facet | Tatiane Gabardo Clarice M. Peripolli Rodrigo Binkowski de Andrade Tanise Gemelli Juliana D.O. Lima Alice S. Oliveira Niara da Silva Medeiros Clovis Wannmacher Caroline Dani Cláudia Funchal |
| author_sort | Tatiane Gabardo |
| collection | DOAJ |
| description | The objective of this study was to evaluate the effect of organic and conventional grape juices consumption on the behavior of rats and their neuroprotective effect on the activity of brain energy metabolism enzymes in different brain areas of adult rats on the experimental model of hepatic encephalopathy. Male Wistar rats (90-days-old) were treated once a day with conventional or organic white grape juice by gavage for 14 days (7 μL/g). On the 15th day the rats received carbon tetrachloride (CCl4) in a single dose of 3.0 mL/kg. Cerebral cortex, hippocampus and cerebellum were dissected to measure the activity of creatine kinase (CK) and pyruvate kinase (PK). No changes in feeding behavior were observed after the treatment with the grapes juices. However, there was an increase in grooming behavior in the open field test provoked by both juices. CCl4 inhibited CK activity in cerebral cortex and hippocampus of the rats and CCl4 also reduced PK activity in all brain structures studied. Furthermore, both white grape juices prevented the decrease in the activity of CK and PK. Therefore, we can suggest that organic and conventional white grape juices could restore the activity of enzymes with a central role in brain energy metabolism. |
| first_indexed | 2024-12-11T02:47:30Z |
| format | Article |
| id | doaj.art-877e6eca3a39404d84def6baf0779cac |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-12-11T02:47:30Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-877e6eca3a39404d84def6baf0779cac2022-12-22T01:23:24ZengElsevierToxicology Reports2214-75002015-01-012C64565310.1016/j.toxrep.2015.03.011Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juiceTatiane Gabardo0Clarice M. Peripolli1Rodrigo Binkowski de Andrade2Tanise Gemelli3Juliana D.O. Lima4Alice S. Oliveira5Niara da Silva Medeiros6Clovis Wannmacher7Caroline Dani8Cláudia Funchal9Centro Universitário Metodista – IPA, Porto Alegre, RS, BrazilCentro Universitário Metodista – IPA, Porto Alegre, RS, BrazilDepartamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilDepartamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro Universitário Metodista – IPA, Porto Alegre, RS, BrazilCentro Universitário Metodista – IPA, Porto Alegre, RS, BrazilCentro Universitário Metodista – IPA, Porto Alegre, RS, BrazilDepartamento de Bioquímica, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, BrazilCentro Universitário Metodista – IPA, Porto Alegre, RS, BrazilCentro Universitário Metodista – IPA, Porto Alegre, RS, BrazilThe objective of this study was to evaluate the effect of organic and conventional grape juices consumption on the behavior of rats and their neuroprotective effect on the activity of brain energy metabolism enzymes in different brain areas of adult rats on the experimental model of hepatic encephalopathy. Male Wistar rats (90-days-old) were treated once a day with conventional or organic white grape juice by gavage for 14 days (7 μL/g). On the 15th day the rats received carbon tetrachloride (CCl4) in a single dose of 3.0 mL/kg. Cerebral cortex, hippocampus and cerebellum were dissected to measure the activity of creatine kinase (CK) and pyruvate kinase (PK). No changes in feeding behavior were observed after the treatment with the grapes juices. However, there was an increase in grooming behavior in the open field test provoked by both juices. CCl4 inhibited CK activity in cerebral cortex and hippocampus of the rats and CCl4 also reduced PK activity in all brain structures studied. Furthermore, both white grape juices prevented the decrease in the activity of CK and PK. Therefore, we can suggest that organic and conventional white grape juices could restore the activity of enzymes with a central role in brain energy metabolism.http://www.sciencedirect.com/science/article/pii/S2214750015000438AntioxidantsCreatine kinasePyruvate kinaseFeeding behaviorOpen field test |
| spellingShingle | Tatiane Gabardo Clarice M. Peripolli Rodrigo Binkowski de Andrade Tanise Gemelli Juliana D.O. Lima Alice S. Oliveira Niara da Silva Medeiros Clovis Wannmacher Caroline Dani Cláudia Funchal Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice Toxicology Reports Antioxidants Creatine kinase Pyruvate kinase Feeding behavior Open field test |
| title | Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice |
| title_full | Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice |
| title_fullStr | Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice |
| title_full_unstemmed | Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice |
| title_short | Assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in Wistar rats and the protective effect of white grape juice |
| title_sort | assessment of changes in energy metabolism parameters provoked by carbon tetrachloride in wistar rats and the protective effect of white grape juice |
| topic | Antioxidants Creatine kinase Pyruvate kinase Feeding behavior Open field test |
| url | http://www.sciencedirect.com/science/article/pii/S2214750015000438 |
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