In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics
Rhizosphere arguably embodies the most diverse microbial ecosystem on the planet, yet it is largely a functional ‘black box’ of belowground plant-microbiome interactions. The rhizosphere is the primary site of entry for subsurface injection of fixed carbon (C) into soil with impacts on local to glob...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/11/11/2248 |
| _version_ | 1797511574439591936 |
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| author | Richard Allen White Joshua Rosnow Paul D. Piehowski Colin J. Brislawn James J. Moran |
| author_facet | Richard Allen White Joshua Rosnow Paul D. Piehowski Colin J. Brislawn James J. Moran |
| author_sort | Richard Allen White |
| collection | DOAJ |
| description | Rhizosphere arguably embodies the most diverse microbial ecosystem on the planet, yet it is largely a functional ‘black box’ of belowground plant-microbiome interactions. The rhizosphere is the primary site of entry for subsurface injection of fixed carbon (C) into soil with impacts on local to global scale C biogeochemistry and ultimately Earth’s climate. While spatial organization of rhizosphere is central to its function, small scale and steep microbial and geochemical gradients within this dynamic region make it easily disrupted by sampling. The significant challenge presented by sampling blocks elucidation of discreet functions, drivers, and interactions within rhizosphere ecosystems. Here, we describe a non-destructive sampling method linked to metaproteomic analysis in order to measure temporal shifts in the microbial composition and function of rhizosphere. A robust, non-destructive method of sampling microbial hotspots within rhizosphere provides an unperturbed window into the elusive functional interactome of this system over time and space. |
| first_indexed | 2024-03-10T05:47:12Z |
| format | Article |
| id | doaj.art-877e88450c9144808dffcb34ea5c4791 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T05:47:12Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-877e88450c9144808dffcb34ea5c47912023-11-22T22:02:44ZengMDPI AGAgronomy2073-43952021-11-011111224810.3390/agronomy11112248In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using MetaproteomicsRichard Allen White0Joshua Rosnow1Paul D. Piehowski2Colin J. Brislawn3James J. Moran4Department of Bioinformatics and Genomics, The University of North Carolina at Charlotte, 9201 University City Boulevard, Charlotte, NC 28223, USAPacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99354, USAPacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99354, USAPacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99354, USAPacific Northwest National Laboratory, 902 Battelle Blvd, Richland, WA 99354, USARhizosphere arguably embodies the most diverse microbial ecosystem on the planet, yet it is largely a functional ‘black box’ of belowground plant-microbiome interactions. The rhizosphere is the primary site of entry for subsurface injection of fixed carbon (C) into soil with impacts on local to global scale C biogeochemistry and ultimately Earth’s climate. While spatial organization of rhizosphere is central to its function, small scale and steep microbial and geochemical gradients within this dynamic region make it easily disrupted by sampling. The significant challenge presented by sampling blocks elucidation of discreet functions, drivers, and interactions within rhizosphere ecosystems. Here, we describe a non-destructive sampling method linked to metaproteomic analysis in order to measure temporal shifts in the microbial composition and function of rhizosphere. A robust, non-destructive method of sampling microbial hotspots within rhizosphere provides an unperturbed window into the elusive functional interactome of this system over time and space.https://www.mdpi.com/2073-4395/11/11/2248rhizospheremetaproteomicshot-spotsmetagenomicsin situnon-destructive |
| spellingShingle | Richard Allen White Joshua Rosnow Paul D. Piehowski Colin J. Brislawn James J. Moran In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics Agronomy rhizosphere metaproteomics hot-spots metagenomics in situ non-destructive |
| title | In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics |
| title_full | In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics |
| title_fullStr | In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics |
| title_full_unstemmed | In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics |
| title_short | In Situ Non-Destructive Temporal Measurements of the Rhizosphere Microbiome ‘Hot-Spots’ Using Metaproteomics |
| title_sort | in situ non destructive temporal measurements of the rhizosphere microbiome hot spots using metaproteomics |
| topic | rhizosphere metaproteomics hot-spots metagenomics in situ non-destructive |
| url | https://www.mdpi.com/2073-4395/11/11/2248 |
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