Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17
The ribosome is a large ribonucleoprotein complex that synthesizes protein in all living organisms. Ribosome biogenesis is a complex process that requires synchronization of various cellular events, including ribosomal RNA (rRNA) transcription, ribosome assembly, and processing and post-transcriptio...
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MDPI AG
2020-05-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/6/841 |
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| author | Kumudie Jayalath Sean Frisbie Minhchau To Sanjaya Abeysirigunawardena |
| author_facet | Kumudie Jayalath Sean Frisbie Minhchau To Sanjaya Abeysirigunawardena |
| author_sort | Kumudie Jayalath |
| collection | DOAJ |
| description | The ribosome is a large ribonucleoprotein complex that synthesizes protein in all living organisms. Ribosome biogenesis is a complex process that requires synchronization of various cellular events, including ribosomal RNA (rRNA) transcription, ribosome assembly, and processing and post-transcriptional modification of rRNA. Ribosome biogenesis is fine-tuned with various assembly factors, possibly including nucleotide modification enzymes. Ribosomal small subunit pseudouridine synthase A (RsuA) pseudouridylates U516 of 16S helix 18. Protein RsuA is a multi-domain protein that contains the N-terminal peripheral domain, which is structurally similar to the ribosomal protein S4. Our study shows RsuA preferably binds and pseudouridylates an assembly intermediate that is stabilized by ribosomal protein S17 over the native-like complex. In addition, the N-terminal domain truncated RsuA showed that the presence of the S4-like domain is important for RsuA substrate recognition. |
| first_indexed | 2024-03-10T19:28:48Z |
| format | Article |
| id | doaj.art-877fd54ebc0d438aa283cfa917a85495 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:28:48Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-877fd54ebc0d438aa283cfa917a854952023-11-20T02:18:38ZengMDPI AGBiomolecules2218-273X2020-05-0110684110.3390/biom10060841Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17Kumudie Jayalath0Sean Frisbie1Minhchau To2Sanjaya Abeysirigunawardena3Department of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USADepartment of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USADepartment of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USADepartment of Chemistry and Biochemistry, Kent State University, Kent, OH 44242, USAThe ribosome is a large ribonucleoprotein complex that synthesizes protein in all living organisms. Ribosome biogenesis is a complex process that requires synchronization of various cellular events, including ribosomal RNA (rRNA) transcription, ribosome assembly, and processing and post-transcriptional modification of rRNA. Ribosome biogenesis is fine-tuned with various assembly factors, possibly including nucleotide modification enzymes. Ribosomal small subunit pseudouridine synthase A (RsuA) pseudouridylates U516 of 16S helix 18. Protein RsuA is a multi-domain protein that contains the N-terminal peripheral domain, which is structurally similar to the ribosomal protein S4. Our study shows RsuA preferably binds and pseudouridylates an assembly intermediate that is stabilized by ribosomal protein S17 over the native-like complex. In addition, the N-terminal domain truncated RsuA showed that the presence of the S4-like domain is important for RsuA substrate recognition.https://www.mdpi.com/2218-273X/10/6/841bacterial ribosome assemblynucleotide modification enzymespseudouridine synthase |
| spellingShingle | Kumudie Jayalath Sean Frisbie Minhchau To Sanjaya Abeysirigunawardena Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17 Biomolecules bacterial ribosome assembly nucleotide modification enzymes pseudouridine synthase |
| title | Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17 |
| title_full | Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17 |
| title_fullStr | Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17 |
| title_full_unstemmed | Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17 |
| title_short | Pseudouridine Synthase RsuA Captures an Assembly Intermediate That Is Stabilized by Ribosomal Protein S17 |
| title_sort | pseudouridine synthase rsua captures an assembly intermediate that is stabilized by ribosomal protein s17 |
| topic | bacterial ribosome assembly nucleotide modification enzymes pseudouridine synthase |
| url | https://www.mdpi.com/2218-273X/10/6/841 |
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