Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency
This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/10/11/1105 |
| _version_ | 1797511225774440448 |
|---|---|
| author | Bruna Resende Chaves Ana Paula Pinoti Pavaneli Olga Blanco-Prieto Elisabeth Pinart Sergi Bonet Márcio Gilberto Zangeronimo Joan E. Rodríguez-Gil Marc Yeste |
| author_facet | Bruna Resende Chaves Ana Paula Pinoti Pavaneli Olga Blanco-Prieto Elisabeth Pinart Sergi Bonet Márcio Gilberto Zangeronimo Joan E. Rodríguez-Gil Marc Yeste |
| author_sort | Bruna Resende Chaves |
| collection | DOAJ |
| description | This work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with different concentrations of bicarbonate (either 0 mM, 5 mM, 15 mM or 38 mM) and BSA (either 0 mg/mL or 5 mg/mL). The achievement of in vitro capacitation and progesterone-induced acrosomal exocytosis was tested through the analysis of sperm motility, plasma membrane integrity and lipid disorder, acrosome exocytosis, intracellular calcium levels, mitochondria membrane potential, O<sub>2</sub> consumption rate and the activities of both glycogen synthase kinase 3 alpha (GSK3α) and protein kinase A (PKA). While sperm incubated in media without BSA or BSA/bicarbonate, they did not achieve in vitro capacitation; those incubated in media with BSA achieved the capacitated status under any bicarbonate concentration, even when bicarbonate was absent. Moreover, there were differences related to the concentration of bicarbonate, since sperm incubated in media with BSA and with no bicarbonate or 5 mM bicarbonate showed lower overall efficiency in achieving in vitro capacitation than those incubated in the presence of BSA and 15 mM or 38 mM bicarbonate. Additionally, at the end of the experiment, sperm incubated in the presence of BSA and 38 mM bicarbonate showed significantly (<i>p</i> < 0.05) lower values of motility and plasma membrane integrity than those incubated in media with BSA and lower concentrations of bicarbonate. In conclusion, BSA is instrumental for pig sperm to elicit in vitro capacitation and trigger the subsequent progesterone-induced acrosome exocytosis. Furthermore, while exogenous bicarbonate does not seem to be essential to launch sperm capacitation, it does modulate its efficiency. |
| first_indexed | 2024-03-10T05:42:21Z |
| format | Article |
| id | doaj.art-87814e21f40f42fa98b6f656a772243a |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T05:42:21Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-87814e21f40f42fa98b6f656a772243a2023-11-22T22:27:25ZengMDPI AGBiology2079-77372021-10-011011110510.3390/biology10111105Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its EfficiencyBruna Resende Chaves0Ana Paula Pinoti Pavaneli1Olga Blanco-Prieto2Elisabeth Pinart3Sergi Bonet4Márcio Gilberto Zangeronimo5Joan E. Rodríguez-Gil6Marc Yeste7Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, SpainBiotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, SpainUnit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, ES-08193 Bellaterra (Cerdanyola del Vallès), SpainBiotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, SpainBiotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, SpainDepartment of Veterinary Medicine, Federal University of Lavras, BR-37200-000 Lavras, MG, BrazilUnit of Animal Reproduction, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, ES-08193 Bellaterra (Cerdanyola del Vallès), SpainBiotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, ES-17003 Girona, SpainThis work sought to address whether the presence of exogenous bicarbonate is required for pig sperm to elicit in vitro capacitation and further progesterone-induced acrosome exocytosis. For this purpose, sperm were either incubated in a standard in vitro capacitation medium or a similar medium with different concentrations of bicarbonate (either 0 mM, 5 mM, 15 mM or 38 mM) and BSA (either 0 mg/mL or 5 mg/mL). The achievement of in vitro capacitation and progesterone-induced acrosomal exocytosis was tested through the analysis of sperm motility, plasma membrane integrity and lipid disorder, acrosome exocytosis, intracellular calcium levels, mitochondria membrane potential, O<sub>2</sub> consumption rate and the activities of both glycogen synthase kinase 3 alpha (GSK3α) and protein kinase A (PKA). While sperm incubated in media without BSA or BSA/bicarbonate, they did not achieve in vitro capacitation; those incubated in media with BSA achieved the capacitated status under any bicarbonate concentration, even when bicarbonate was absent. Moreover, there were differences related to the concentration of bicarbonate, since sperm incubated in media with BSA and with no bicarbonate or 5 mM bicarbonate showed lower overall efficiency in achieving in vitro capacitation than those incubated in the presence of BSA and 15 mM or 38 mM bicarbonate. Additionally, at the end of the experiment, sperm incubated in the presence of BSA and 38 mM bicarbonate showed significantly (<i>p</i> < 0.05) lower values of motility and plasma membrane integrity than those incubated in media with BSA and lower concentrations of bicarbonate. In conclusion, BSA is instrumental for pig sperm to elicit in vitro capacitation and trigger the subsequent progesterone-induced acrosome exocytosis. Furthermore, while exogenous bicarbonate does not seem to be essential to launch sperm capacitation, it does modulate its efficiency.https://www.mdpi.com/2079-7737/10/11/1105sperm capacitationpigbicarbonatebovine serum albumin (BSA)glycogen synthase kinase (GSK3)protein kinase A (PKA) |
| spellingShingle | Bruna Resende Chaves Ana Paula Pinoti Pavaneli Olga Blanco-Prieto Elisabeth Pinart Sergi Bonet Márcio Gilberto Zangeronimo Joan E. Rodríguez-Gil Marc Yeste Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency Biology sperm capacitation pig bicarbonate bovine serum albumin (BSA) glycogen synthase kinase (GSK3) protein kinase A (PKA) |
| title | Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency |
| title_full | Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency |
| title_fullStr | Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency |
| title_full_unstemmed | Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency |
| title_short | Exogenous Albumin Is Crucial for Pig Sperm to Elicit In Vitro Capacitation Whereas Bicarbonate Only Modulates Its Efficiency |
| title_sort | exogenous albumin is crucial for pig sperm to elicit in vitro capacitation whereas bicarbonate only modulates its efficiency |
| topic | sperm capacitation pig bicarbonate bovine serum albumin (BSA) glycogen synthase kinase (GSK3) protein kinase A (PKA) |
| url | https://www.mdpi.com/2079-7737/10/11/1105 |
| work_keys_str_mv | AT brunaresendechaves exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency AT anapaulapinotipavaneli exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency AT olgablancoprieto exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency AT elisabethpinart exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency AT sergibonet exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency AT marciogilbertozangeronimo exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency AT joanerodriguezgil exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency AT marcyeste exogenousalbuminiscrucialforpigspermtoelicitinvitrocapacitationwhereasbicarbonateonlymodulatesitsefficiency |