A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins
The level of C-reactive protein (CRP) in the human body is closely associated with cardiovascular diseases and inflammation. In this study, a label-free functionalized aptamer sensor was attached to an electrode trimmed with in-gold nanoparticles and carboxylated graphene oxide (AuNPs/GO-COOH) to ac...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/12/1180 |
| _version_ | 1797461153181335552 |
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| author | Huilin Gao Yongchang Bai Baixun He Cherie S. Tan |
| author_facet | Huilin Gao Yongchang Bai Baixun He Cherie S. Tan |
| author_sort | Huilin Gao |
| collection | DOAJ |
| description | The level of C-reactive protein (CRP) in the human body is closely associated with cardiovascular diseases and inflammation. In this study, a label-free functionalized aptamer sensor was attached to an electrode trimmed with in-gold nanoparticles and carboxylated graphene oxide (AuNPs/GO-COOH) to achieve sensitive measurements relative to CRP. Gold nanoparticles were selected for this study due to super stability, remarkably high electrical conductivity, and biocompatibility. In addition, carboxylated graphene oxide was utilized to promote the anchorage of inducer molecules and to increase detection accuracies. The sensing signal was recorded using differential pulse voltammetry (DPV), and it produced a conspicuous peak current obtained at approximately −0.4 V. Furthermore, the adapted sensor manifested a broad linear span from 0.001 ng/mL to 100 ng/mL. The results also demonstrated that this aptamer sensor had superior stability, specificity, and reproducibility. This aptamer-based electrochemical sensor has enormous potential in complex application situations with interfering substances. |
| first_indexed | 2024-03-09T17:16:19Z |
| format | Article |
| id | doaj.art-8782c79adf2b4b2c9739ab490ed7ac5b |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T17:16:19Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-8782c79adf2b4b2c9739ab490ed7ac5b2023-11-24T13:37:58ZengMDPI AGBiosensors2079-63742022-12-011212118010.3390/bios12121180A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive ProteinsHuilin Gao0Yongchang Bai1Baixun He2Cherie S. Tan3Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, ChinaAcademy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, ChinaAcademy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, ChinaAcademy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, ChinaThe level of C-reactive protein (CRP) in the human body is closely associated with cardiovascular diseases and inflammation. In this study, a label-free functionalized aptamer sensor was attached to an electrode trimmed with in-gold nanoparticles and carboxylated graphene oxide (AuNPs/GO-COOH) to achieve sensitive measurements relative to CRP. Gold nanoparticles were selected for this study due to super stability, remarkably high electrical conductivity, and biocompatibility. In addition, carboxylated graphene oxide was utilized to promote the anchorage of inducer molecules and to increase detection accuracies. The sensing signal was recorded using differential pulse voltammetry (DPV), and it produced a conspicuous peak current obtained at approximately −0.4 V. Furthermore, the adapted sensor manifested a broad linear span from 0.001 ng/mL to 100 ng/mL. The results also demonstrated that this aptamer sensor had superior stability, specificity, and reproducibility. This aptamer-based electrochemical sensor has enormous potential in complex application situations with interfering substances.https://www.mdpi.com/2079-6374/12/12/1180C-reactive proteinDNA aptamergold nanoparticlescarboxylated graphene oxide |
| spellingShingle | Huilin Gao Yongchang Bai Baixun He Cherie S. Tan A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins Biosensors C-reactive protein DNA aptamer gold nanoparticles carboxylated graphene oxide |
| title | A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins |
| title_full | A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins |
| title_fullStr | A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins |
| title_full_unstemmed | A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins |
| title_short | A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins |
| title_sort | simple label free aptamer based electrochemical biosensor for the sensitive detection of c reactive proteins |
| topic | C-reactive protein DNA aptamer gold nanoparticles carboxylated graphene oxide |
| url | https://www.mdpi.com/2079-6374/12/12/1180 |
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