Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regeneration
Background: Tissue engineering is a developing multidisciplinary and interdisciplinary field involving the use of bioartificial implants for tissue remodeling with the target for repair and enhancing tissue or organ function. Acellular nerve has been used in experimental models as a peripheral nerve...
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| Format: | Article |
| Language: | fas |
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Tehran University of Medical Sciences
2019-05-01
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| Series: | Tehran University Medical Journal |
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| Online Access: | http://tumj.tums.ac.ir/article-1-9638-en.html |
| _version_ | 1818954225912119296 |
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| author | Arash Abdolmaleki Mohammad-Bagher Ghayour Saber Zahri Asadollah Asadi Morteza Behnam-Rassouli |
| author_facet | Arash Abdolmaleki Mohammad-Bagher Ghayour Saber Zahri Asadollah Asadi Morteza Behnam-Rassouli |
| author_sort | Arash Abdolmaleki |
| collection | DOAJ |
| description | Background: Tissue engineering is a developing multidisciplinary and interdisciplinary field involving the use of bioartificial implants for tissue remodeling with the target for repair and enhancing tissue or organ function. Acellular nerve has been used in experimental models as a peripheral nerve substitute. The purpose of the present study was to evaluate the mechanical and histological characteristics of acellular nerve scaffolds compared to the fresh nerve for application in environmental nerve repair.
Methods: This experimental study was conducted in Ferdowsi University of Mashhad Regeneration Research Laboratory, Mashhad, Iran, from May 2017 to October 2018. In this study for preparing the scaffold. The rats were sacrificed by intraperitoneal anesthesia with 10 % Chloral Hydrate solution. Then sciatic nerve fragments of the rats were removed above the nerve branching site and after cleansing of the tissues were decellularized by Sondell method, briefly nerves were treated with a series of detergent baths consisting of distilled water for 8 h, Triton X-100 for 12 h, and sodium deoxycholate for 24 hours according to the Sondell protocol. All acellularization steps were performed at room temperature. Then decellularized scaffolds were evaluated histologically and mechanically.
Results: The results of tissue evaluations showed that decellularization of scaffolds were done completely, this was demonstrated by hematoxylin and eosin staining and DAPI staining. Also the specialized tissue evaluations by picro-fuchsin staining and evaluation the scaffolds by scanning electron microscopy (SEM) micrographs showed that the collagen and elastin strands are relatively preserved in the extracellular matrix in comparison with control groups. As well as mechanical examination of scaffolds in tensile test showed that extracellular matrix of scaffolds was relatively preserved the main components of tissue compared to control group and scaffolds have good mechanical resistance quality for use in tissue engineering.
Conclusion: The results of the present study showed that decellularized scaffolds that prepared with Sondell decellularization method by preserving the main components of the tissue can be a good platform for investigating cellular behaviors. |
| first_indexed | 2024-12-20T10:18:47Z |
| format | Article |
| id | doaj.art-8785a26cc3d9444fba97fe955e9d55c3 |
| institution | Directory Open Access Journal |
| issn | 1683-1764 1735-7322 |
| language | fas |
| last_indexed | 2024-12-20T10:18:47Z |
| publishDate | 2019-05-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Tehran University Medical Journal |
| spelling | doaj.art-8785a26cc3d9444fba97fe955e9d55c32022-12-21T19:43:59ZfasTehran University of Medical SciencesTehran University Medical Journal1683-17641735-73222019-05-01772115122Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regenerationArash Abdolmaleki0Mohammad-Bagher Ghayour1Saber Zahri2Asadollah Asadi3Morteza Behnam-Rassouli4 Department of Engineering Sciences, Faculty of Advanced Technologies, University of Mohaghegh Ardabili, Namin, Ardabil, Iran. Bio Science and Biotechnology Researchcenter (BBRC), Sabalan University of Advanced Technologies (SUAT), Namin, Ardabil, Iran. Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. Mashhad, Mashhad, Iran. 4- Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran. Mashhad, Mashhad, Iran. 4- Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran. Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. Background: Tissue engineering is a developing multidisciplinary and interdisciplinary field involving the use of bioartificial implants for tissue remodeling with the target for repair and enhancing tissue or organ function. Acellular nerve has been used in experimental models as a peripheral nerve substitute. The purpose of the present study was to evaluate the mechanical and histological characteristics of acellular nerve scaffolds compared to the fresh nerve for application in environmental nerve repair. Methods: This experimental study was conducted in Ferdowsi University of Mashhad Regeneration Research Laboratory, Mashhad, Iran, from May 2017 to October 2018. In this study for preparing the scaffold. The rats were sacrificed by intraperitoneal anesthesia with 10 % Chloral Hydrate solution. Then sciatic nerve fragments of the rats were removed above the nerve branching site and after cleansing of the tissues were decellularized by Sondell method, briefly nerves were treated with a series of detergent baths consisting of distilled water for 8 h, Triton X-100 for 12 h, and sodium deoxycholate for 24 hours according to the Sondell protocol. All acellularization steps were performed at room temperature. Then decellularized scaffolds were evaluated histologically and mechanically. Results: The results of tissue evaluations showed that decellularization of scaffolds were done completely, this was demonstrated by hematoxylin and eosin staining and DAPI staining. Also the specialized tissue evaluations by picro-fuchsin staining and evaluation the scaffolds by scanning electron microscopy (SEM) micrographs showed that the collagen and elastin strands are relatively preserved in the extracellular matrix in comparison with control groups. As well as mechanical examination of scaffolds in tensile test showed that extracellular matrix of scaffolds was relatively preserved the main components of tissue compared to control group and scaffolds have good mechanical resistance quality for use in tissue engineering. Conclusion: The results of the present study showed that decellularized scaffolds that prepared with Sondell decellularization method by preserving the main components of the tissue can be a good platform for investigating cellular behaviors.http://tumj.tums.ac.ir/article-1-9638-en.htmlextracellular matrixperipheral nervesregenerationsciatic nervetissue engineeringtissue scaffolds |
| spellingShingle | Arash Abdolmaleki Mohammad-Bagher Ghayour Saber Zahri Asadollah Asadi Morteza Behnam-Rassouli Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regeneration Tehran University Medical Journal extracellular matrix peripheral nerves regeneration sciatic nerve tissue engineering tissue scaffolds |
| title | Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regeneration |
| title_full | Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regeneration |
| title_fullStr | Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regeneration |
| title_full_unstemmed | Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regeneration |
| title_short | Preparation of acellular sciatic nerve scaffold and it’s mechanical and histological properties for use in peripheral nerve regeneration |
| title_sort | preparation of acellular sciatic nerve scaffold and it s mechanical and histological properties for use in peripheral nerve regeneration |
| topic | extracellular matrix peripheral nerves regeneration sciatic nerve tissue engineering tissue scaffolds |
| url | http://tumj.tums.ac.ir/article-1-9638-en.html |
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