Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment
Abstract Background Congenital cytomegalovirus (CMV) infection (cCMV) can cause sensorineural hearing loss and neurodevelopmental disabilities in children. Oral valganciclovir (VGCV) therapy has been reported to improve long-term audiological and neurodevelopmental outcomes in patients with cCMV. Th...
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BMC
2023-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-023-07995-6 |
| _version_ | 1797946026615635968 |
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| author | Yuka Torii Ichiro Morioka Yasumasa Kakei Kazumichi Fujioka Yu Kakimoto Naoto Takahashi Tetsushi Yoshikawa Hiroyuki Moriuchi Akira Oka Yoshinori Ito |
| author_facet | Yuka Torii Ichiro Morioka Yasumasa Kakei Kazumichi Fujioka Yu Kakimoto Naoto Takahashi Tetsushi Yoshikawa Hiroyuki Moriuchi Akira Oka Yoshinori Ito |
| author_sort | Yuka Torii |
| collection | DOAJ |
| description | Abstract Background Congenital cytomegalovirus (CMV) infection (cCMV) can cause sensorineural hearing loss and neurodevelopmental disabilities in children. Oral valganciclovir (VGCV) therapy has been reported to improve long-term audiological and neurodevelopmental outcomes in patients with cCMV. The levels of CMV DNA in whole blood have been monitored in previous studies. However, quantitative methods using whole blood have not been standardized. Recently, the plasma viral load has been standardized and widely used in CMV-associated diseases. Methods CMV viral loads in whole blood and plasma were serially measured in 24 patients with a confirmatory diagnosis of cCMV during oral VGCV therapy using an in-house real-time PCR assay. Plasma samples were assayed using the Cobas 6800 system (Roche Diagnostics) in addition to an in-house assay. Results Plasma CMV viral loads were remarkably decreased at the end of therapy compared to before therapy. A significant correlation of CMV levels between whole blood and plasma was observed (Spearman’s ρ = 0.566). The levels of CMV DNA before therapy were significantly correlated with the period of decreasing the viral loads to below the detection limit, not only in whole blood (Spearman’s ρ = 0.901) but also in plasma (Spearman, ρ = 0.804). Finally, CMV viral loads between the in-house assay and commercially available standardized assay in 75 plasma samples with positive PCR results for CMV were compared; a significant correlation was observed between the results of both assays. Conclusions There was a significant correlation between the two assays (Spearman, ρ = 0.882), suggesting that CMV plasma viral loads measured by the standardized assay are widely used to monitor the levels of CMV DNA in patients with cCMV during oral VGCV therapy. |
| first_indexed | 2024-04-10T21:04:23Z |
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| id | doaj.art-8786217d5b854c68ae21ed5436325237 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-10T21:04:23Z |
| publishDate | 2023-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-8786217d5b854c68ae21ed54363252372023-01-22T12:05:46ZengBMCBMC Infectious Diseases1471-23342023-01-012311710.1186/s12879-023-07995-6Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatmentYuka Torii0Ichiro Morioka1Yasumasa Kakei2Kazumichi Fujioka3Yu Kakimoto4Naoto Takahashi5Tetsushi Yoshikawa6Hiroyuki Moriuchi7Akira Oka8Yoshinori Ito9Department of Pediatrics, Nagoya University Graduate School of MedicineDepartment of Pediatrics and Child Health, Nihon University School of MedicineClinical and Translational Research Center, Kobe University HospitalDepartment of Pediatrics, Kobe University Graduate School of MedicineDepartment of Pediatrics, The University of TokyoDepartment of Pediatrics, The University of TokyoDepartment of Pediatrics, Fujita Health University School of MedicineDepartment of Pediatrics, Nagasaki University Graduate School of Biomedical SciencesDepartment of Pediatrics, The University of TokyoDepartment of Pediatrics, Nagoya University Graduate School of MedicineAbstract Background Congenital cytomegalovirus (CMV) infection (cCMV) can cause sensorineural hearing loss and neurodevelopmental disabilities in children. Oral valganciclovir (VGCV) therapy has been reported to improve long-term audiological and neurodevelopmental outcomes in patients with cCMV. The levels of CMV DNA in whole blood have been monitored in previous studies. However, quantitative methods using whole blood have not been standardized. Recently, the plasma viral load has been standardized and widely used in CMV-associated diseases. Methods CMV viral loads in whole blood and plasma were serially measured in 24 patients with a confirmatory diagnosis of cCMV during oral VGCV therapy using an in-house real-time PCR assay. Plasma samples were assayed using the Cobas 6800 system (Roche Diagnostics) in addition to an in-house assay. Results Plasma CMV viral loads were remarkably decreased at the end of therapy compared to before therapy. A significant correlation of CMV levels between whole blood and plasma was observed (Spearman’s ρ = 0.566). The levels of CMV DNA before therapy were significantly correlated with the period of decreasing the viral loads to below the detection limit, not only in whole blood (Spearman’s ρ = 0.901) but also in plasma (Spearman, ρ = 0.804). Finally, CMV viral loads between the in-house assay and commercially available standardized assay in 75 plasma samples with positive PCR results for CMV were compared; a significant correlation was observed between the results of both assays. Conclusions There was a significant correlation between the two assays (Spearman, ρ = 0.882), suggesting that CMV plasma viral loads measured by the standardized assay are widely used to monitor the levels of CMV DNA in patients with cCMV during oral VGCV therapy.https://doi.org/10.1186/s12879-023-07995-6Congenital CMV infectionPCRPlasmaValganciclovirWhole blood |
| spellingShingle | Yuka Torii Ichiro Morioka Yasumasa Kakei Kazumichi Fujioka Yu Kakimoto Naoto Takahashi Tetsushi Yoshikawa Hiroyuki Moriuchi Akira Oka Yoshinori Ito Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment BMC Infectious Diseases Congenital CMV infection PCR Plasma Valganciclovir Whole blood |
| title | Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment |
| title_full | Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment |
| title_fullStr | Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment |
| title_full_unstemmed | Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment |
| title_short | Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment |
| title_sort | correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment |
| topic | Congenital CMV infection PCR Plasma Valganciclovir Whole blood |
| url | https://doi.org/10.1186/s12879-023-07995-6 |
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