Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier
Inflammatory bowel disease (IBD) comprises systemic inflammatory conditions primarily affecting the gastrointestinal tract, including Crohn’s disease and ulcerative colitis. This research aims to analyze the clinical symptoms and pathogenesis of a Dextran sodium sulfate (DSS)-induced canine IBD mode...
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MDPI AG
2023-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/11/2616 |
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| author | Aipeng Mao Weigang Zhao Yuhang Zhu Fantao Kong Danyang Chen Huazhe Si Chao Xu |
| author_facet | Aipeng Mao Weigang Zhao Yuhang Zhu Fantao Kong Danyang Chen Huazhe Si Chao Xu |
| author_sort | Aipeng Mao |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) comprises systemic inflammatory conditions primarily affecting the gastrointestinal tract, including Crohn’s disease and ulcerative colitis. This research aims to analyze the clinical symptoms and pathogenesis of a Dextran sodium sulfate (DSS)-induced canine IBD model and evaluate the restorative effect of ginsenoside from a pathogenesis perspective. We established the DSS-induced canine IBD model and studied the pathological mechanisms. Additionally, we examined the therapeutic effect of ginsenosides by assessing the Canine Inflammatory Bowel Disease Activity Index (CIBDAI), C-reactive protein (CRP) levels, colonic tissue morphology, protein expression, and mucosal bacterial community analysis. Our findings revealed a total ginsenoside content of 22.7% in the ginsenoside extract. Animal experiments demonstrated that dogs with IBD exhibited decreased mental state, significantly increased CIBDAI and CRP levels, disrupted colonic epithelial tissue structure, decreased expression of mucin, tight junctions, and adherens junctions, as well as reduced diversity of the colonic mucosal bacterial community. Furthermore, correlation analysis highlighted a total of 38 bacterial strains correlated with physiological indices. Significantly, ginsenoside treatment could improve these symptoms and reverse the relative abundance of some bacterial communities. In conclusion, alterations in the properties of the colonic mucus layer or the reduction in MUC2, its core component, in dogs with IBD can lead to bacterial penetration of the mucus layer and subsequent contact with intestinal epithelial cells, resulting in inflammation. Remarkably, ginsenoside intervention showcased the capacity to positively influence the relative abundance of bacteria and impact the colonic mucus layer properties, thereby offering promising prospects for IBD management and recovery. |
| first_indexed | 2024-03-09T16:36:21Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T16:36:21Z |
| publishDate | 2023-10-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-87862fd5d44040dab5aa11b928174cb92023-11-24T14:56:40ZengMDPI AGMicroorganisms2076-26072023-10-011111261610.3390/microorganisms11112616Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal BarrierAipeng Mao0Weigang Zhao1Yuhang Zhu2Fantao Kong3Danyang Chen4Huazhe Si5Chao Xu6Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Research Center for Microbial Feed Engineering of Special Animals in Jilin Province, Innovation Center for Feeding and Utilization of Special Animals in Jilin Province, Changchun 130112, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Research Center for Microbial Feed Engineering of Special Animals in Jilin Province, Innovation Center for Feeding and Utilization of Special Animals in Jilin Province, Changchun 130112, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Research Center for Microbial Feed Engineering of Special Animals in Jilin Province, Innovation Center for Feeding and Utilization of Special Animals in Jilin Province, Changchun 130112, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Research Center for Microbial Feed Engineering of Special Animals in Jilin Province, Innovation Center for Feeding and Utilization of Special Animals in Jilin Province, Changchun 130112, ChinaCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, ChinaInstitute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Research Center for Microbial Feed Engineering of Special Animals in Jilin Province, Innovation Center for Feeding and Utilization of Special Animals in Jilin Province, Changchun 130112, ChinaInflammatory bowel disease (IBD) comprises systemic inflammatory conditions primarily affecting the gastrointestinal tract, including Crohn’s disease and ulcerative colitis. This research aims to analyze the clinical symptoms and pathogenesis of a Dextran sodium sulfate (DSS)-induced canine IBD model and evaluate the restorative effect of ginsenoside from a pathogenesis perspective. We established the DSS-induced canine IBD model and studied the pathological mechanisms. Additionally, we examined the therapeutic effect of ginsenosides by assessing the Canine Inflammatory Bowel Disease Activity Index (CIBDAI), C-reactive protein (CRP) levels, colonic tissue morphology, protein expression, and mucosal bacterial community analysis. Our findings revealed a total ginsenoside content of 22.7% in the ginsenoside extract. Animal experiments demonstrated that dogs with IBD exhibited decreased mental state, significantly increased CIBDAI and CRP levels, disrupted colonic epithelial tissue structure, decreased expression of mucin, tight junctions, and adherens junctions, as well as reduced diversity of the colonic mucosal bacterial community. Furthermore, correlation analysis highlighted a total of 38 bacterial strains correlated with physiological indices. Significantly, ginsenoside treatment could improve these symptoms and reverse the relative abundance of some bacterial communities. In conclusion, alterations in the properties of the colonic mucus layer or the reduction in MUC2, its core component, in dogs with IBD can lead to bacterial penetration of the mucus layer and subsequent contact with intestinal epithelial cells, resulting in inflammation. Remarkably, ginsenoside intervention showcased the capacity to positively influence the relative abundance of bacteria and impact the colonic mucus layer properties, thereby offering promising prospects for IBD management and recovery.https://www.mdpi.com/2076-2607/11/11/2616ginsenosideinflammatory bowel diseasetight junction proteinadherens junction proteinmucosal bacterial community |
| spellingShingle | Aipeng Mao Weigang Zhao Yuhang Zhu Fantao Kong Danyang Chen Huazhe Si Chao Xu Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier Microorganisms ginsenoside inflammatory bowel disease tight junction protein adherens junction protein mucosal bacterial community |
| title | Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier |
| title_full | Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier |
| title_fullStr | Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier |
| title_full_unstemmed | Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier |
| title_short | Gut Bacterial Community Determines the Therapeutic Effect of Ginsenoside on Canine Inflammatory Bowel Disease by Modulating the Colonic Mucosal Barrier |
| title_sort | gut bacterial community determines the therapeutic effect of ginsenoside on canine inflammatory bowel disease by modulating the colonic mucosal barrier |
| topic | ginsenoside inflammatory bowel disease tight junction protein adherens junction protein mucosal bacterial community |
| url | https://www.mdpi.com/2076-2607/11/11/2616 |
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