The Pleiades are a cluster of fungal effectors that inhibit host defenses.
Biotrophic plant pathogens secrete effector proteins to manipulate the host physiology. Effectors suppress defenses and induce an environment favorable to disease development. Sequence-based prediction of effector function is impeded by their rapid evolution rate. In the maize pathogen Ustilago mayd...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-06-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1009641 |
| _version_ | 1797959165926178816 |
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| author | Fernando Navarrete Nenad Grujic Alexandra Stirnberg Indira Saado David Aleksza Michelle Gallei Hazem Adi André Alcântara Mamoona Khan Janos Bindics Marco Trujillo Armin Djamei |
| author_facet | Fernando Navarrete Nenad Grujic Alexandra Stirnberg Indira Saado David Aleksza Michelle Gallei Hazem Adi André Alcântara Mamoona Khan Janos Bindics Marco Trujillo Armin Djamei |
| author_sort | Fernando Navarrete |
| collection | DOAJ |
| description | Biotrophic plant pathogens secrete effector proteins to manipulate the host physiology. Effectors suppress defenses and induce an environment favorable to disease development. Sequence-based prediction of effector function is impeded by their rapid evolution rate. In the maize pathogen Ustilago maydis, effector-coding genes frequently organize in clusters. Here we describe the functional characterization of the pleiades, a cluster of ten effector genes, by analyzing the micro- and macroscopic phenotype of the cluster deletion and expressing these proteins in planta. Deletion of the pleiades leads to strongly impaired virulence and accumulation of reactive oxygen species (ROS) in infected tissue. Eight of the Pleiades suppress the production of ROS upon perception of pathogen associated molecular patterns (PAMPs). Although functionally redundant, the Pleiades target different host components. The paralogs Taygeta1 and Merope1 suppress ROS production in either the cytoplasm or nucleus, respectively. Merope1 targets and promotes the auto-ubiquitination activity of RFI2, a conserved family of E3 ligases that regulates the production of PAMP-triggered ROS burst in plants. |
| first_indexed | 2024-04-11T00:29:37Z |
| format | Article |
| id | doaj.art-8786c3f9252548138c73088bb049bd23 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-11T00:29:37Z |
| publishDate | 2021-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-8786c3f9252548138c73088bb049bd232023-01-08T05:30:52ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742021-06-01176e100964110.1371/journal.ppat.1009641The Pleiades are a cluster of fungal effectors that inhibit host defenses.Fernando NavarreteNenad GrujicAlexandra StirnbergIndira SaadoDavid AlekszaMichelle GalleiHazem AdiAndré AlcântaraMamoona KhanJanos BindicsMarco TrujilloArmin DjameiBiotrophic plant pathogens secrete effector proteins to manipulate the host physiology. Effectors suppress defenses and induce an environment favorable to disease development. Sequence-based prediction of effector function is impeded by their rapid evolution rate. In the maize pathogen Ustilago maydis, effector-coding genes frequently organize in clusters. Here we describe the functional characterization of the pleiades, a cluster of ten effector genes, by analyzing the micro- and macroscopic phenotype of the cluster deletion and expressing these proteins in planta. Deletion of the pleiades leads to strongly impaired virulence and accumulation of reactive oxygen species (ROS) in infected tissue. Eight of the Pleiades suppress the production of ROS upon perception of pathogen associated molecular patterns (PAMPs). Although functionally redundant, the Pleiades target different host components. The paralogs Taygeta1 and Merope1 suppress ROS production in either the cytoplasm or nucleus, respectively. Merope1 targets and promotes the auto-ubiquitination activity of RFI2, a conserved family of E3 ligases that regulates the production of PAMP-triggered ROS burst in plants.https://doi.org/10.1371/journal.ppat.1009641 |
| spellingShingle | Fernando Navarrete Nenad Grujic Alexandra Stirnberg Indira Saado David Aleksza Michelle Gallei Hazem Adi André Alcântara Mamoona Khan Janos Bindics Marco Trujillo Armin Djamei The Pleiades are a cluster of fungal effectors that inhibit host defenses. PLoS Pathogens |
| title | The Pleiades are a cluster of fungal effectors that inhibit host defenses. |
| title_full | The Pleiades are a cluster of fungal effectors that inhibit host defenses. |
| title_fullStr | The Pleiades are a cluster of fungal effectors that inhibit host defenses. |
| title_full_unstemmed | The Pleiades are a cluster of fungal effectors that inhibit host defenses. |
| title_short | The Pleiades are a cluster of fungal effectors that inhibit host defenses. |
| title_sort | pleiades are a cluster of fungal effectors that inhibit host defenses |
| url | https://doi.org/10.1371/journal.ppat.1009641 |
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