Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis)
The objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Zootecnia
2014-03-01
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| Series: | Revista Brasileira de Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300004&tlng=en |
| _version_ | 1798015386315128832 |
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| author | Ana Luiza de Oliveira Timbó Patrícia Nirlane da Costa Souza Roselaine Cristina Pereira Juliane Dornellas Nunes José Eduardo Brasil Pereira Pinto Fausto de Souza Sobrinho Lisete Chamma Davide |
| author_facet | Ana Luiza de Oliveira Timbó Patrícia Nirlane da Costa Souza Roselaine Cristina Pereira Juliane Dornellas Nunes José Eduardo Brasil Pereira Pinto Fausto de Souza Sobrinho Lisete Chamma Davide |
| author_sort | Ana Luiza de Oliveira Timbó |
| collection | DOAJ |
| description | The objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine solution for 2 or 3 hours. Ploidy level was determined by flow cytometry and confirmed by chromosome count. Fertility of the tetraploid plants was assessed through evaluation of pollen viability by staining. There was no statistical difference with regard to seedling survival and number of duplicate seedlings between the treatments. Survival rate was 8%, of which 11.45% were tetraploid genotypes. The mean viability of pollen grains from the evaluated tetraploid plants ranged from 51.75 to 55.50%. So, the tetraploids plants obtained from genotypes of a bred population of ruzigrass produce fertile pollen, in percentages that render crosses viable in genetic breeding programs. |
| first_indexed | 2024-04-11T15:33:43Z |
| format | Article |
| id | doaj.art-87891e1c653745fd9469a06d7541ce5d |
| institution | Directory Open Access Journal |
| issn | 1806-9290 |
| language | English |
| last_indexed | 2024-04-11T15:33:43Z |
| publishDate | 2014-03-01 |
| publisher | Sociedade Brasileira de Zootecnia |
| record_format | Article |
| series | Revista Brasileira de Zootecnia |
| spelling | doaj.art-87891e1c653745fd9469a06d7541ce5d2022-12-22T04:16:05ZengSociedade Brasileira de ZootecniaRevista Brasileira de Zootecnia1806-92902014-03-01433127131Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis)Ana Luiza de Oliveira Timbó0Patrícia Nirlane da Costa Souza1Roselaine Cristina Pereira2Juliane Dornellas Nunes3José Eduardo Brasil Pereira Pinto4Fausto de Souza Sobrinho5Lisete Chamma Davide6Universidade Federal de LavrasUniversidade Federal de LavrasUniversidade Federal de LavrasEmbrapa Gado de LeiteUniversidade Federal de LavrasEmbrapa Gado de LeiteUniversidade Federal de LavrasThe objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine solution for 2 or 3 hours. Ploidy level was determined by flow cytometry and confirmed by chromosome count. Fertility of the tetraploid plants was assessed through evaluation of pollen viability by staining. There was no statistical difference with regard to seedling survival and number of duplicate seedlings between the treatments. Survival rate was 8%, of which 11.45% were tetraploid genotypes. The mean viability of pollen grains from the evaluated tetraploid plants ranged from 51.75 to 55.50%. So, the tetraploids plants obtained from genotypes of a bred population of ruzigrass produce fertile pollen, in percentages that render crosses viable in genetic breeding programs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300004&tlng=enDNA contentforage plant breedingnumber of chromosomespolyploidy inductionpollen viability |
| spellingShingle | Ana Luiza de Oliveira Timbó Patrícia Nirlane da Costa Souza Roselaine Cristina Pereira Juliane Dornellas Nunes José Eduardo Brasil Pereira Pinto Fausto de Souza Sobrinho Lisete Chamma Davide Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) Revista Brasileira de Zootecnia DNA content forage plant breeding number of chromosomes polyploidy induction pollen viability |
| title | Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) |
| title_full | Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) |
| title_fullStr | Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) |
| title_full_unstemmed | Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) |
| title_short | Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis) |
| title_sort | obtaining tetraploid plants of ruzigrass brachiaria ruziziensis |
| topic | DNA content forage plant breeding number of chromosomes polyploidy induction pollen viability |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300004&tlng=en |
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