Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba)
Enzymatic hydrolysis preparation process of angiotensin-I converting enzyme (ACE) inhibitory peptides from Antarctic krill (Euphausia superba) was optimized and its stability was evaluated. Using the degreased Antarctic krill meal as the substrate and the ACE inhibition rate of enzymatic hydrolysate...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-11-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020174 |
| _version_ | 1827784200831369216 |
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| author | Shuang LI Xiaofang LIU Fuhou LI Weixia WANG Kailiang LENG Yating LI Xiangrong GUO |
| author_facet | Shuang LI Xiaofang LIU Fuhou LI Weixia WANG Kailiang LENG Yating LI Xiangrong GUO |
| author_sort | Shuang LI |
| collection | DOAJ |
| description | Enzymatic hydrolysis preparation process of angiotensin-I converting enzyme (ACE) inhibitory peptides from Antarctic krill (Euphausia superba) was optimized and its stability was evaluated. Using the degreased Antarctic krill meal as the substrate and the ACE inhibition rate of enzymatic hydrolysates as the evaluation index, alkaline protease was selected as the optimal protease for preparing ACE inhibitory peptides from Antarctic krill among six types of proteases. The optimum enzymatic hydrolysis conditions were determined through single factor and response surface experiments, which were finally confirmed as follows: Enzymatic hydrolysis time of 3.4 h, material liquid ratio of 1:7 (g/mL) and enzyme dosage of 1.6%. Under these conditions, the ACE inhibition rate of enzymatic hydrolysates was 74.37%±0.87%. The obtained ACE inhibitory peptides exhibited good thermal stability at temperatures ranging from 20 ℃ to 100 ℃ and were relatively stable under neutral and weak alkaline conditions. The ACE inhibitory activity of the peptides decreased significantly under pH<7.0 and pH>8.0 conditions (P<0.05). The peptides could maintain 86.96% of the original ACE inhibitory activity after simulated gastrointestinal digestion in vitro. The present research provides scientific support for the development of Antarctic krill protein-derived healthy foods and food-derived peptides-based antihypertensive drugs. |
| first_indexed | 2024-03-11T15:59:59Z |
| format | Article |
| id | doaj.art-878b18a860d64e6b9a0d335e4ecedffb |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-11T15:59:59Z |
| publishDate | 2023-11-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-878b18a860d64e6b9a0d335e4ecedffb2023-10-25T07:38:40ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-11-01442124325010.13386/j.issn1002-0306.20230201742023020174-21Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba)Shuang LI0Xiaofang LIU1Fuhou LI2Weixia WANG3Kailiang LENG4Yating LI5Xiangrong GUO6School of Ocean Food and Biological Engineering, Jiangsu Ocean University, Lianyungang 222005, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaSchool of Ocean Food and Biological Engineering, Jiangsu Ocean University, Lianyungang 222005, ChinaSchool of Ocean Food and Biological Engineering, Jiangsu Ocean University, Lianyungang 222005, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Sustainable Development of Polar Fisheries, Ministry of Agriculture and Rural Affairs, Qingdao 266071, ChinaEnzymatic hydrolysis preparation process of angiotensin-I converting enzyme (ACE) inhibitory peptides from Antarctic krill (Euphausia superba) was optimized and its stability was evaluated. Using the degreased Antarctic krill meal as the substrate and the ACE inhibition rate of enzymatic hydrolysates as the evaluation index, alkaline protease was selected as the optimal protease for preparing ACE inhibitory peptides from Antarctic krill among six types of proteases. The optimum enzymatic hydrolysis conditions were determined through single factor and response surface experiments, which were finally confirmed as follows: Enzymatic hydrolysis time of 3.4 h, material liquid ratio of 1:7 (g/mL) and enzyme dosage of 1.6%. Under these conditions, the ACE inhibition rate of enzymatic hydrolysates was 74.37%±0.87%. The obtained ACE inhibitory peptides exhibited good thermal stability at temperatures ranging from 20 ℃ to 100 ℃ and were relatively stable under neutral and weak alkaline conditions. The ACE inhibitory activity of the peptides decreased significantly under pH<7.0 and pH>8.0 conditions (P<0.05). The peptides could maintain 86.96% of the original ACE inhibitory activity after simulated gastrointestinal digestion in vitro. The present research provides scientific support for the development of Antarctic krill protein-derived healthy foods and food-derived peptides-based antihypertensive drugs.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020174antarctic krill (euphausia superba)ace inhibitory peptidesenzymatic hydrolysisprocess optimizationstability |
| spellingShingle | Shuang LI Xiaofang LIU Fuhou LI Weixia WANG Kailiang LENG Yating LI Xiangrong GUO Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba) Shipin gongye ke-ji antarctic krill (euphausia superba) ace inhibitory peptides enzymatic hydrolysis process optimization stability |
| title | Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba) |
| title_full | Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba) |
| title_fullStr | Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba) |
| title_full_unstemmed | Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba) |
| title_short | Optimization of Enzymatic Hydrolysis Preparation Process and Stability Evaluation of ACE Inhibitory Peptides from Antarctic Krill (Euphausia superba) |
| title_sort | optimization of enzymatic hydrolysis preparation process and stability evaluation of ace inhibitory peptides from antarctic krill euphausia superba |
| topic | antarctic krill (euphausia superba) ace inhibitory peptides enzymatic hydrolysis process optimization stability |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023020174 |
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