PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis
Objectives This study aims to investigate whether PD-1 expressions are abnormal in patients with TAK. Methods PD-1 expression was analyzed by flow cytometry. Serum cytokines IL-10, IL-7, IL-2, IL-15, CCL2, CCL3, and CXCL10 were detected using a cytokine cytometric bead array. Immunohistochemistry st...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2023-07-01
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| Series: | Clinical and Applied Thrombosis/Hemostasis |
| Online Access: | https://doi.org/10.1177/10760296231187896 |
| _version_ | 1797777718986670080 |
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| author | Na Gao PhD Taoao Li PhD Wei Cui BS Limin Zhao BS Jianghui Zhang PhD Lili Pan PhD |
| author_facet | Na Gao PhD Taoao Li PhD Wei Cui BS Limin Zhao BS Jianghui Zhang PhD Lili Pan PhD |
| author_sort | Na Gao PhD |
| collection | DOAJ |
| description | Objectives This study aims to investigate whether PD-1 expressions are abnormal in patients with TAK. Methods PD-1 expression was analyzed by flow cytometry. Serum cytokines IL-10, IL-7, IL-2, IL-15, CCL2, CCL3, and CXCL10 were detected using a cytokine cytometric bead array. Immunohistochemistry staining analysis was used to test PD-1 and programmed death-ligand 1 (PD-L1) expression in the aorta of three patients with TAK and three patients with atherosclerosis as controls. Results The mean fluorescence intensity of PD-1 in CD4 + PD-1 + cells was decreased in patients with TAK and the frequency of CD4 + Foxp3 − PD-1 + cells among CD4 + T cells was also decreased in peripheral blood relative to healthy controls ( P < .05). The percentage of CD4 + CD25 + Foxp3 + PD-1 + cells in the CD4 + CD25 + T cell population was lower in patients with TAK than in healthy control and was lower in active TAK group ( P < .05). Comparing PD-1 and PDL-1 expression in aorta tissue showed that patients with TAK tended to have lower levels than patients with atherosclerosis, but the difference was not significant ( P > .05). Patients with TAK had higher serum levels of IL-10, IL-7, CCL2, and CCL3 ( P < .05). Conclusions Abnormal expression of PD-1 in serum and aorta tissue of patients with TAK may contribute to TAK pathogenesis. Key points PD-1 expression in both peripheral blood and aorta tissue of TAK patients decreased relative to healthy controls, indicating that PD-1 might be involved in TAK pathogenesis. |
| first_indexed | 2024-03-12T23:08:44Z |
| format | Article |
| id | doaj.art-878bcede14234368a3daa4b3fa9b3263 |
| institution | Directory Open Access Journal |
| issn | 1938-2723 |
| language | English |
| last_indexed | 2024-03-12T23:08:44Z |
| publishDate | 2023-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Clinical and Applied Thrombosis/Hemostasis |
| spelling | doaj.art-878bcede14234368a3daa4b3fa9b32632023-07-18T09:34:30ZengSAGE PublishingClinical and Applied Thrombosis/Hemostasis1938-27232023-07-012910.1177/10760296231187896PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's ArteritisNa Gao PhDTaoao Li PhDWei Cui BSLimin Zhao BSJianghui Zhang PhDLili Pan PhDObjectives This study aims to investigate whether PD-1 expressions are abnormal in patients with TAK. Methods PD-1 expression was analyzed by flow cytometry. Serum cytokines IL-10, IL-7, IL-2, IL-15, CCL2, CCL3, and CXCL10 were detected using a cytokine cytometric bead array. Immunohistochemistry staining analysis was used to test PD-1 and programmed death-ligand 1 (PD-L1) expression in the aorta of three patients with TAK and three patients with atherosclerosis as controls. Results The mean fluorescence intensity of PD-1 in CD4 + PD-1 + cells was decreased in patients with TAK and the frequency of CD4 + Foxp3 − PD-1 + cells among CD4 + T cells was also decreased in peripheral blood relative to healthy controls ( P < .05). The percentage of CD4 + CD25 + Foxp3 + PD-1 + cells in the CD4 + CD25 + T cell population was lower in patients with TAK than in healthy control and was lower in active TAK group ( P < .05). Comparing PD-1 and PDL-1 expression in aorta tissue showed that patients with TAK tended to have lower levels than patients with atherosclerosis, but the difference was not significant ( P > .05). Patients with TAK had higher serum levels of IL-10, IL-7, CCL2, and CCL3 ( P < .05). Conclusions Abnormal expression of PD-1 in serum and aorta tissue of patients with TAK may contribute to TAK pathogenesis. Key points PD-1 expression in both peripheral blood and aorta tissue of TAK patients decreased relative to healthy controls, indicating that PD-1 might be involved in TAK pathogenesis.https://doi.org/10.1177/10760296231187896 |
| spellingShingle | Na Gao PhD Taoao Li PhD Wei Cui BS Limin Zhao BS Jianghui Zhang PhD Lili Pan PhD PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis Clinical and Applied Thrombosis/Hemostasis |
| title | PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis |
| title_full | PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis |
| title_fullStr | PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis |
| title_full_unstemmed | PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis |
| title_short | PD-1 Deficiency in Regulatory T Cells May be Involved in the Pathogenesis of Takayasu's Arteritis |
| title_sort | pd 1 deficiency in regulatory t cells may be involved in the pathogenesis of takayasu s arteritis |
| url | https://doi.org/10.1177/10760296231187896 |
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