Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats
Abstract Background Impaired wound healing is a debilitating complication of diabetes that leads to significant morbidity, particularly foot ulcers. The risk of developing diabetic foot ulcers for diabetic patients is 15% over their lifetime and approximately 85% of limb amputations is caused by non...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-01-01
|
| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2427-y |
| _version_ | 1818049346964291584 |
|---|---|
| author | Woan Sean Tan Palanisamy Arulselvan Shiow-Fern Ng Che Norma Mat Taib Murni Nazira Sarian Sharida Fakurazi |
| author_facet | Woan Sean Tan Palanisamy Arulselvan Shiow-Fern Ng Che Norma Mat Taib Murni Nazira Sarian Sharida Fakurazi |
| author_sort | Woan Sean Tan |
| collection | DOAJ |
| description | Abstract Background Impaired wound healing is a debilitating complication of diabetes that leads to significant morbidity, particularly foot ulcers. The risk of developing diabetic foot ulcers for diabetic patients is 15% over their lifetime and approximately 85% of limb amputations is caused by non–healing ulcers. Unhealed, gangrenous wounds destroy the structural integrity of the skin, which acts as a protective barrier that prevents the invasion of external noxious agents into the body. Vicenin-2 (VCN-2) has been reported to contain prospective anti-oxidant and anti-inflammatory properties that enhance cell proliferation and migration. Sodium Alginate (SA) is a natural polysaccharide that possesses gel forming properties and has biodegradable and biocompatible characteristics. Therefore, the objective of this study is to evaluate the effect of SA wound dressings containing VCN-2 on diabetic wounds. Methods Wounds were inflicted in type-1 diabetic-streptozotocin (STZ) induced male Sprague Dawley rats. Subsequently, relevant groups were topically treated with the indicated concentrations (12.5, 25 and 50 μM) of VCN-2 hydrocolloid film over the study duration (14 days). The control group was treated with vehicle dressing (blank or allantoin). Wounded tissues and blood serum were collected on 0, 7 and 14 days prior to sacrifice. Appropriate wound assessments such as histological tests, nitric oxide assays, enzyme-linked immunosorbent assays (ELISA) and immunoblotting assays were conducted to confirm wound healing efficacy in the in vivo model. One-way Analysis of Variance (ANOVA) was used for statistical analysis. Results Results showed that hydrocolloid film was recapitulated with VCN-2 enhanced diabetic wound healing in a dose-dependent manner. VCN-2 reduced pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α), mediators (iNOS and COX-2), and nitric oxide (NO) via the NF-κB pathway. Data suggests that the VCN-2 film facilitated healing in hyperglycemic conditions by releasing growth factors such as (VEGF and TGF-β) to enhance cell proliferation, migration, and wound contraction via the VEGF and TGF-β mechanism pathways. Conclusions This study’s findings suggest that VCN-2 may possess wound healing potential since topical treatment with VCN-2 hydrocolloid films effectively enhanced wound healing in hyperglycemic conditions. |
| first_indexed | 2024-12-10T10:36:08Z |
| format | Article |
| id | doaj.art-878f51c1a42c4bcb93adda39a1cd36c5 |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-10T10:36:08Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-878f51c1a42c4bcb93adda39a1cd36c52022-12-22T01:52:26ZengBMCBMC Complementary and Alternative Medicine1472-68822019-01-0119111610.1186/s12906-018-2427-yImprovement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley ratsWoan Sean Tan0Palanisamy Arulselvan1Shiow-Fern Ng2Che Norma Mat Taib3Murni Nazira Sarian4Sharida Fakurazi5Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra MalaysiaLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra MalaysiaCentre of Drug Delivery Research, Faculty of Pharmacy, Universiti Kebangsaan MalaysiaDepartment of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra MalaysiaLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra MalaysiaLaboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra MalaysiaAbstract Background Impaired wound healing is a debilitating complication of diabetes that leads to significant morbidity, particularly foot ulcers. The risk of developing diabetic foot ulcers for diabetic patients is 15% over their lifetime and approximately 85% of limb amputations is caused by non–healing ulcers. Unhealed, gangrenous wounds destroy the structural integrity of the skin, which acts as a protective barrier that prevents the invasion of external noxious agents into the body. Vicenin-2 (VCN-2) has been reported to contain prospective anti-oxidant and anti-inflammatory properties that enhance cell proliferation and migration. Sodium Alginate (SA) is a natural polysaccharide that possesses gel forming properties and has biodegradable and biocompatible characteristics. Therefore, the objective of this study is to evaluate the effect of SA wound dressings containing VCN-2 on diabetic wounds. Methods Wounds were inflicted in type-1 diabetic-streptozotocin (STZ) induced male Sprague Dawley rats. Subsequently, relevant groups were topically treated with the indicated concentrations (12.5, 25 and 50 μM) of VCN-2 hydrocolloid film over the study duration (14 days). The control group was treated with vehicle dressing (blank or allantoin). Wounded tissues and blood serum were collected on 0, 7 and 14 days prior to sacrifice. Appropriate wound assessments such as histological tests, nitric oxide assays, enzyme-linked immunosorbent assays (ELISA) and immunoblotting assays were conducted to confirm wound healing efficacy in the in vivo model. One-way Analysis of Variance (ANOVA) was used for statistical analysis. Results Results showed that hydrocolloid film was recapitulated with VCN-2 enhanced diabetic wound healing in a dose-dependent manner. VCN-2 reduced pro-inflammatory cytokines (IL-1β, IL-6 and TNF-α), mediators (iNOS and COX-2), and nitric oxide (NO) via the NF-κB pathway. Data suggests that the VCN-2 film facilitated healing in hyperglycemic conditions by releasing growth factors such as (VEGF and TGF-β) to enhance cell proliferation, migration, and wound contraction via the VEGF and TGF-β mechanism pathways. Conclusions This study’s findings suggest that VCN-2 may possess wound healing potential since topical treatment with VCN-2 hydrocolloid films effectively enhanced wound healing in hyperglycemic conditions.http://link.springer.com/article/10.1186/s12906-018-2427-yVicenin-2Sodium alginateHydrocolloid filmDiabetic wound |
| spellingShingle | Woan Sean Tan Palanisamy Arulselvan Shiow-Fern Ng Che Norma Mat Taib Murni Nazira Sarian Sharida Fakurazi Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats BMC Complementary and Alternative Medicine Vicenin-2 Sodium alginate Hydrocolloid film Diabetic wound |
| title | Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats |
| title_full | Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats |
| title_fullStr | Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats |
| title_full_unstemmed | Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats |
| title_short | Improvement of diabetic wound healing by topical application of Vicenin-2 hydrocolloid film on Sprague Dawley rats |
| title_sort | improvement of diabetic wound healing by topical application of vicenin 2 hydrocolloid film on sprague dawley rats |
| topic | Vicenin-2 Sodium alginate Hydrocolloid film Diabetic wound |
| url | http://link.springer.com/article/10.1186/s12906-018-2427-y |
| work_keys_str_mv | AT woanseantan improvementofdiabeticwoundhealingbytopicalapplicationofvicenin2hydrocolloidfilmonspraguedawleyrats AT palanisamyarulselvan improvementofdiabeticwoundhealingbytopicalapplicationofvicenin2hydrocolloidfilmonspraguedawleyrats AT shiowfernng improvementofdiabeticwoundhealingbytopicalapplicationofvicenin2hydrocolloidfilmonspraguedawleyrats AT chenormamattaib improvementofdiabeticwoundhealingbytopicalapplicationofvicenin2hydrocolloidfilmonspraguedawleyrats AT murninazirasarian improvementofdiabeticwoundhealingbytopicalapplicationofvicenin2hydrocolloidfilmonspraguedawleyrats AT sharidafakurazi improvementofdiabeticwoundhealingbytopicalapplicationofvicenin2hydrocolloidfilmonspraguedawleyrats |