Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of Endometriosis
<b>Backgroud:</b> Endometriosis remains a diagnostic challenge, both clinically and economically, affecting 6% to 15% of women of child-bearing potential. We have attempted to determine whether testing serum concentrations and activity of arginase isoenzymes could be useful for the non-i...
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MDPI AG
2024-03-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/13/5/1489 |
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| author | Maciej Pliszkiewicz Malgorzata Czystowska-Kuzmicz Karolina Soroczynska Bogumił Paweł Siekierski Krzysztof Safranow |
| author_facet | Maciej Pliszkiewicz Malgorzata Czystowska-Kuzmicz Karolina Soroczynska Bogumił Paweł Siekierski Krzysztof Safranow |
| author_sort | Maciej Pliszkiewicz |
| collection | DOAJ |
| description | <b>Backgroud:</b> Endometriosis remains a diagnostic challenge, both clinically and economically, affecting 6% to 15% of women of child-bearing potential. We have attempted to determine whether testing serum concentrations and activity of arginase isoenzymes could be useful for the non-invasive diagnosis of endometriosis. <b>Methods:</b> This study involved 180 women (105 endometriosis subjects—study group B; 22 subjects with other benign gynaecological conditions—control group 1—K1, both undergoing surgery; and 53 healthy subjects without features of endometriosis—control group 2—K2). <b>Results:</b> Preoperative and postoperative arginase-1 (Arg-1) concentrations were significantly higher in patients, as compared with the control groups K1 (<i>p</i> < 0.0001 and <i>p</i> = 0.0005, respectively) and K2 (both <i>p</i> < 0.0001). Similarly, arginase activity was significantly higher in patients than in the control group K1 before surgery and higher than in both control groups after surgery. No significant differences in either Arg-1 concentrations or arginase activity were noted between the operated control group K1 and the non-operated control group K2. A significant postoperative decrease in Arg-1 concentration was observed within both patient (<i>p</i> < 0.0001) and control group K1 (<i>p</i> = 0.0043). Diagnostic performance was assessed using the receiver operating characteristic (ROC) method. The threshold for differentiation between endometriosis patients and healthy non-operated controls was 42.3 ng/mL, with a sensitivity of 90% and specificity of 81%. For differentiation of patients and operated controls with benign gynaecological conditions, the threshold was 78.4 ng/mL, with a sensitivity of 61% and specificity of 95%. <b>Conclusions:</b> We, therefore, conclude that Arg-1 serum concentrations and arginase activity could be considered potential biomarkers for endometriosis but require further studies on larger cohorts of patients. |
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| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-04-25T00:26:44Z |
| publishDate | 2024-03-01 |
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| series | Journal of Clinical Medicine |
| spelling | doaj.art-8795e415a6244e5b9bd604cdc0d2825d2024-03-12T16:48:39ZengMDPI AGJournal of Clinical Medicine2077-03832024-03-01135148910.3390/jcm13051489Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of EndometriosisMaciej Pliszkiewicz0Malgorzata Czystowska-Kuzmicz1Karolina Soroczynska2Bogumił Paweł Siekierski3Krzysztof Safranow4Department of Gynaecology, Medicover Hospital, 02-972 Warsaw, PolandChair and Department of Biochemistry, Medical University of Warsaw, Banacha 1 St., 02-097 Warsaw, PolandChair and Department of Biochemistry, Medical University of Warsaw, Banacha 1 St., 02-097 Warsaw, PolandDepartment of Gynaecology, Medicover Hospital, 02-972 Warsaw, PolandDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in Szczecin, Powstańców Wlkp. 72, 70-111 Szczecin, Poland<b>Backgroud:</b> Endometriosis remains a diagnostic challenge, both clinically and economically, affecting 6% to 15% of women of child-bearing potential. We have attempted to determine whether testing serum concentrations and activity of arginase isoenzymes could be useful for the non-invasive diagnosis of endometriosis. <b>Methods:</b> This study involved 180 women (105 endometriosis subjects—study group B; 22 subjects with other benign gynaecological conditions—control group 1—K1, both undergoing surgery; and 53 healthy subjects without features of endometriosis—control group 2—K2). <b>Results:</b> Preoperative and postoperative arginase-1 (Arg-1) concentrations were significantly higher in patients, as compared with the control groups K1 (<i>p</i> < 0.0001 and <i>p</i> = 0.0005, respectively) and K2 (both <i>p</i> < 0.0001). Similarly, arginase activity was significantly higher in patients than in the control group K1 before surgery and higher than in both control groups after surgery. No significant differences in either Arg-1 concentrations or arginase activity were noted between the operated control group K1 and the non-operated control group K2. A significant postoperative decrease in Arg-1 concentration was observed within both patient (<i>p</i> < 0.0001) and control group K1 (<i>p</i> = 0.0043). Diagnostic performance was assessed using the receiver operating characteristic (ROC) method. The threshold for differentiation between endometriosis patients and healthy non-operated controls was 42.3 ng/mL, with a sensitivity of 90% and specificity of 81%. For differentiation of patients and operated controls with benign gynaecological conditions, the threshold was 78.4 ng/mL, with a sensitivity of 61% and specificity of 95%. <b>Conclusions:</b> We, therefore, conclude that Arg-1 serum concentrations and arginase activity could be considered potential biomarkers for endometriosis but require further studies on larger cohorts of patients.https://www.mdpi.com/2077-0383/13/5/1489endometriosisendometriosis diagnosisarginasenon-invasivebiomarkerimmunosuppression |
| spellingShingle | Maciej Pliszkiewicz Malgorzata Czystowska-Kuzmicz Karolina Soroczynska Bogumił Paweł Siekierski Krzysztof Safranow Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of Endometriosis Journal of Clinical Medicine endometriosis endometriosis diagnosis arginase non-invasive biomarker immunosuppression |
| title | Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of Endometriosis |
| title_full | Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of Endometriosis |
| title_fullStr | Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of Endometriosis |
| title_full_unstemmed | Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of Endometriosis |
| title_short | Determination of Serum Arginase-1 Concentrations and Serum Arginase Activity for the Non-Invasive Diagnosis of Endometriosis |
| title_sort | determination of serum arginase 1 concentrations and serum arginase activity for the non invasive diagnosis of endometriosis |
| topic | endometriosis endometriosis diagnosis arginase non-invasive biomarker immunosuppression |
| url | https://www.mdpi.com/2077-0383/13/5/1489 |
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