Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma
<p>Abstract</p> <p>Background</p> <p>Osteosarcoma (OSA) represents the most common canine primary bone tumour. Despite several pathways have been investigated so far, few molecules have been identified as prognostic tools or potential therapeutic targets, and there is s...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-06-01
|
| Series: | BMC Veterinary Research |
| Online Access: | http://www.biomedcentral.com/1746-6148/8/78 |
| _version_ | 1811275971476586496 |
|---|---|
| author | Bongiovanni Laura Mazzocchetti Francesca Malatesta Daniela Romanucci Mariarita Ciccarelli Andrea Buracco Paolo De Maria Raffaella Palmieri Chiara Martano Marina Morello Emanuela Maniscalco Lorella Salda Leonardo |
| author_facet | Bongiovanni Laura Mazzocchetti Francesca Malatesta Daniela Romanucci Mariarita Ciccarelli Andrea Buracco Paolo De Maria Raffaella Palmieri Chiara Martano Marina Morello Emanuela Maniscalco Lorella Salda Leonardo |
| author_sort | Bongiovanni Laura |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Osteosarcoma (OSA) represents the most common canine primary bone tumour. Despite several pathways have been investigated so far, few molecules have been identified as prognostic tools or potential therapeutic targets, and there is still the need to find out molecular pathways with specific influence over OSA progression to facilitate earlier prognosis and treatment.</p> <p>Aims of the present study were to evaluate the immunohistochemical pattern and levels of expression of a panel of molecules (survivin, β-catenin, caspase 3 -inactive and active forms- and p53) involved in cell cycle and apoptosis regulation in canine OSA samples, known to be of interest in the study also of human OSA, and to detect specific relations among them and with histological tumour grade, disease free interval (DFI) and overall survival (OS).</p> <p>Results</p> <p>Nuclear β-catenin immunostaining was detected in normal osteoblasts adjacent to the tumour, and in 47% of the cases. Cytoplasmic and/or membranous immunostaining were also observed. Nuclear survivin and p53 positive cells were found in all cases. Moderate/high cytoplasmic β-catenin expression (≥10% positive cells) was significantly associated with the development of metastasis (P = 0.014); moderate/high nuclear p53 expression (≥10% positive cells) was significantly associated with moderate/high histological grade (P = 0.017) and shorter OS (P = 0.049). Moderate/high nuclear survivin expression (≥15% positive cells) showed a tendency toward a longer OS (P = 0,088).</p> <p>Conclusions</p> <p>The present results confirmed p53 as negative prognostic marker, while suggested survivin as a potential positive prognostic indicator, rather than indicative of a poor prognosis. The detection of nuclear β-catenin immunostaining in normal osteoblasts and the absent/low expression in most of the OSAs, suggested that this pathway could not play a major role in oncogenic transformation of canine osteoblasts. Further studies are needed to confirm these hypotheses.</p> |
| first_indexed | 2024-04-12T23:48:14Z |
| format | Article |
| id | doaj.art-879d33dfea3a4b67ae4c08c2a1b56b48 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-04-12T23:48:14Z |
| publishDate | 2012-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-879d33dfea3a4b67ae4c08c2a1b56b482022-12-22T03:11:48ZengBMCBMC Veterinary Research1746-61482012-06-01817810.1186/1746-6148-8-78Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcomaBongiovanni LauraMazzocchetti FrancescaMalatesta DanielaRomanucci MariaritaCiccarelli AndreaBuracco PaoloDe Maria RaffaellaPalmieri ChiaraMartano MarinaMorello EmanuelaManiscalco LorellaSalda Leonardo<p>Abstract</p> <p>Background</p> <p>Osteosarcoma (OSA) represents the most common canine primary bone tumour. Despite several pathways have been investigated so far, few molecules have been identified as prognostic tools or potential therapeutic targets, and there is still the need to find out molecular pathways with specific influence over OSA progression to facilitate earlier prognosis and treatment.</p> <p>Aims of the present study were to evaluate the immunohistochemical pattern and levels of expression of a panel of molecules (survivin, β-catenin, caspase 3 -inactive and active forms- and p53) involved in cell cycle and apoptosis regulation in canine OSA samples, known to be of interest in the study also of human OSA, and to detect specific relations among them and with histological tumour grade, disease free interval (DFI) and overall survival (OS).</p> <p>Results</p> <p>Nuclear β-catenin immunostaining was detected in normal osteoblasts adjacent to the tumour, and in 47% of the cases. Cytoplasmic and/or membranous immunostaining were also observed. Nuclear survivin and p53 positive cells were found in all cases. Moderate/high cytoplasmic β-catenin expression (≥10% positive cells) was significantly associated with the development of metastasis (P = 0.014); moderate/high nuclear p53 expression (≥10% positive cells) was significantly associated with moderate/high histological grade (P = 0.017) and shorter OS (P = 0.049). Moderate/high nuclear survivin expression (≥15% positive cells) showed a tendency toward a longer OS (P = 0,088).</p> <p>Conclusions</p> <p>The present results confirmed p53 as negative prognostic marker, while suggested survivin as a potential positive prognostic indicator, rather than indicative of a poor prognosis. The detection of nuclear β-catenin immunostaining in normal osteoblasts and the absent/low expression in most of the OSAs, suggested that this pathway could not play a major role in oncogenic transformation of canine osteoblasts. Further studies are needed to confirm these hypotheses.</p>http://www.biomedcentral.com/1746-6148/8/78 |
| spellingShingle | Bongiovanni Laura Mazzocchetti Francesca Malatesta Daniela Romanucci Mariarita Ciccarelli Andrea Buracco Paolo De Maria Raffaella Palmieri Chiara Martano Marina Morello Emanuela Maniscalco Lorella Salda Leonardo Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma BMC Veterinary Research |
| title | Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma |
| title_full | Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma |
| title_fullStr | Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma |
| title_full_unstemmed | Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma |
| title_short | Immunohistochemical investigation of cell cycle and apoptosis regulators (Survivin, β-Catenin, P53, Caspase 3) in canine appendicular osteosarcoma |
| title_sort | immunohistochemical investigation of cell cycle and apoptosis regulators survivin β catenin p53 caspase 3 in canine appendicular osteosarcoma |
| url | http://www.biomedcentral.com/1746-6148/8/78 |
| work_keys_str_mv | AT bongiovannilaura immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT mazzocchettifrancesca immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT malatestadaniela immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT romanuccimariarita immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT ciccarelliandrea immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT buraccopaolo immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT demariaraffaella immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT palmierichiara immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT martanomarina immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT morelloemanuela immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT maniscalcolorella immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma AT saldaleonardo immunohistochemicalinvestigationofcellcycleandapoptosisregulatorssurvivinbcateninp53caspase3incanineappendicularosteosarcoma |