Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran
Inter-Simple Sequence Repeat (ISSR) molecular marker for the isolation of five races of honey bee Apis mellifera from Khuzestan, Kurdistan, Markazi, Fars and Isfahan provinces was studied. DNA was extracted from the worker bees. The quality and quantity of extracted DNA were measured. Values obtain...
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| Format: | Article |
| Language: | English |
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Plovdiv University Press
2014-08-01
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| Series: | Journal of BioScience and Biotechnology |
| Subjects: | |
| Online Access: | http://www.jbb.uni-plovdiv.bg/documents/27807/352486/jbb_2014-3(2)-pages_127-131.pdf |
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| author | HOSSEIN SHOUHANI ABOUFAZEL DOUSTI ROUHOLLAH RADJABI MOJTABA ZAREI |
| author_facet | HOSSEIN SHOUHANI ABOUFAZEL DOUSTI ROUHOLLAH RADJABI MOJTABA ZAREI |
| author_sort | HOSSEIN SHOUHANI |
| collection | DOAJ |
| description | Inter-Simple Sequence Repeat (ISSR) molecular marker for the isolation of five races of honey bee Apis mellifera from Khuzestan, Kurdistan, Markazi, Fars and Isfahan provinces was studied. DNA was extracted from the worker bees. The quality and quantity of extracted DNA were measured. Values obtained from the bands on agarose gel (1.5%) were scored and analyzed. Results showed that the bands are in the range between 250 and 1000 bp. Maximum number of bands were recorded for primer 1. Cluster analysis of races placed them in two main groups. In the first group were Kurdistan and Khuzestan. The second group includes Markazi, Fars and Isfahan, but this group has shown no relationship between the populations. It seems that ISSR molecular marker could separate different races of honey bee. |
| first_indexed | 2024-12-19T02:49:19Z |
| format | Article |
| id | doaj.art-879f58e39e394b8eae6a3e9078bf874a |
| institution | Directory Open Access Journal |
| issn | 1314-6238 1314-6246 |
| language | English |
| last_indexed | 2024-12-19T02:49:19Z |
| publishDate | 2014-08-01 |
| publisher | Plovdiv University Press |
| record_format | Article |
| series | Journal of BioScience and Biotechnology |
| spelling | doaj.art-879f58e39e394b8eae6a3e9078bf874a2022-12-21T20:38:45ZengPlovdiv University PressJournal of BioScience and Biotechnology1314-62381314-62462014-08-0132127131Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of IranHOSSEIN SHOUHANI0ABOUFAZEL DOUSTI1ROUHOLLAH RADJABI2MOJTABA ZAREI3Entomology Department, Agriculture College, Islamic Azad University, Jahrom Branch, Jahrom, Iran. Entomology Department, Agriculture College, Islamic Azad University, Jahrom Branch, Jahrom, Iran. Plant Protection Department, Agriculture College, Dezful Branch, Islamic Azad University, Dezful, Iran.Entomology Department, Agriculture College, Islamic Azad University, Arak Branch, Arak, Iran.Inter-Simple Sequence Repeat (ISSR) molecular marker for the isolation of five races of honey bee Apis mellifera from Khuzestan, Kurdistan, Markazi, Fars and Isfahan provinces was studied. DNA was extracted from the worker bees. The quality and quantity of extracted DNA were measured. Values obtained from the bands on agarose gel (1.5%) were scored and analyzed. Results showed that the bands are in the range between 250 and 1000 bp. Maximum number of bands were recorded for primer 1. Cluster analysis of races placed them in two main groups. In the first group were Kurdistan and Khuzestan. The second group includes Markazi, Fars and Isfahan, but this group has shown no relationship between the populations. It seems that ISSR molecular marker could separate different races of honey bee.http://www.jbb.uni-plovdiv.bg/documents/27807/352486/jbb_2014-3(2)-pages_127-131.pdfmolecular markerhoney beeDNAgenetic diversity |
| spellingShingle | HOSSEIN SHOUHANI ABOUFAZEL DOUSTI ROUHOLLAH RADJABI MOJTABA ZAREI Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran Journal of BioScience and Biotechnology molecular marker honey bee DNA genetic diversity |
| title | Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran |
| title_full | Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran |
| title_fullStr | Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran |
| title_full_unstemmed | Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran |
| title_short | Application of ISSR to study the genetic diversity of honeybee (Apis mellifera L.) populations in some areas of Iran |
| title_sort | application of issr to study the genetic diversity of honeybee apis mellifera l populations in some areas of iran |
| topic | molecular marker honey bee DNA genetic diversity |
| url | http://www.jbb.uni-plovdiv.bg/documents/27807/352486/jbb_2014-3(2)-pages_127-131.pdf |
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