Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro study
An in vitro gas production study was conducted to test the effect of papaya leaf (PL) at different concentrations of 0 (CON), 10% (Low PL: LPL), 15% (Medium PL: MPL) and 25% (High PL: HPL) of replacement with alfalfa hay (AH) in substrate (50% AH + 50% concentrate) on rumen methane (CH4) production,...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2016-01-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2016.1141031 |
| _version_ | 1819090519180967936 |
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| author | Saeid Jafari Yong Meng Goh Mohamed Ali Rajion Mohammad Faseleh Jahromi Mahdi Ebrahimi |
| author_facet | Saeid Jafari Yong Meng Goh Mohamed Ali Rajion Mohammad Faseleh Jahromi Mahdi Ebrahimi |
| author_sort | Saeid Jafari |
| collection | DOAJ |
| description | An in vitro gas production study was conducted to test the effect of papaya leaf (PL) at different concentrations of 0 (CON), 10% (Low PL: LPL), 15% (Medium PL: MPL) and 25% (High PL: HPL) of replacement with alfalfa hay (AH) in substrate (50% AH + 50% concentrate) on rumen methane (CH4) production, rumen biohydrogenation (BH) of polyunsaturated fatty acid (PUFA) and rumen microbial population. PL at different concentrations were mixed with 30 ml of buffered rumen fluid and were incubated for 24 h. Methane production (ml/250 mg DM) declined at a decreasing rate (linear p = 0.03) with increasing levels of PL. The acetic/propionic ratio (1.80) was lowest (p < 0.05) for HPL compared with CON (2.04), LPL (1.95) and MPL (1.89). Supplementation of the diet with PL significantly (p < 0.05) decreased the rate of BH of C18:2n-6 (LA) and C18 PUFA after 24 h of incubation especially at the higher inclusion rate, resulting in an increased production of rumen BH intermediates such as conjugated linoleic acid (CLA), whereas saturated fatty acid (SFA) decreased in the rumen fluid after 24 h of incubation. Estimates of rumen microbial population using real-time PCR assay showed a reduction in total methanogens (p < 0.05) in MPL (−12%) and HPL (−16%) and increase in total bacteria population (HPL: +5%) and Butyrivibro fibrisolvens (MPL: +20; HPL: +23%) among PL treatment groups compared with the CON group. These results emphasise PL as a beneficial feed material for ruminants. |
| first_indexed | 2024-12-21T22:25:07Z |
| format | Article |
| id | doaj.art-87a953381557452086d3a2b08b5d09d1 |
| institution | Directory Open Access Journal |
| issn | 1828-051X |
| language | English |
| last_indexed | 2024-12-21T22:25:07Z |
| publishDate | 2016-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-87a953381557452086d3a2b08b5d09d12022-12-21T18:48:15ZengTaylor & Francis GroupItalian Journal of Animal Science1828-051X2016-01-0115115716510.1080/1828051X.2016.11410311141031Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro studySaeid Jafari0Yong Meng Goh1Mohamed Ali Rajion2Mohammad Faseleh Jahromi3Mahdi Ebrahimi4University Putra MalaysiaUniversity Putra MalaysiaUniversity Putra MalaysiaInstitute of Tropical Agriculture, University Putra MalaysiaUniversity Putra MalaysiaAn in vitro gas production study was conducted to test the effect of papaya leaf (PL) at different concentrations of 0 (CON), 10% (Low PL: LPL), 15% (Medium PL: MPL) and 25% (High PL: HPL) of replacement with alfalfa hay (AH) in substrate (50% AH + 50% concentrate) on rumen methane (CH4) production, rumen biohydrogenation (BH) of polyunsaturated fatty acid (PUFA) and rumen microbial population. PL at different concentrations were mixed with 30 ml of buffered rumen fluid and were incubated for 24 h. Methane production (ml/250 mg DM) declined at a decreasing rate (linear p = 0.03) with increasing levels of PL. The acetic/propionic ratio (1.80) was lowest (p < 0.05) for HPL compared with CON (2.04), LPL (1.95) and MPL (1.89). Supplementation of the diet with PL significantly (p < 0.05) decreased the rate of BH of C18:2n-6 (LA) and C18 PUFA after 24 h of incubation especially at the higher inclusion rate, resulting in an increased production of rumen BH intermediates such as conjugated linoleic acid (CLA), whereas saturated fatty acid (SFA) decreased in the rumen fluid after 24 h of incubation. Estimates of rumen microbial population using real-time PCR assay showed a reduction in total methanogens (p < 0.05) in MPL (−12%) and HPL (−16%) and increase in total bacteria population (HPL: +5%) and Butyrivibro fibrisolvens (MPL: +20; HPL: +23%) among PL treatment groups compared with the CON group. These results emphasise PL as a beneficial feed material for ruminants.http://dx.doi.org/10.1080/1828051X.2016.1141031In vitromicrobial populationpapaya leafrumen biohydrogenationrumen methanogenesis |
| spellingShingle | Saeid Jafari Yong Meng Goh Mohamed Ali Rajion Mohammad Faseleh Jahromi Mahdi Ebrahimi Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro study Italian Journal of Animal Science In vitro microbial population papaya leaf rumen biohydrogenation rumen methanogenesis |
| title | Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro study |
| title_full | Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro study |
| title_fullStr | Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro study |
| title_full_unstemmed | Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro study |
| title_short | Ruminal methanogenesis and biohydrogenation reduction potential of papaya (Carica papaya) leaf: an in vitro study |
| title_sort | ruminal methanogenesis and biohydrogenation reduction potential of papaya carica papaya leaf an in vitro study |
| topic | In vitro microbial population papaya leaf rumen biohydrogenation rumen methanogenesis |
| url | http://dx.doi.org/10.1080/1828051X.2016.1141031 |
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