A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS
Abstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bac...
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Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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Series: | Медицинская иммунология |
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Online Access: | https://www.mimmun.ru/mimmun/article/view/351 |
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author | E. V. Voloshina S. V. Zubova S. V. Prokhorenko N. I. Kosjakova I. R. Prokhorenko |
author_facet | E. V. Voloshina S. V. Zubova S. V. Prokhorenko N. I. Kosjakova I. R. Prokhorenko |
author_sort | E. V. Voloshina |
collection | DOAJ |
description | Abstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bacterial lipopolysaccharides from S. typhimurium (S-chemotype form), S. typhimurium SL1181 (R-chemotype, Re-mutant), E. coli O55:B5 (S-chemotype), and E. coli JM103 (R-chemotype, Re-mutant) were used in this study. A decreased molar ratio for lipid A-KDO in S-form of LPS from E. coli is accompanied by diminished TNFα and IL-6 expression. By the contrast, for S-form of LPS from Salmonella, a decrease in lipid A-KDO molar ratio did cause a sufficient enhancement of TNFα expression. A contribution of lipid A structure into biological activity of LPS is more significant for Re-chemotype than for S-chemotype, independently on bacterial species. |
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institution | Directory Open Access Journal |
issn | 1563-0625 2313-741X |
language | Russian |
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publishDate | 2014-07-01 |
publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
record_format | Article |
series | Медицинская иммунология |
spelling | doaj.art-87af23ca3b494916a568744bc47f730b2024-04-22T13:07:36ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0111650951410.15789/1563-0625-2009-6-509-514348A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLSE. V. Voloshina0S. V. Zubova1S. V. Prokhorenko2N. I. Kosjakova3I. R. Prokhorenko4Институт фундаментальных проблем биологии РАН, г. ПущиноИнститут фундаментальных проблем биологии РАН, г. ПущиноМосковская медицинская академия им. И.М. Сеченова, МоскваПНЦ РАН, г. ПущиноИнститут фундаментальных проблем биологии РАН, г. Пущино; Московская медицинская академия им. И.М. Сеченова, МоскваAbstract. Present study was performed to investigate the influence of polysaccharide fragment or lipid A upon induction of TNFα and IL-6 cytokines. The study was performed with human THP-1 monocytic leukemia cells that were induced to differentiate into macrophage-like cells using PMA treatment. Bacterial lipopolysaccharides from S. typhimurium (S-chemotype form), S. typhimurium SL1181 (R-chemotype, Re-mutant), E. coli O55:B5 (S-chemotype), and E. coli JM103 (R-chemotype, Re-mutant) were used in this study. A decreased molar ratio for lipid A-KDO in S-form of LPS from E. coli is accompanied by diminished TNFα and IL-6 expression. By the contrast, for S-form of LPS from Salmonella, a decrease in lipid A-KDO molar ratio did cause a sufficient enhancement of TNFα expression. A contribution of lipid A structure into biological activity of LPS is more significant for Re-chemotype than for S-chemotype, independently on bacterial species.https://www.mimmun.ru/mimmun/article/view/351lipopolysaccharidethp-1 cellstnfαil-6 |
spellingShingle | E. V. Voloshina S. V. Zubova S. V. Prokhorenko N. I. Kosjakova I. R. Prokhorenko A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS Медицинская иммунология lipopolysaccharide thp-1 cells tnfα il-6 |
title | A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS |
title_full | A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS |
title_fullStr | A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS |
title_full_unstemmed | A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS |
title_short | A COMPARISON OF DIFFERENT LIPOPOLYSACCHARIDE CHEMOTYPES FROM ESCHERICHIA COLI AND SALMONELLA UPON SYNTHESIS OF TNFα AND IL-6 BY MACROPHAGE-LIKE THP-1 CELLS |
title_sort | comparison of different lipopolysaccharide chemotypes from escherichia coli and salmonella upon synthesis of tnfα and il 6 by macrophage like thp 1 cells |
topic | lipopolysaccharide thp-1 cells tnfα il-6 |
url | https://www.mimmun.ru/mimmun/article/view/351 |
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