Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo
Extracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study,...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.1015841/full |
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| author | Weili Li Weili Li Jin Wang Jin Wang Xiaojiao Yin Huanhuan Shi Huanhuan Shi Benben Sun Mengru Ji Mengru Ji Huichen Song Huichen Song Jiachen Liu Yihao Dou Chenghong Xu Xiaohong Jiang Xiaohong Jiang Jing Li Jing Li Liang Li Liang Li Chen-Yu Zhang Chen-Yu Zhang Yujing Zhang Yujing Zhang |
| author_facet | Weili Li Weili Li Jin Wang Jin Wang Xiaojiao Yin Huanhuan Shi Huanhuan Shi Benben Sun Mengru Ji Mengru Ji Huichen Song Huichen Song Jiachen Liu Yihao Dou Chenghong Xu Xiaohong Jiang Xiaohong Jiang Jing Li Jing Li Liang Li Liang Li Chen-Yu Zhang Chen-Yu Zhang Yujing Zhang Yujing Zhang |
| author_sort | Weili Li |
| collection | DOAJ |
| description | Extracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study, a Cre-dependent CD63flag-EGFP co-expressed with mCherry protein system expressing mice was constructed, which can be used for the secretion, movement, and sorting of EVs from specific tissues in vivo. This mouse model is an ideal research tool for studying the secretion amount, target tissue, and functional molecule screening of EVs in specific tissues under different pathophysiological conditions. Moreover, it provides a new research method to clarify the mechanism of secreted EVs in the pathogenesis of the disease. |
| first_indexed | 2024-04-13T12:21:38Z |
| format | Article |
| id | doaj.art-87affcec1ff242f983f552206e4973dc |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-13T12:21:38Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-87affcec1ff242f983f552206e4973dc2022-12-22T02:47:12ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-11-011010.3389/fcell.2022.10158411015841Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivoWeili Li0Weili Li1Jin Wang2Jin Wang3Xiaojiao Yin4Huanhuan Shi5Huanhuan Shi6Benben Sun7Mengru Ji8Mengru Ji9Huichen Song10Huichen Song11Jiachen Liu12Yihao Dou13Chenghong Xu14Xiaohong Jiang15Xiaohong Jiang16Jing Li17Jing Li18Liang Li19Liang Li20Chen-Yu Zhang21Chen-Yu Zhang22Yujing Zhang23Yujing Zhang24State Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaState Key Laboratory of Pharmaceutical Biotechnology, Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, School of Life Sciences, NJU Advanced Institute of Life Sciences (NAILS), Institute of Artificial Intelligence Biomedicine, Nanjing University, Nanjing, Jiangsu, ChinaChinese Academy of Medical Sciences, Research Unit of Extracellular RNA, Nanjing, Jiangsu, ChinaExtracellular vesicles (EVs) play an important role in the communication between tissues and cells. However, it is difficult to screen and trace EVs secreted by specific tissues in vivo, which affects the functional study of EVs in certain tissues under pathophysiological conditions. In this study, a Cre-dependent CD63flag-EGFP co-expressed with mCherry protein system expressing mice was constructed, which can be used for the secretion, movement, and sorting of EVs from specific tissues in vivo. This mouse model is an ideal research tool for studying the secretion amount, target tissue, and functional molecule screening of EVs in specific tissues under different pathophysiological conditions. Moreover, it provides a new research method to clarify the mechanism of secreted EVs in the pathogenesis of the disease.https://www.frontiersin.org/articles/10.3389/fcell.2022.1015841/fullextracellular vesicles (EVs)mouse modelCD63flag-EGFPscreening and tracingtissue-specific EV |
| spellingShingle | Weili Li Weili Li Jin Wang Jin Wang Xiaojiao Yin Huanhuan Shi Huanhuan Shi Benben Sun Mengru Ji Mengru Ji Huichen Song Huichen Song Jiachen Liu Yihao Dou Chenghong Xu Xiaohong Jiang Xiaohong Jiang Jing Li Jing Li Liang Li Liang Li Chen-Yu Zhang Chen-Yu Zhang Yujing Zhang Yujing Zhang Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo Frontiers in Cell and Developmental Biology extracellular vesicles (EVs) mouse model CD63flag-EGFP screening and tracing tissue-specific EV |
| title | Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo |
| title_full | Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo |
| title_fullStr | Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo |
| title_full_unstemmed | Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo |
| title_short | Construction of a mouse model that can be used for tissue-specific EV screening and tracing in vivo |
| title_sort | construction of a mouse model that can be used for tissue specific ev screening and tracing in vivo |
| topic | extracellular vesicles (EVs) mouse model CD63flag-EGFP screening and tracing tissue-specific EV |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.1015841/full |
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