Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line
The IGF system is a family of polypeptide growth factors, which plays a significant role in the development and growth of many cells. Dysregulation of insulin-like growth factors and their pathway components has been connected with essential tumor properties, such as tumor cell proliferation, antiap...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-10-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1422-0067/16/10/23615 |
| _version_ | 1811200941317160960 |
|---|---|
| author | Marta Woźniak Kamila Duś-Szachniewicz Piotr Ziółkowski |
| author_facet | Marta Woźniak Kamila Duś-Szachniewicz Piotr Ziółkowski |
| author_sort | Marta Woźniak |
| collection | DOAJ |
| description | The IGF system is a family of polypeptide growth factors, which plays a significant role in the development and growth of many cells. Dysregulation of insulin-like growth factors and their pathway components has been connected with essential tumor properties, such as tumor cell proliferation, antiapoptotic properties, invasive behavior and chemotherapy resistance. However, the effects of photodynamic therapy (PDT), one of the cancer treatment methods for the regulation of the IGF signaling pathway, are still unclear. The aim of this study was to investigate the expression of IGF-2 after 5-aminolevulinic acid (5-ALA)-mediated-PDT in SW620 human colorectal cancer cells with evaluation of cell proliferation and apoptosis and to determine the effects of PDT on the IGF-2 receptor (IGF-2R), IGF-2 binding protein-1 (IGF-2BP-1) and the proapoptotic protein, BAX. Cells were treated with 5-aminolevulinic acid and its methyl ester. Changes of the expression and concentration of IGF-2 before and after treatment were assayed by immunocytochemistry, Western blot and ELISA. We found that IGF-2 was significantly overexpressed in the SW620 cell line, while its receptor and binding protein-1 were not significantly changed. Within this study, we would like to suggest that IGF-2 contributes to the effects of PDT and that its expression will influence post-PDT efficacy. |
| first_indexed | 2024-04-12T02:12:38Z |
| format | Article |
| id | doaj.art-87b030bf1aa940948d0c58e79da085c8 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-12T02:12:38Z |
| publishDate | 2015-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-87b030bf1aa940948d0c58e79da085c82022-12-22T03:52:20ZengMDPI AGInternational Journal of Molecular Sciences1422-00672015-10-011610236152362910.3390/ijms161023615ijms161023615Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell LineMarta Woźniak0Kamila Duś-Szachniewicz1Piotr Ziółkowski2Department of Pathology, Wroclaw Medical University, Wrocław 50-368, PolandDepartment of Pathology, Wroclaw Medical University, Wrocław 50-368, PolandDepartment of Pathology, Wroclaw Medical University, Wrocław 50-368, PolandThe IGF system is a family of polypeptide growth factors, which plays a significant role in the development and growth of many cells. Dysregulation of insulin-like growth factors and their pathway components has been connected with essential tumor properties, such as tumor cell proliferation, antiapoptotic properties, invasive behavior and chemotherapy resistance. However, the effects of photodynamic therapy (PDT), one of the cancer treatment methods for the regulation of the IGF signaling pathway, are still unclear. The aim of this study was to investigate the expression of IGF-2 after 5-aminolevulinic acid (5-ALA)-mediated-PDT in SW620 human colorectal cancer cells with evaluation of cell proliferation and apoptosis and to determine the effects of PDT on the IGF-2 receptor (IGF-2R), IGF-2 binding protein-1 (IGF-2BP-1) and the proapoptotic protein, BAX. Cells were treated with 5-aminolevulinic acid and its methyl ester. Changes of the expression and concentration of IGF-2 before and after treatment were assayed by immunocytochemistry, Western blot and ELISA. We found that IGF-2 was significantly overexpressed in the SW620 cell line, while its receptor and binding protein-1 were not significantly changed. Within this study, we would like to suggest that IGF-2 contributes to the effects of PDT and that its expression will influence post-PDT efficacy.http://www.mdpi.com/1422-0067/16/10/23615photodynamic therapyinsulin-like growth factor-2insulin-like growth factor-2 receptorinsulin-like growth factor-2 binding protein-1 |
| spellingShingle | Marta Woźniak Kamila Duś-Szachniewicz Piotr Ziółkowski Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line International Journal of Molecular Sciences photodynamic therapy insulin-like growth factor-2 insulin-like growth factor-2 receptor insulin-like growth factor-2 binding protein-1 |
| title | Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line |
| title_full | Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line |
| title_fullStr | Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line |
| title_full_unstemmed | Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line |
| title_short | Insulin-Like Growth Factor-2 Is Induced Following 5-Aminolevulinic Acid-Mediated Photodynamic Therapy in SW620 Human Colon Cancer Cell Line |
| title_sort | insulin like growth factor 2 is induced following 5 aminolevulinic acid mediated photodynamic therapy in sw620 human colon cancer cell line |
| topic | photodynamic therapy insulin-like growth factor-2 insulin-like growth factor-2 receptor insulin-like growth factor-2 binding protein-1 |
| url | http://www.mdpi.com/1422-0067/16/10/23615 |
| work_keys_str_mv | AT martawozniak insulinlikegrowthfactor2isinducedfollowing5aminolevulinicacidmediatedphotodynamictherapyinsw620humancoloncancercellline AT kamiladusszachniewicz insulinlikegrowthfactor2isinducedfollowing5aminolevulinicacidmediatedphotodynamictherapyinsw620humancoloncancercellline AT piotrziołkowski insulinlikegrowthfactor2isinducedfollowing5aminolevulinicacidmediatedphotodynamictherapyinsw620humancoloncancercellline |