Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP)
(1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimic...
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2076-0817/11/10/1082 |
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| author | Leresche Even Doneilly Oyaba Yinda Richard Onanga Pierre Philippe Mbehang Nguema Etienne François Akomo-Okoue Gontran Nsi Akoue Neil Michel Longo Pendy Desire Otsaghe Ekore Roméo Wenceslas Lendamba Arsène Mabika-Mabika Jean Constant Obague Mbeang Natacha Poungou Ibrahim Jacques François Mavoungou Sylvain Godreuil |
| author_facet | Leresche Even Doneilly Oyaba Yinda Richard Onanga Pierre Philippe Mbehang Nguema Etienne François Akomo-Okoue Gontran Nsi Akoue Neil Michel Longo Pendy Desire Otsaghe Ekore Roméo Wenceslas Lendamba Arsène Mabika-Mabika Jean Constant Obague Mbeang Natacha Poungou Ibrahim Jacques François Mavoungou Sylvain Godreuil |
| author_sort | Leresche Even Doneilly Oyaba Yinda |
| collection | DOAJ |
| description | (1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimicrobial resistance of <i>Escherichia coli</i> isolated from western lowland gorilla’s faeces living in MDNP. (2) Materials and Methods: Ninety-six faecal samples were collected from western lowland gorillas (<i>Gorilla gorilla gorilla</i>) during daily monitoring in the MDNP. Sixty-four <i>E. coli</i> isolates were obtained and screened for phylogenetic and pathotype group genes by polymerase chain reaction (PCR) after DNA extraction. In addition, antimicrobial susceptibility was determined by the disk diffusion method on Mueller Hinton agar. (3) Results: Sixty-four (64%) isolates of <i>E. coli</i> were obtained from samples. A high level of resistance to the beta-lactam family, a moderate rate for fluoroquinolone and a low rate for aminoglycoside was obtained. All <i>E. coli</i> isolates were positive in phylogroup PCR with a predominance of A (69% ± 11.36%), followed by B2 (20% ± 19.89%) and B1 (10% ± 8.90%) and low prevalence for D (1% ± 3.04%). In addition, twenty <i>E. coli</i> isolates (31%) were positive for pathotype PCR, such as EPEC (85% ± 10.82%) and EPEC/EHEC (15% ± 5.18%) that were obtained in this study. The majority of these MDR <i>E. coli</i> (DECs) belonged to phylogenetic group A, followed by MDR <i>E. coli</i> (DECs) belonging to group B2. (4) Conclusion: This study is the first description of MDR <i>E. coli</i> (DECs) assigned to phylogroup A in western lowland gorillas from the MDNP in Gabon. Thus, wild gorillas in MDNP could be considered as asymptomatic carriers of potential pathogenic MDR <i>E. coli</i> (DECs) that may present a potential risk to human health. |
| first_indexed | 2024-03-09T19:38:18Z |
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| id | doaj.art-87b0870552464c17817a21a98b8d9b84 |
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| language | English |
| last_indexed | 2024-03-09T19:38:18Z |
| publishDate | 2022-09-01 |
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| series | Pathogens |
| spelling | doaj.art-87b0870552464c17817a21a98b8d9b842023-11-24T01:47:18ZengMDPI AGPathogens2076-08172022-09-011110108210.3390/pathogens11101082Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP)Leresche Even Doneilly Oyaba Yinda0Richard Onanga1Pierre Philippe Mbehang Nguema2Etienne François Akomo-Okoue3Gontran Nsi Akoue4Neil Michel Longo Pendy5Desire Otsaghe Ekore6Roméo Wenceslas Lendamba7Arsène Mabika-Mabika8Jean Constant Obague Mbeang9Natacha Poungou10Ibrahim11Jacques François Mavoungou12Sylvain Godreuil13Laboratory of Bacteriology, Interdisciplinary Medical Research Center of Franceville, Franceville P.O. Box 769, GabonLaboratory of Bacteriology, Interdisciplinary Medical Research Center of Franceville, Franceville P.O. Box 769, GabonMicrobiology Laboratory, Research Institute for Tropical Ecology, Libreville P.O. Box 13354, GabonMicrobiology Laboratory, Research Institute for Tropical Ecology, Libreville P.O. Box 13354, GabonÉcole Normale Supérieure (ENS), Libreville P.O. Box 17009, GabonLaboratory of Vector Ecology, Interdisciplinary Medical Research Center of Franceville, Franceville P.O. Box 769, GabonLaboratory of Bacteriology, Interdisciplinary Medical Research Center of Franceville, Franceville P.O. Box 769, GabonLaboratory of Bacteriology, Interdisciplinary Medical Research Center of Franceville, Franceville P.O. Box 769, GabonLaboratory of Bacteriology, Interdisciplinary Medical Research Center of Franceville, Franceville P.O. Box 769, GabonMicrobiology Laboratory, Research Institute for Tropical Ecology, Libreville P.O. Box 13354, GabonMicrobiology Laboratory, Research Institute for Tropical Ecology, Libreville P.O. Box 13354, GabonLaboratory of Biology, University of Science and Technology of Masuku, Franceville P.O. Box 913, GabonMicrobiology Laboratory, Research Institute for Tropical Ecology, Libreville P.O. Box 13354, GabonLaboratoire de Bactériologie, CHU de Montpellier, UMR MIVEGEC (IRD, CNRS, Université de Montpellier), 34295 Montpellier, France(1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimicrobial resistance of <i>Escherichia coli</i> isolated from western lowland gorilla’s faeces living in MDNP. (2) Materials and Methods: Ninety-six faecal samples were collected from western lowland gorillas (<i>Gorilla gorilla gorilla</i>) during daily monitoring in the MDNP. Sixty-four <i>E. coli</i> isolates were obtained and screened for phylogenetic and pathotype group genes by polymerase chain reaction (PCR) after DNA extraction. In addition, antimicrobial susceptibility was determined by the disk diffusion method on Mueller Hinton agar. (3) Results: Sixty-four (64%) isolates of <i>E. coli</i> were obtained from samples. A high level of resistance to the beta-lactam family, a moderate rate for fluoroquinolone and a low rate for aminoglycoside was obtained. All <i>E. coli</i> isolates were positive in phylogroup PCR with a predominance of A (69% ± 11.36%), followed by B2 (20% ± 19.89%) and B1 (10% ± 8.90%) and low prevalence for D (1% ± 3.04%). In addition, twenty <i>E. coli</i> isolates (31%) were positive for pathotype PCR, such as EPEC (85% ± 10.82%) and EPEC/EHEC (15% ± 5.18%) that were obtained in this study. The majority of these MDR <i>E. coli</i> (DECs) belonged to phylogenetic group A, followed by MDR <i>E. coli</i> (DECs) belonging to group B2. (4) Conclusion: This study is the first description of MDR <i>E. coli</i> (DECs) assigned to phylogroup A in western lowland gorillas from the MDNP in Gabon. Thus, wild gorillas in MDNP could be considered as asymptomatic carriers of potential pathogenic MDR <i>E. coli</i> (DECs) that may present a potential risk to human health.https://www.mdpi.com/2076-0817/11/10/1082<i>E. coli</i>antimicrobial resistancephylogenetic grouppathotypegorillaMDNP |
| spellingShingle | Leresche Even Doneilly Oyaba Yinda Richard Onanga Pierre Philippe Mbehang Nguema Etienne François Akomo-Okoue Gontran Nsi Akoue Neil Michel Longo Pendy Desire Otsaghe Ekore Roméo Wenceslas Lendamba Arsène Mabika-Mabika Jean Constant Obague Mbeang Natacha Poungou Ibrahim Jacques François Mavoungou Sylvain Godreuil Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP) Pathogens <i>E. coli</i> antimicrobial resistance phylogenetic group pathotype gorilla MDNP |
| title | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP) |
| title_full | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP) |
| title_fullStr | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP) |
| title_full_unstemmed | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP) |
| title_short | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of <i>Escherichia coli</i> Isolated from Western Lowland Gorilla Faeces (<i>Gorilla gorilla gorilla</i>) of Moukalaba-Doudou National Park (MDNP) |
| title_sort | phylogenetic groups pathotypes and antimicrobial resistance of i escherichia coli i isolated from western lowland gorilla faeces i gorilla gorilla gorilla i of moukalaba doudou national park mdnp |
| topic | <i>E. coli</i> antimicrobial resistance phylogenetic group pathotype gorilla MDNP |
| url | https://www.mdpi.com/2076-0817/11/10/1082 |
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