MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE
This study aimed to evaluate the in vitro efficacy of commercial disinfectants on Staphylococcus spp., isolated from mastitis cases in goats, and to associate the observed resistance with the presence of the icaD gene. Broth microdilution was employed to evaluate the in vitro antimicrobial activity...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Goiás
2019-07-01
|
| Series: | Ciência Animal Brasileira |
| Online Access: | https://www.revistas.ufg.br/vet/article/view/53193 |
| _version_ | 1819021250168619008 |
|---|---|
| author | Welington Erasmo de Lima e Silva Evandro Santos Amanso Rodolfo de Moraes Peixoto João José de Simoni Gouveia Gisele Veneroni Gouveia Mateus Matiuzzi da Costa |
| author_facet | Welington Erasmo de Lima e Silva Evandro Santos Amanso Rodolfo de Moraes Peixoto João José de Simoni Gouveia Gisele Veneroni Gouveia Mateus Matiuzzi da Costa |
| author_sort | Welington Erasmo de Lima e Silva |
| collection | DOAJ |
| description | This study aimed to evaluate the in vitro efficacy of commercial disinfectants on Staphylococcus spp., isolated from mastitis cases in goats, and to associate the observed resistance with the presence of the icaD gene. Broth microdilution was employed to evaluate the in vitro antimicrobial activity of the disinfectants, whereas the Congo Red technique was used for the evaluation of biofilm production and amplification of the icaD gene. All evaluated samples were sensitive to disinfectants, with the following ranges of activity: quaternary ammonium (0.13 – 21.33 µg/ml), chlorhexidine (4.00 – 313.00 µg/ml) and iodine (190.00 – 12500.00 µg/ml); however, the sodium hypochlorite-based disinfectant showed no bactericidal activity in the concentration range from 15.0 to 0.03 μg/ml. The icaD gene presented a frequency of 14.7% in the isolate samples. Fisher's exact test showed a significant effect of the relation between the minimum bactericidal concentration value of the quaternary ammonium-based disinfectant and the presence/absence of the icaD gene (P <0.01). Commercial disinfectants with quaternary ammonium, chlorhexidine and iodine active ingredients presented in vitro activity even at concentrations lower than those recommended by the manufacturers. Therefore, the periodic evaluation of the sensitivity profile of the disinfectants must be performed.
Keywords: antimicrobial activity; biofilm; dairy goat; milking; chemical substances
Resumo |
| first_indexed | 2024-12-21T04:04:07Z |
| format | Article |
| id | doaj.art-87b542cb1a024f08b39b38f3a9615708 |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-12-21T04:04:07Z |
| publishDate | 2019-07-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-87b542cb1a024f08b39b38f3a96157082022-12-21T19:16:39ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912019-07-01201853193MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENEWelington Erasmo de Lima e SilvaEvandro Santos AmansoRodolfo de Moraes PeixotoJoão José de Simoni GouveiaGisele Veneroni GouveiaMateus Matiuzzi da CostaThis study aimed to evaluate the in vitro efficacy of commercial disinfectants on Staphylococcus spp., isolated from mastitis cases in goats, and to associate the observed resistance with the presence of the icaD gene. Broth microdilution was employed to evaluate the in vitro antimicrobial activity of the disinfectants, whereas the Congo Red technique was used for the evaluation of biofilm production and amplification of the icaD gene. All evaluated samples were sensitive to disinfectants, with the following ranges of activity: quaternary ammonium (0.13 – 21.33 µg/ml), chlorhexidine (4.00 – 313.00 µg/ml) and iodine (190.00 – 12500.00 µg/ml); however, the sodium hypochlorite-based disinfectant showed no bactericidal activity in the concentration range from 15.0 to 0.03 μg/ml. The icaD gene presented a frequency of 14.7% in the isolate samples. Fisher's exact test showed a significant effect of the relation between the minimum bactericidal concentration value of the quaternary ammonium-based disinfectant and the presence/absence of the icaD gene (P <0.01). Commercial disinfectants with quaternary ammonium, chlorhexidine and iodine active ingredients presented in vitro activity even at concentrations lower than those recommended by the manufacturers. Therefore, the periodic evaluation of the sensitivity profile of the disinfectants must be performed. Keywords: antimicrobial activity; biofilm; dairy goat; milking; chemical substances Resumohttps://www.revistas.ufg.br/vet/article/view/53193 |
| spellingShingle | Welington Erasmo de Lima e Silva Evandro Santos Amanso Rodolfo de Moraes Peixoto João José de Simoni Gouveia Gisele Veneroni Gouveia Mateus Matiuzzi da Costa MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE Ciência Animal Brasileira |
| title | MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE |
| title_full | MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE |
| title_fullStr | MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE |
| title_full_unstemmed | MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE |
| title_short | MINIMUM BACTERICIDAL CONCENTRATION OF COMMERCIAL DISINFECTANTS ON Staphylococcus SPP. ISOLATED FROM MASTITIS IN GOATS AND DETECTION OF THE icaD GENE |
| title_sort | minimum bactericidal concentration of commercial disinfectants on staphylococcus spp isolated from mastitis in goats and detection of the icad gene |
| url | https://www.revistas.ufg.br/vet/article/view/53193 |
| work_keys_str_mv | AT welingtonerasmodelimaesilva minimumbactericidalconcentrationofcommercialdisinfectantsonstaphylococcussppisolatedfrommastitisingoatsanddetectionoftheicadgene AT evandrosantosamanso minimumbactericidalconcentrationofcommercialdisinfectantsonstaphylococcussppisolatedfrommastitisingoatsanddetectionoftheicadgene AT rodolfodemoraespeixoto minimumbactericidalconcentrationofcommercialdisinfectantsonstaphylococcussppisolatedfrommastitisingoatsanddetectionoftheicadgene AT joaojosedesimonigouveia minimumbactericidalconcentrationofcommercialdisinfectantsonstaphylococcussppisolatedfrommastitisingoatsanddetectionoftheicadgene AT giseleveneronigouveia minimumbactericidalconcentrationofcommercialdisinfectantsonstaphylococcussppisolatedfrommastitisingoatsanddetectionoftheicadgene AT mateusmatiuzzidacosta minimumbactericidalconcentrationofcommercialdisinfectantsonstaphylococcussppisolatedfrommastitisingoatsanddetectionoftheicadgene |