Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China
Tropical Asian collections of Inosperma are usually poisonous mushrooms that have caused many poisoning incidents. However, the species diversity and the toxic mechanisms of these Inosperma species are still unclear. In this study, we describe the discovery of Inosperma wuzhishanense sp. nov. from W...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1326253/full |
| _version_ | 1797401359708848128 |
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| author | Yu-Ling Zhou Lun-Sha Deng Shu-Dong Yang Chao-Feng Liu Yu-Guang Fan Wen-Jie Yu |
| author_facet | Yu-Ling Zhou Lun-Sha Deng Shu-Dong Yang Chao-Feng Liu Yu-Guang Fan Wen-Jie Yu |
| author_sort | Yu-Ling Zhou |
| collection | DOAJ |
| description | Tropical Asian collections of Inosperma are usually poisonous mushrooms that have caused many poisoning incidents. However, the species diversity and the toxic mechanisms of these Inosperma species are still unclear. In this study, we describe the discovery of Inosperma wuzhishanense sp. nov. from Wuzhishan City, Hainan Province, tropical China. The new species was identified based on morphological and multi-locus (ITS, nrLSU, and RPB2) phylogenetic analyses. The new species is characterized by its reddish-brown pileus, fibrillose stipes with finely protruding fibrils, rather crowded lamellae, smooth and ellipsoid basidiospores, and mostly clavate, thin-walled cheilocystidia. The new species is phylogenetically nested in the Old World tropical clade 2 and is sister to the tropical Indian taxa I. akirnum. Detailed descriptions, color photos of the new species, and comparisons with its closely related species are provided. Additionally, the muscarine content of the new species was analyzed by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC–MS/MS). The muscarine contents ranged from 4,359.79 ± 83.87 mg/kg to 7,114.03 ± 76.55 mg/kg, 2,748.37 ± 106.85 mg/kg to 4,491.35 ± 467.21 mg/kg, and 2,301.36 ± 83.52 mg/kg to 2,775.90 ± 205.624 mg/kg in the stipe, pileus, and lamellae, respectively. The elemental composition and concentration were determined using inductively coupled plasma-mass spectrometry (ICP-MS). A total of 24 elements were detected. Among the heavy metals detected, arsenic showed the highest level of toxicity with a concentration of 36.76 ± 0.43 mg/kg. |
| first_indexed | 2024-03-09T02:08:55Z |
| format | Article |
| id | doaj.art-87b9c9b81def452bacb5a473939464df |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-09T02:08:55Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-87b9c9b81def452bacb5a473939464df2023-12-07T15:13:21ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-12-011410.3389/fmicb.2023.13262531326253Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical ChinaYu-Ling Zhou0Lun-Sha Deng1Shu-Dong Yang2Chao-Feng Liu3Yu-Guang Fan4Wen-Jie Yu5Hainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Haikou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for R & D of Tropical Herbs, Tropical Environment and Health Laboratory, School of Pharmacy & International School of Public Health and One Health, Hainan Medical University, Haikou, Hainan, ChinaChangchun Food and Drug Inspection Center, Changchun, Jilin, ChinaHainan Institute for Food Control, Key Laboratory of Tropical Fruits and Vegetables Quality and Safety for State Market Regulation, Haikou, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for R & D of Tropical Herbs, Tropical Environment and Health Laboratory, School of Pharmacy & International School of Public Health and One Health, Hainan Medical University, Haikou, Hainan, ChinaKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Key Laboratory for R & D of Tropical Herbs, Tropical Environment and Health Laboratory, School of Pharmacy & International School of Public Health and One Health, Hainan Medical University, Haikou, Hainan, ChinaTropical Asian collections of Inosperma are usually poisonous mushrooms that have caused many poisoning incidents. However, the species diversity and the toxic mechanisms of these Inosperma species are still unclear. In this study, we describe the discovery of Inosperma wuzhishanense sp. nov. from Wuzhishan City, Hainan Province, tropical China. The new species was identified based on morphological and multi-locus (ITS, nrLSU, and RPB2) phylogenetic analyses. The new species is characterized by its reddish-brown pileus, fibrillose stipes with finely protruding fibrils, rather crowded lamellae, smooth and ellipsoid basidiospores, and mostly clavate, thin-walled cheilocystidia. The new species is phylogenetically nested in the Old World tropical clade 2 and is sister to the tropical Indian taxa I. akirnum. Detailed descriptions, color photos of the new species, and comparisons with its closely related species are provided. Additionally, the muscarine content of the new species was analyzed by ultra-performance liquid chromatography/tandem mass spectrometry (UPLC–MS/MS). The muscarine contents ranged from 4,359.79 ± 83.87 mg/kg to 7,114.03 ± 76.55 mg/kg, 2,748.37 ± 106.85 mg/kg to 4,491.35 ± 467.21 mg/kg, and 2,301.36 ± 83.52 mg/kg to 2,775.90 ± 205.624 mg/kg in the stipe, pileus, and lamellae, respectively. The elemental composition and concentration were determined using inductively coupled plasma-mass spectrometry (ICP-MS). A total of 24 elements were detected. Among the heavy metals detected, arsenic showed the highest level of toxicity with a concentration of 36.76 ± 0.43 mg/kg.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1326253/fullnew speciestropical ChinaOld World tropical clade 2muscarineelement |
| spellingShingle | Yu-Ling Zhou Lun-Sha Deng Shu-Dong Yang Chao-Feng Liu Yu-Guang Fan Wen-Jie Yu Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China Frontiers in Microbiology new species tropical China Old World tropical clade 2 muscarine element |
| title | Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China |
| title_full | Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China |
| title_fullStr | Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China |
| title_full_unstemmed | Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China |
| title_short | Phylogenetic analysis, morphological studies, element profiling, and muscarine detection reveal a new toxic Inosperma (Inocybaceae, Agaricales) species from tropical China |
| title_sort | phylogenetic analysis morphological studies element profiling and muscarine detection reveal a new toxic inosperma inocybaceae agaricales species from tropical china |
| topic | new species tropical China Old World tropical clade 2 muscarine element |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1326253/full |
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