Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways.
Allostery is conformation regulation by propagating a signal from one site to another distal site. This study focuses on the long-range communication in DNA mismatch repair proteins MutS and its homologs where intramolecular signaling has to travel over 70 Å to couple lesion detection to ATPase acti...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-10-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC5074593?pdf=render |
| _version_ | 1818024237856718848 |
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| author | Beibei Wang Joshua Francis Monika Sharma Sean M Law Alexander V Predeus Michael Feig |
| author_facet | Beibei Wang Joshua Francis Monika Sharma Sean M Law Alexander V Predeus Michael Feig |
| author_sort | Beibei Wang |
| collection | DOAJ |
| description | Allostery is conformation regulation by propagating a signal from one site to another distal site. This study focuses on the long-range communication in DNA mismatch repair proteins MutS and its homologs where intramolecular signaling has to travel over 70 Å to couple lesion detection to ATPase activity and eventual downstream repair. Using dynamic network analysis based on extensive molecular dynamics simulations, multiple preserved communication pathways were identified that would allow such long-range signaling. The pathways appear to depend on the nucleotides bound to the ATPase domain as well as the type of DNA substrate consistent with previously proposed functional cycles of mismatch recognition and repair initiation by MutS and homologs. A mechanism is proposed where pathways are switched without major conformational rearrangements allowing for efficient long-range signaling and allostery. |
| first_indexed | 2024-12-10T03:57:02Z |
| format | Article |
| id | doaj.art-87ba5896722845cc917f25197977e4d1 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-10T03:57:02Z |
| publishDate | 2016-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-87ba5896722845cc917f25197977e4d12022-12-22T02:03:06ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582016-10-011210e100515910.1371/journal.pcbi.1005159Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways.Beibei WangJoshua FrancisMonika SharmaSean M LawAlexander V PredeusMichael FeigAllostery is conformation regulation by propagating a signal from one site to another distal site. This study focuses on the long-range communication in DNA mismatch repair proteins MutS and its homologs where intramolecular signaling has to travel over 70 Å to couple lesion detection to ATPase activity and eventual downstream repair. Using dynamic network analysis based on extensive molecular dynamics simulations, multiple preserved communication pathways were identified that would allow such long-range signaling. The pathways appear to depend on the nucleotides bound to the ATPase domain as well as the type of DNA substrate consistent with previously proposed functional cycles of mismatch recognition and repair initiation by MutS and homologs. A mechanism is proposed where pathways are switched without major conformational rearrangements allowing for efficient long-range signaling and allostery.http://europepmc.org/articles/PMC5074593?pdf=render |
| spellingShingle | Beibei Wang Joshua Francis Monika Sharma Sean M Law Alexander V Predeus Michael Feig Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways. PLoS Computational Biology |
| title | Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways. |
| title_full | Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways. |
| title_fullStr | Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways. |
| title_full_unstemmed | Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways. |
| title_short | Long-Range Signaling in MutS and MSH Homologs via Switching of Dynamic Communication Pathways. |
| title_sort | long range signaling in muts and msh homologs via switching of dynamic communication pathways |
| url | http://europepmc.org/articles/PMC5074593?pdf=render |
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