The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palm
In the United Arab Emirates (UAE), sudden decline syndrome (SDS) is one of the major fungal diseases caused by Fusarium solani affecting date palm plantations. To minimize the impact of the causal agent of SDS on date palm, native actinobacterial strains isolated from rhizosphere soils of healthy da...
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| Format: | Article |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.904166/full |
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| author | Khawla J. Alwahshi Khawla J. Alwahshi Gouthaman P. Purayil Esam Eldin Saeed Haneen A. Abufarajallah Shama J. Aldhaheri Synan F. AbuQamar Khaled A. El-Tarabily Khaled A. El-Tarabily Khaled A. El-Tarabily |
| author_facet | Khawla J. Alwahshi Khawla J. Alwahshi Gouthaman P. Purayil Esam Eldin Saeed Haneen A. Abufarajallah Shama J. Aldhaheri Synan F. AbuQamar Khaled A. El-Tarabily Khaled A. El-Tarabily Khaled A. El-Tarabily |
| author_sort | Khawla J. Alwahshi |
| collection | DOAJ |
| description | In the United Arab Emirates (UAE), sudden decline syndrome (SDS) is one of the major fungal diseases caused by Fusarium solani affecting date palm plantations. To minimize the impact of the causal agent of SDS on date palm, native actinobacterial strains isolated from rhizosphere soils of healthy date palm plants were characterized according to their antifungal activities against F. solani DSM 106836 (Fs). Based on their in vitro abilities, two promising biocontrol agents (BCAs), namely Streptomyces tendae UAE1 (St) andStreptomyces violaceoruber UAE1 (Sv), were selected for the production of antifungal compounds and cell wall degrading enzymes (CWDEs), albeit their variations in synthesizing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCD). Although both isolates showed antagonism when applied 7 days before the pathogen in the greenhouse experiments, the ACCD-producing Sv was relatively superior in its efficacy against SDS over the non-ACCD-producing St. This was evident from the symptoms of SDS in diseased date palm seedlings which were greatly reduced by Sv compared to St. On a scale of 5.0, the estimated disease severity indices in Fs-diseased seedlings were significantly (P < 0.05) reduced from 4.8 to 1.5 and 0.5 by St and Sv, respectively. Thus, the number of conidia of Fs recovered from plants pre-treated with both BCAs was comparable, but significantly (P < 0.05) reduced compared to plants without any BCA treatment. In addition, a significant (P < 0.05) decrease in ACC levels of both the root and shoot tissues was detected inSv + Fs seedlings to almost similar levels of healthy seedlings. However, in planta ACC levels highly increased in seedlings grown in soils infested with the pathogen alone or amended with St prior to F. solani infestation (St + Fs). This suggests a major role of ACCD production in relieving the stress of date palm seedlings infected with F. solani, thus supporting the integrated preventive disease management programs against this pathogen. This is the first report of effective rhizosphere actinobacterial BCAs to provide protection against SDS on date palm, and to help increase agricultural productivity in a more sustainable manner in the UAE and the other arid regions. |
| first_indexed | 2024-04-12T08:29:43Z |
| format | Article |
| id | doaj.art-87c285fc45024c4a86eee75f1e9e2f85 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T08:29:43Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-87c285fc45024c4a86eee75f1e9e2f852022-12-22T03:40:14ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-07-011310.3389/fpls.2022.904166904166The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palmKhawla J. Alwahshi0Khawla J. Alwahshi1Gouthaman P. Purayil2Esam Eldin Saeed3Haneen A. Abufarajallah4Shama J. Aldhaheri5Synan F. AbuQamar6Khaled A. El-Tarabily7Khaled A. El-Tarabily8Khaled A. El-Tarabily9Department of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesResearch Station Section, Abu Dhabi Agriculture and Food Safety Authority, Abu Dhabi, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al-Ain, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesDepartment of Biology, College of Science, United Arab Emirates University, Al-Ain, United Arab EmiratesKhalifa Center for Genetic Engineering and Biotechnology, United Arab Emirates University, Al-Ain, United Arab EmiratesHarry Butler Institute, Murdoch University, Murdoch, WA, AustraliaIn the United Arab Emirates (UAE), sudden decline syndrome (SDS) is one of the major fungal diseases caused by Fusarium solani affecting date palm plantations. To minimize the impact of the causal agent of SDS on date palm, native actinobacterial strains isolated from rhizosphere soils of healthy date palm plants were characterized according to their antifungal activities against F. solani DSM 106836 (Fs). Based on their in vitro abilities, two promising biocontrol agents (BCAs), namely Streptomyces tendae UAE1 (St) andStreptomyces violaceoruber UAE1 (Sv), were selected for the production of antifungal compounds and cell wall degrading enzymes (CWDEs), albeit their variations in synthesizing 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase (ACCD). Although both isolates showed antagonism when applied 7 days before the pathogen in the greenhouse experiments, the ACCD-producing Sv was relatively superior in its efficacy against SDS over the non-ACCD-producing St. This was evident from the symptoms of SDS in diseased date palm seedlings which were greatly reduced by Sv compared to St. On a scale of 5.0, the estimated disease severity indices in Fs-diseased seedlings were significantly (P < 0.05) reduced from 4.8 to 1.5 and 0.5 by St and Sv, respectively. Thus, the number of conidia of Fs recovered from plants pre-treated with both BCAs was comparable, but significantly (P < 0.05) reduced compared to plants without any BCA treatment. In addition, a significant (P < 0.05) decrease in ACC levels of both the root and shoot tissues was detected inSv + Fs seedlings to almost similar levels of healthy seedlings. However, in planta ACC levels highly increased in seedlings grown in soils infested with the pathogen alone or amended with St prior to F. solani infestation (St + Fs). This suggests a major role of ACCD production in relieving the stress of date palm seedlings infected with F. solani, thus supporting the integrated preventive disease management programs against this pathogen. This is the first report of effective rhizosphere actinobacterial BCAs to provide protection against SDS on date palm, and to help increase agricultural productivity in a more sustainable manner in the UAE and the other arid regions.https://www.frontiersin.org/articles/10.3389/fpls.2022.904166/fullactinobacteriabiocontroldate palmFusarium solanirhizosphereplant–microbe interaction |
| spellingShingle | Khawla J. Alwahshi Khawla J. Alwahshi Gouthaman P. Purayil Esam Eldin Saeed Haneen A. Abufarajallah Shama J. Aldhaheri Synan F. AbuQamar Khaled A. El-Tarabily Khaled A. El-Tarabily Khaled A. El-Tarabily The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palm Frontiers in Plant Science actinobacteria biocontrol date palm Fusarium solani rhizosphere plant–microbe interaction |
| title | The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palm |
| title_full | The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palm |
| title_fullStr | The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palm |
| title_full_unstemmed | The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palm |
| title_short | The 1-aminocyclopropane-1-carboxylic acid deaminase-producing Streptomyces violaceoruber UAE1 can provide protection from sudden decline syndrome on date palm |
| title_sort | 1 aminocyclopropane 1 carboxylic acid deaminase producing streptomyces violaceoruber uae1 can provide protection from sudden decline syndrome on date palm |
| topic | actinobacteria biocontrol date palm Fusarium solani rhizosphere plant–microbe interaction |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.904166/full |
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