Sanitary status of the Grapevine germplasm collection in Republic of Srpska
In July 2015, 179 grapevine plants belonging to 16 grapevine autochthonous cultivars were assessed for sanitary status using DAS ELISA test for the presence of: Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapev...
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| Format: | Article |
| Language: | English |
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University of Banja Luka - Faculty of Agriculture, Banja Luka
2016-01-01
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| Series: | Agroznanje |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/2233-0070/2016/2233-00701602143D.pdf |
| _version_ | 1797205081142067200 |
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| author | Delić Duška Lolić Biljana Đurić Gordana Jovanović-Cvetković Tatjana |
| author_facet | Delić Duška Lolić Biljana Đurić Gordana Jovanović-Cvetković Tatjana |
| author_sort | Delić Duška |
| collection | DOAJ |
| description | In July 2015, 179 grapevine plants belonging to 16 grapevine autochthonous cultivars were assessed for sanitary status using DAS ELISA test for the presence of: Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine leafroll-associated virus 3 (GLRaV-3). Furthermore, survey for the phytoplasma presence and laboratory analyses using nested-PCR/RFLP assay was conducted at the beginning of September 2015 on grapevine cultivars which were not positive in DAS ELISA test for the presence of the four viruses. Out of 179 tested plants with DAS ELISA test, 146 (81%) were positive for the presence of at least one virus. The most widespread viruses were GFLaV-1 and GFLaV-3 with approximately 80 % of grapevines infected. Nested-PCR/RFLP assay showed that out of 33 tested samples 2 were positive for the presence of phytoplasmas from 16SrXII group. Sanitation of infected grapevine cultivars is needed in near future. |
| first_indexed | 2024-04-24T08:45:27Z |
| format | Article |
| id | doaj.art-87c39f3b0da44a1da0166b1522c1ee51 |
| institution | Directory Open Access Journal |
| issn | 2233-0070 |
| language | English |
| last_indexed | 2024-04-24T08:45:27Z |
| publishDate | 2016-01-01 |
| publisher | University of Banja Luka - Faculty of Agriculture, Banja Luka |
| record_format | Article |
| series | Agroznanje |
| spelling | doaj.art-87c39f3b0da44a1da0166b1522c1ee512024-04-16T13:36:45ZengUniversity of Banja Luka - Faculty of Agriculture, Banja LukaAgroznanje2233-00702016-01-0117214315210.7251/AGREN1602143D2233-00701602143DSanitary status of the Grapevine germplasm collection in Republic of SrpskaDelić Duška0Lolić Biljana1Đurić Gordana2https://orcid.org/0000-0001-6781-1105Jovanović-Cvetković Tatjana3University of Banjaluka, Faculty of Agriculture, Republic of Srpska, Bosnia and HerzegovinaUniversity of Banjaluka, Faculty of Agriculture, Republic of Srpska, Bosnia and HerzegovinaUniversity of Banjaluka, Faculty of Agriculture, Republic of Srpska, Bosnia and HerzegovinaUniversity of Banjaluka, Faculty of Agriculture, Republic of Srpska, Bosnia and HerzegovinaIn July 2015, 179 grapevine plants belonging to 16 grapevine autochthonous cultivars were assessed for sanitary status using DAS ELISA test for the presence of: Grapevine fanleaf virus (GFLV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine leafroll-associated virus 3 (GLRaV-3). Furthermore, survey for the phytoplasma presence and laboratory analyses using nested-PCR/RFLP assay was conducted at the beginning of September 2015 on grapevine cultivars which were not positive in DAS ELISA test for the presence of the four viruses. Out of 179 tested plants with DAS ELISA test, 146 (81%) were positive for the presence of at least one virus. The most widespread viruses were GFLaV-1 and GFLaV-3 with approximately 80 % of grapevines infected. Nested-PCR/RFLP assay showed that out of 33 tested samples 2 were positive for the presence of phytoplasmas from 16SrXII group. Sanitation of infected grapevine cultivars is needed in near future.https://scindeks-clanci.ceon.rs/data/pdf/2233-0070/2016/2233-00701602143D.pdfvitis vinifera lgrapevine fanleaf virusgrapevine leafroll-associated virusphytoplasma |
| spellingShingle | Delić Duška Lolić Biljana Đurić Gordana Jovanović-Cvetković Tatjana Sanitary status of the Grapevine germplasm collection in Republic of Srpska Agroznanje vitis vinifera l grapevine fanleaf virus grapevine leafroll-associated virus phytoplasma |
| title | Sanitary status of the Grapevine germplasm collection in Republic of Srpska |
| title_full | Sanitary status of the Grapevine germplasm collection in Republic of Srpska |
| title_fullStr | Sanitary status of the Grapevine germplasm collection in Republic of Srpska |
| title_full_unstemmed | Sanitary status of the Grapevine germplasm collection in Republic of Srpska |
| title_short | Sanitary status of the Grapevine germplasm collection in Republic of Srpska |
| title_sort | sanitary status of the grapevine germplasm collection in republic of srpska |
| topic | vitis vinifera l grapevine fanleaf virus grapevine leafroll-associated virus phytoplasma |
| url | https://scindeks-clanci.ceon.rs/data/pdf/2233-0070/2016/2233-00701602143D.pdf |
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