Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regeneration
Cartilage has limited self-repair ability, leading to osteoarthritis postinjury and ultimately physical disability in people. In addition, cartilage injury is accompanied by loss of extracellular matrix (ECM) and infiltration of inflammation, which makes cartilage regeneration more difficult. In thi...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127524000285 |
| _version_ | 1797301598505926656 |
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| author | Xi Yuan Guanghao Li Long Huang Meng Zheng Jin Su Junlai Wan Haozhe Cheng Hao Zhu Xulin Jiang Jun Xiao |
| author_facet | Xi Yuan Guanghao Li Long Huang Meng Zheng Jin Su Junlai Wan Haozhe Cheng Hao Zhu Xulin Jiang Jun Xiao |
| author_sort | Xi Yuan |
| collection | DOAJ |
| description | Cartilage has limited self-repair ability, leading to osteoarthritis postinjury and ultimately physical disability in people. In addition, cartilage injury is accompanied by loss of extracellular matrix (ECM) and infiltration of inflammation, which makes cartilage regeneration more difficult. In this study, a hybrid hydrogel of hydroxypropyl chitin and oxidized chondroitin sulfate (HPCH-OCS) with injectable and thermosensitive properties was prepared by a Schiff base reaction. In vitro, the HPCH-OCS hydrogel showed desirable biocompatibility and bioactivity by promoting chondrogenic differentiation of stem cells, maintaining the chondrocyte phenotype, and inhibiting proteins related to cartilage ECM catabolism under inflammatory conditions. In vivo, the HPCH-OCS hydrogel combined with microfracture could effectively repair cartilage defects and promote cartilage regeneration. In conclusion, the HPCH-OCS hydrogel has good biological and mechanical properties, and its combination with microfracture provides an effective strategy for clinical cartilage regeneration. |
| first_indexed | 2024-03-07T23:24:52Z |
| format | Article |
| id | doaj.art-87c6d8e6f6104cc2a5b34ed2047e6767 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-03-07T23:24:52Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-87c6d8e6f6104cc2a5b34ed2047e67672024-02-21T05:23:56ZengElsevierMaterials & Design0264-12752024-02-01238112656Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regenerationXi Yuan0Guanghao Li1Long Huang2Meng Zheng3Jin Su4Junlai Wan5Haozhe Cheng6Hao Zhu7Xulin Jiang8Jun Xiao9Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Orthopedic Hospital, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, ChinaDepartment of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaAnhui University of Science and Technology Institute of Precision Medicine (AUST-IPM), Huainan 232001, China; Research and Clinical Translation Center of Gene Medicine and Tissue Engineering, School of Public Health, Anhui University of Science and Technology, Huainan 232001, China; Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, ChinaDepartment of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaState Key Laboratory of Materials Processing and Die & Mould Technology, School of Materials Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, ChinaDepartment of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, ChinaDepartment of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Corresponding authors.Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, China; Corresponding authors.Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Corresponding authors.Cartilage has limited self-repair ability, leading to osteoarthritis postinjury and ultimately physical disability in people. In addition, cartilage injury is accompanied by loss of extracellular matrix (ECM) and infiltration of inflammation, which makes cartilage regeneration more difficult. In this study, a hybrid hydrogel of hydroxypropyl chitin and oxidized chondroitin sulfate (HPCH-OCS) with injectable and thermosensitive properties was prepared by a Schiff base reaction. In vitro, the HPCH-OCS hydrogel showed desirable biocompatibility and bioactivity by promoting chondrogenic differentiation of stem cells, maintaining the chondrocyte phenotype, and inhibiting proteins related to cartilage ECM catabolism under inflammatory conditions. In vivo, the HPCH-OCS hydrogel combined with microfracture could effectively repair cartilage defects and promote cartilage regeneration. In conclusion, the HPCH-OCS hydrogel has good biological and mechanical properties, and its combination with microfracture provides an effective strategy for clinical cartilage regeneration.http://www.sciencedirect.com/science/article/pii/S0264127524000285Hydroxypropyl chitinChondroitin sulfateMicrofractureCartilage regeneration |
| spellingShingle | Xi Yuan Guanghao Li Long Huang Meng Zheng Jin Su Junlai Wan Haozhe Cheng Hao Zhu Xulin Jiang Jun Xiao Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regeneration Materials & Design Hydroxypropyl chitin Chondroitin sulfate Microfracture Cartilage regeneration |
| title | Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regeneration |
| title_full | Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regeneration |
| title_fullStr | Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regeneration |
| title_full_unstemmed | Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regeneration |
| title_short | Hydroxypropyl chitin-oxidized chondroitin sulfate double-network hydrogel assists microfracture technique to enhance cartilage regeneration |
| title_sort | hydroxypropyl chitin oxidized chondroitin sulfate double network hydrogel assists microfracture technique to enhance cartilage regeneration |
| topic | Hydroxypropyl chitin Chondroitin sulfate Microfracture Cartilage regeneration |
| url | http://www.sciencedirect.com/science/article/pii/S0264127524000285 |
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