miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4
Introduction STAT4 is a transcriptional regulator that has been reported to have oncogenic activities in various cancers. In our study, the posttranscriptional regulatory effect of miR-200a-3p on STAT4 and the prognostic significance of miR-200a-3p and STAT4 were evaluated in bladder cancer (BCa)....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2019-11-01
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| Series: | Archives of Medical Science |
| Subjects: | |
| Online Access: | https://www.archivesofmedicalscience.com/miR-200a-3p-predicts-prognosis-and-inhibits-bladder-cancer-cell-proliferation-by,112696,0,2.html |
| _version_ | 1797435392390070272 |
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| author | Ming Li Jie Li Chaoyang Ye Weiwu Wu Yi Cheng |
| author_facet | Ming Li Jie Li Chaoyang Ye Weiwu Wu Yi Cheng |
| author_sort | Ming Li |
| collection | DOAJ |
| description | Introduction
STAT4 is a transcriptional regulator that has been reported to have oncogenic activities in various cancers. In our study, the posttranscriptional regulatory effect of miR-200a-3p on STAT4 and the prognostic significance of miR-200a-3p and STAT4 were evaluated in bladder cancer (BCa).
Material and methods
Proliferation and apoptosis of BCa cell lines were monitored using CCK-8 and Annexin V-FITC assays, respectively. Gene and protein expression levels in BCa tissues and cells were detected using RT-qPCR and western blotting, respectively.
Results
Significant downregulation of miR-200a-3p and upregulation of STAT4 were observed in BCa tissues and cells compared with the corresponding non-tumor adjacent tissues. Both STAT4 and miR-200a-3p were validated as independent prognostic indicators in sixty-nine BCa patients for predicting overall survival and disease-free survival. In vitro experimental analyses revealed that knockdown of STAT4 repressed BCa cell growth and elevated cell apoptosis. Molecular interactive analysis revealed STAT4 as a direct target of miR-200a-3p, which could suppress STAT4 protein expression by posttranscriptional repression. Cotransfection of miR-200a-3p mimics and STAT4 overexpression plasmids into BCa cells demonstrated that the antineoplastic activities of miR-200a-3p in vitro were neutralized by overexpressed STAT4.
Conclusions
The miR-200a-3p/STAT4 signaling cascade plays an important role in the progression of BCa, which provides a new promising target for targeted BCa therapies. |
| first_indexed | 2024-03-09T10:47:31Z |
| format | Article |
| id | doaj.art-87c72581af574934bf1e3fa51ae27ee9 |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-03-09T10:47:31Z |
| publishDate | 2019-11-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-87c72581af574934bf1e3fa51ae27ee92023-12-01T09:51:47ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512019-11-0119372473510.5114/aoms.2019.89969112696miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4Ming Li0Jie Li1Chaoyang Ye2Weiwu Wu3Yi Cheng4Department of Urology, the Fifth People’s Hospital of Dongguan, Dongguan, Guangdong Province, ChinaDepartment of Urology, the Fifth People’s Hospital of Dongguan, Dongguan, Guangdong Province, ChinaDepartment of Urology, the Fifth People’s Hospital of Dongguan, Dongguan, Guangdong Province, ChinaDepartment of Urology, the Fifth People’s Hospital of Dongguan, Dongguan, Guangdong Province, ChinaDepartment of Urology, the Fifth People’s Hospital of Dongguan, Dongguan, Guangdong Province, ChinaIntroduction STAT4 is a transcriptional regulator that has been reported to have oncogenic activities in various cancers. In our study, the posttranscriptional regulatory effect of miR-200a-3p on STAT4 and the prognostic significance of miR-200a-3p and STAT4 were evaluated in bladder cancer (BCa). Material and methods Proliferation and apoptosis of BCa cell lines were monitored using CCK-8 and Annexin V-FITC assays, respectively. Gene and protein expression levels in BCa tissues and cells were detected using RT-qPCR and western blotting, respectively. Results Significant downregulation of miR-200a-3p and upregulation of STAT4 were observed in BCa tissues and cells compared with the corresponding non-tumor adjacent tissues. Both STAT4 and miR-200a-3p were validated as independent prognostic indicators in sixty-nine BCa patients for predicting overall survival and disease-free survival. In vitro experimental analyses revealed that knockdown of STAT4 repressed BCa cell growth and elevated cell apoptosis. Molecular interactive analysis revealed STAT4 as a direct target of miR-200a-3p, which could suppress STAT4 protein expression by posttranscriptional repression. Cotransfection of miR-200a-3p mimics and STAT4 overexpression plasmids into BCa cells demonstrated that the antineoplastic activities of miR-200a-3p in vitro were neutralized by overexpressed STAT4. Conclusions The miR-200a-3p/STAT4 signaling cascade plays an important role in the progression of BCa, which provides a new promising target for targeted BCa therapies.https://www.archivesofmedicalscience.com/miR-200a-3p-predicts-prognosis-and-inhibits-bladder-cancer-cell-proliferation-by,112696,0,2.htmlbladder cancermir-200a-3pstat4prognosisproliferationposttranscriptional regulation |
| spellingShingle | Ming Li Jie Li Chaoyang Ye Weiwu Wu Yi Cheng miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4 Archives of Medical Science bladder cancer mir-200a-3p stat4 prognosis proliferation posttranscriptional regulation |
| title | miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4 |
| title_full | miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4 |
| title_fullStr | miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4 |
| title_full_unstemmed | miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4 |
| title_short | miR-200a-3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting STAT4 |
| title_sort | mir 200a 3p predicts prognosis and inhibits bladder cancer cell proliferation by targeting stat4 |
| topic | bladder cancer mir-200a-3p stat4 prognosis proliferation posttranscriptional regulation |
| url | https://www.archivesofmedicalscience.com/miR-200a-3p-predicts-prognosis-and-inhibits-bladder-cancer-cell-proliferation-by,112696,0,2.html |
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