Novel KRAS gene mutations in sporadic colorectal cancer.
In this article, we report 7 novel KRAS gene mutations discovered while retrospectively studying the prevalence and pattern of KRAS mutations in cancerous tissue obtained from 56 Saudi sporadic colorectal cancer patients from the Eastern Province.Genomic DNA was extracted from formalin-fixed, paraff...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4239073?pdf=render |
| _version_ | 1818986015199592448 |
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| author | Walid M Naser Mohamed A Shawarby Dalal M Al-Tamimi Arun Seth Abdulaziz Al-Quorain Areej M Al Nemer Omar M E Albagha |
| author_facet | Walid M Naser Mohamed A Shawarby Dalal M Al-Tamimi Arun Seth Abdulaziz Al-Quorain Areej M Al Nemer Omar M E Albagha |
| author_sort | Walid M Naser |
| collection | DOAJ |
| description | In this article, we report 7 novel KRAS gene mutations discovered while retrospectively studying the prevalence and pattern of KRAS mutations in cancerous tissue obtained from 56 Saudi sporadic colorectal cancer patients from the Eastern Province.Genomic DNA was extracted from formalin-fixed, paraffin-embedded cancerous and noncancerous colorectal tissues. Successful and specific PCR products were then bi-directionally sequenced to detect exon 4 mutations while Mutector II Detection Kits were used for identifying mutations in codons 12, 13 and 61. The functional impact of the novel mutations was assessed using bioinformatics tools and molecular modeling.KRAS gene mutations were detected in the cancer tissue of 24 cases (42.85%). Of these, 11 had exon 4 mutations (19.64%). They harbored 8 different mutations all of which except two altered the KRAS protein amino acid sequence and all except one were novel as revealed by COSMIC database. The detected novel mutations were found to be somatic. One mutation is predicted to be benign. The remaining mutations are predicted to cause substantial changes in the protein structure. Of these, the Q150X nonsense mutation is the second truncating mutation to be reported in colorectal cancer in the literature.Our discovery of novel exon 4 KRAS mutations that are, so far, unique to Saudi colorectal cancer patients may be attributed to environmental factors and/or racial/ethnic variations due to genetic differences. Alternatively, it may be related to paucity of clinical studies on mutations other than those in codons 12, 13, 61 and 146. Further KRAS testing on a large number of patients of various ethnicities, particularly beyond the most common hotspot alleles in exons 2 and 3 is needed to assess the prevalence and explore the exact prognostic and predictive significance of the discovered novel mutations as well as their possible role in colorectal carcinogenesis. |
| first_indexed | 2024-12-20T18:44:04Z |
| format | Article |
| id | doaj.art-87c7cdde06b84ff08acd9236768624dd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T18:44:04Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-87c7cdde06b84ff08acd9236768624dd2022-12-21T19:29:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11335010.1371/journal.pone.0113350Novel KRAS gene mutations in sporadic colorectal cancer.Walid M NaserMohamed A ShawarbyDalal M Al-TamimiArun SethAbdulaziz Al-QuorainAreej M Al NemerOmar M E AlbaghaIn this article, we report 7 novel KRAS gene mutations discovered while retrospectively studying the prevalence and pattern of KRAS mutations in cancerous tissue obtained from 56 Saudi sporadic colorectal cancer patients from the Eastern Province.Genomic DNA was extracted from formalin-fixed, paraffin-embedded cancerous and noncancerous colorectal tissues. Successful and specific PCR products were then bi-directionally sequenced to detect exon 4 mutations while Mutector II Detection Kits were used for identifying mutations in codons 12, 13 and 61. The functional impact of the novel mutations was assessed using bioinformatics tools and molecular modeling.KRAS gene mutations were detected in the cancer tissue of 24 cases (42.85%). Of these, 11 had exon 4 mutations (19.64%). They harbored 8 different mutations all of which except two altered the KRAS protein amino acid sequence and all except one were novel as revealed by COSMIC database. The detected novel mutations were found to be somatic. One mutation is predicted to be benign. The remaining mutations are predicted to cause substantial changes in the protein structure. Of these, the Q150X nonsense mutation is the second truncating mutation to be reported in colorectal cancer in the literature.Our discovery of novel exon 4 KRAS mutations that are, so far, unique to Saudi colorectal cancer patients may be attributed to environmental factors and/or racial/ethnic variations due to genetic differences. Alternatively, it may be related to paucity of clinical studies on mutations other than those in codons 12, 13, 61 and 146. Further KRAS testing on a large number of patients of various ethnicities, particularly beyond the most common hotspot alleles in exons 2 and 3 is needed to assess the prevalence and explore the exact prognostic and predictive significance of the discovered novel mutations as well as their possible role in colorectal carcinogenesis.http://europepmc.org/articles/PMC4239073?pdf=render |
| spellingShingle | Walid M Naser Mohamed A Shawarby Dalal M Al-Tamimi Arun Seth Abdulaziz Al-Quorain Areej M Al Nemer Omar M E Albagha Novel KRAS gene mutations in sporadic colorectal cancer. PLoS ONE |
| title | Novel KRAS gene mutations in sporadic colorectal cancer. |
| title_full | Novel KRAS gene mutations in sporadic colorectal cancer. |
| title_fullStr | Novel KRAS gene mutations in sporadic colorectal cancer. |
| title_full_unstemmed | Novel KRAS gene mutations in sporadic colorectal cancer. |
| title_short | Novel KRAS gene mutations in sporadic colorectal cancer. |
| title_sort | novel kras gene mutations in sporadic colorectal cancer |
| url | http://europepmc.org/articles/PMC4239073?pdf=render |
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