Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury
Previous studies have shown that Ninjurin-1 participates in cell trafficking and axonal growth following central and peripheral nervous system neuroinflammation. But its precise roles in these processes and involvement in spinal cord injury pathophysiology remain unclear. Western blot assay revealed...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Neural Regeneration Research |
| Subjects: | |
| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=7;spage=1331;epage=1335;aulast=E. |
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| author | Poornima D E. Weerasinghe-Mudiyanselage Jeongtae Kim Yuna Choi Changjong Moon Taekyun Shin Meejung Ahn |
| author_facet | Poornima D E. Weerasinghe-Mudiyanselage Jeongtae Kim Yuna Choi Changjong Moon Taekyun Shin Meejung Ahn |
| author_sort | Poornima D E. Weerasinghe-Mudiyanselage |
| collection | DOAJ |
| description | Previous studies have shown that Ninjurin-1 participates in cell trafficking and axonal growth following central and peripheral nervous system neuroinflammation. But its precise roles in these processes and involvement in spinal cord injury pathophysiology remain unclear. Western blot assay revealed that Ninjurin-1 levels in rats with spinal cord injury exhibited an upregulation until day 4 post-injury and slightly decreased thereafter compared with sham controls. Immunohistochemistry analysis revealed that Ninjurin-1 immunoreactivity in rats with spinal cord injury sharply increased on days 1 and 4 post-injury and slightly decreased on days 7 and 21 post-injury compared with sham controls. Ninjurin-1 immunostaining was weak in vascular endothelial cells, ependymal cells, and some glial cells in sham controls while it was relatively strong in macrophages, microglia, and reactive astrocytes. These findings suggest that a variety of cells, including vascular endothelial cells, macrophages, and microglia, secrete Ninjurin-1 and they participate in the pathophysiology of compression-induced spinal cord injury. All experimental procedures were approved by the Care and Use of Laboratory Animals of Jeju National University (approval No. 2018-0029) on July 6, 2018. |
| first_indexed | 2024-12-14T12:37:55Z |
| format | Article |
| id | doaj.art-87c956d9cb2e468d87213ec7ed8174f8 |
| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-14T12:37:55Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Neural Regeneration Research |
| spelling | doaj.art-87c956d9cb2e468d87213ec7ed8174f82022-12-21T23:00:59ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742021-01-011671331133510.4103/1673-5374.301033Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injuryPoornima D E. Weerasinghe-MudiyanselageJeongtae KimYuna ChoiChangjong MoonTaekyun ShinMeejung AhnPrevious studies have shown that Ninjurin-1 participates in cell trafficking and axonal growth following central and peripheral nervous system neuroinflammation. But its precise roles in these processes and involvement in spinal cord injury pathophysiology remain unclear. Western blot assay revealed that Ninjurin-1 levels in rats with spinal cord injury exhibited an upregulation until day 4 post-injury and slightly decreased thereafter compared with sham controls. Immunohistochemistry analysis revealed that Ninjurin-1 immunoreactivity in rats with spinal cord injury sharply increased on days 1 and 4 post-injury and slightly decreased on days 7 and 21 post-injury compared with sham controls. Ninjurin-1 immunostaining was weak in vascular endothelial cells, ependymal cells, and some glial cells in sham controls while it was relatively strong in macrophages, microglia, and reactive astrocytes. These findings suggest that a variety of cells, including vascular endothelial cells, macrophages, and microglia, secrete Ninjurin-1 and they participate in the pathophysiology of compression-induced spinal cord injury. All experimental procedures were approved by the Care and Use of Laboratory Animals of Jeju National University (approval No. 2018-0029) on July 6, 2018.http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=7;spage=1331;epage=1335;aulast=E.astrocytes; clip compression injury; macrophage; microglia; neuroinflammation; ninjurin-1; rat; spinal cord |
| spellingShingle | Poornima D E. Weerasinghe-Mudiyanselage Jeongtae Kim Yuna Choi Changjong Moon Taekyun Shin Meejung Ahn Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury Neural Regeneration Research astrocytes; clip compression injury; macrophage; microglia; neuroinflammation; ninjurin-1; rat; spinal cord |
| title | Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury |
| title_full | Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury |
| title_fullStr | Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury |
| title_full_unstemmed | Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury |
| title_short | Ninjurin-1: a biomarker for reflecting the process of neuroinflammation after spinal cord injury |
| title_sort | ninjurin 1 a biomarker for reflecting the process of neuroinflammation after spinal cord injury |
| topic | astrocytes; clip compression injury; macrophage; microglia; neuroinflammation; ninjurin-1; rat; spinal cord |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2021;volume=16;issue=7;spage=1331;epage=1335;aulast=E. |
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