Hedgehog signaling regulates gene expression in planarian glia
Hedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS ma...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/16996 |
| _version_ | 1818019030224601088 |
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| author | Irving E Wang Sylvain W Lapan M Lucila Scimone Thomas R Clandinin Peter W Reddien |
| author_facet | Irving E Wang Sylvain W Lapan M Lucila Scimone Thomas R Clandinin Peter W Reddien |
| author_sort | Irving E Wang |
| collection | DOAJ |
| description | Hedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS maintenance or regeneration. We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched (ptc), which encodes the Hh receptor. Two misregulated genes, intermediate filament-1 (if-1) and calamari (cali), were expressed in a previously unidentified non-neural CNS cell type. These cells expressed orthologs of astrocyte-associated genes involved in neurotransmitter uptake and metabolism, and extended processes enveloping regions of high synapse concentration. We propose that these cells are planarian glia. Planarian glia were distributed broadly, but only expressed if-1 and cali in the neuropil near hh+ neurons. Planarian glia and their regulation by Hedgehog signaling present a novel tractable system for dissection of glia biology. |
| first_indexed | 2024-04-14T07:47:14Z |
| format | Article |
| id | doaj.art-87c98ee316ec432c96a73497b0fcf19e |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:47:14Z |
| publishDate | 2016-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-87c98ee316ec432c96a73497b0fcf19e2022-12-22T02:05:18ZengeLife Sciences Publications LtdeLife2050-084X2016-09-01510.7554/eLife.16996Hedgehog signaling regulates gene expression in planarian gliaIrving E Wang0Sylvain W Lapan1M Lucila Scimone2Thomas R Clandinin3https://orcid.org/0000-0001-6277-6849Peter W Reddien4https://orcid.org/0000-0002-5569-333XDepartment of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States; Whitehead Institute, Massachusetts Institute of Technology, Cambridge, United States; Department of Neurobiology, Stanford University, Stanford, United StatesDepartment of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States; Whitehead Institute, Massachusetts Institute of Technology, Cambridge, United StatesDepartment of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States; Whitehead Institute, Massachusetts Institute of Technology, Cambridge, United StatesDepartment of Neurobiology, Stanford University, Stanford, United StatesDepartment of Biology, Massachusetts Institute of Technology, Cambridge, United States; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States; Whitehead Institute, Massachusetts Institute of Technology, Cambridge, United StatesHedgehog signaling is critical for vertebrate central nervous system (CNS) development, but its role in CNS biology in other organisms is poorly characterized. In the planarian Schmidtea mediterranea, hedgehog (hh) is expressed in medial cephalic ganglia neurons, suggesting a possible role in CNS maintenance or regeneration. We performed RNA sequencing of planarian brain tissue following RNAi of hh and patched (ptc), which encodes the Hh receptor. Two misregulated genes, intermediate filament-1 (if-1) and calamari (cali), were expressed in a previously unidentified non-neural CNS cell type. These cells expressed orthologs of astrocyte-associated genes involved in neurotransmitter uptake and metabolism, and extended processes enveloping regions of high synapse concentration. We propose that these cells are planarian glia. Planarian glia were distributed broadly, but only expressed if-1 and cali in the neuropil near hh+ neurons. Planarian glia and their regulation by Hedgehog signaling present a novel tractable system for dissection of glia biology.https://elifesciences.org/articles/16996planariagliaastrocytesHedgehog signalingregenerationSchmidtea mediterranea |
| spellingShingle | Irving E Wang Sylvain W Lapan M Lucila Scimone Thomas R Clandinin Peter W Reddien Hedgehog signaling regulates gene expression in planarian glia eLife planaria glia astrocytes Hedgehog signaling regeneration Schmidtea mediterranea |
| title | Hedgehog signaling regulates gene expression in planarian glia |
| title_full | Hedgehog signaling regulates gene expression in planarian glia |
| title_fullStr | Hedgehog signaling regulates gene expression in planarian glia |
| title_full_unstemmed | Hedgehog signaling regulates gene expression in planarian glia |
| title_short | Hedgehog signaling regulates gene expression in planarian glia |
| title_sort | hedgehog signaling regulates gene expression in planarian glia |
| topic | planaria glia astrocytes Hedgehog signaling regeneration Schmidtea mediterranea |
| url | https://elifesciences.org/articles/16996 |
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