Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> Serogroup
The aim of the study was the serological and structural characterization of the lipopolysaccharide (LPS) O antigen from <i>P. mirabilis</i> Dm55 coming from the urine of a patient from Lodz. The Dm55 LPS was recognized in ELISA only by the O54 antiserum, suggesting a serological distinct...
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| author | Agata Palusiak Anna Turska-Szewczuk Dominika Drzewiecka |
| author_facet | Agata Palusiak Anna Turska-Szewczuk Dominika Drzewiecka |
| author_sort | Agata Palusiak |
| collection | DOAJ |
| description | The aim of the study was the serological and structural characterization of the lipopolysaccharide (LPS) O antigen from <i>P. mirabilis</i> Dm55 coming from the urine of a patient from Lodz. The Dm55 LPS was recognized in ELISA only by the O54 antiserum, suggesting a serological distinction of the Dm55 O antigen from all the 84 <i>Proteus</i> LPS serotypes described. The obtained polyclonal rabbit serum against <i>P. mirabilis</i> Dm55 reacted in ELISA and Western blotting with a few LPSs (including O54), but the reactions were weaker than those observed in the homologous system. The LPS of <i>P. mirabilis</i> Dm55 was subjected to mild acid hydrolysis, and the obtained high-molecular-mass O polysaccharide was chemically studied using sugar and methylation analyses, mass spectrometry, and <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy, including <sup>1</sup>H,<sup>1</sup>H NOESY, and <sup>1</sup>H,<sup>13</sup>C HMBC experiments. The Dm55 O unit is a branched three-saccharide, and its linear fragment contains α-Gal<i>p</i>NAc and β-Gal<i>p</i>, whereas α-Glc<i>p</i>NAc occupies a terminal position. The Dm55 OPS shares a disaccharide epitope with the <i>Proteus</i> O54 antigen. Due to the structural differences of the studied O antigen from the other described <i>Proteus</i> O polysaccharides, we propose to classify the <i>P. mirabilis</i> Dm55 strain to a new <i>Proteus</i> O85 serogroup. |
| first_indexed | 2024-03-09T16:45:07Z |
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| language | English |
| last_indexed | 2024-03-09T16:45:07Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-87cbcd6755f04d10a1e189de3c9c81242023-11-24T14:47:33ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221642410.3390/ijms242216424Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> SerogroupAgata Palusiak0Anna Turska-Szewczuk1Dominika Drzewiecka2Department of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, PolandDepartment of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, PolandDepartment of Biology of Bacteria, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, PolandThe aim of the study was the serological and structural characterization of the lipopolysaccharide (LPS) O antigen from <i>P. mirabilis</i> Dm55 coming from the urine of a patient from Lodz. The Dm55 LPS was recognized in ELISA only by the O54 antiserum, suggesting a serological distinction of the Dm55 O antigen from all the 84 <i>Proteus</i> LPS serotypes described. The obtained polyclonal rabbit serum against <i>P. mirabilis</i> Dm55 reacted in ELISA and Western blotting with a few LPSs (including O54), but the reactions were weaker than those observed in the homologous system. The LPS of <i>P. mirabilis</i> Dm55 was subjected to mild acid hydrolysis, and the obtained high-molecular-mass O polysaccharide was chemically studied using sugar and methylation analyses, mass spectrometry, and <sup>1</sup>H and <sup>13</sup>C NMR spectroscopy, including <sup>1</sup>H,<sup>1</sup>H NOESY, and <sup>1</sup>H,<sup>13</sup>C HMBC experiments. The Dm55 O unit is a branched three-saccharide, and its linear fragment contains α-Gal<i>p</i>NAc and β-Gal<i>p</i>, whereas α-Glc<i>p</i>NAc occupies a terminal position. The Dm55 OPS shares a disaccharide epitope with the <i>Proteus</i> O54 antigen. Due to the structural differences of the studied O antigen from the other described <i>Proteus</i> O polysaccharides, we propose to classify the <i>P. mirabilis</i> Dm55 strain to a new <i>Proteus</i> O85 serogroup.https://www.mdpi.com/1422-0067/24/22/16424<i>Proteus mirabilis</i>lipopolysaccharidecross-reacting LPSO polysaccharideNMRantiserum |
| spellingShingle | Agata Palusiak Anna Turska-Szewczuk Dominika Drzewiecka Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> Serogroup International Journal of Molecular Sciences <i>Proteus mirabilis</i> lipopolysaccharide cross-reacting LPS O polysaccharide NMR antiserum |
| title | Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> Serogroup |
| title_full | Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> Serogroup |
| title_fullStr | Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> Serogroup |
| title_full_unstemmed | Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> Serogroup |
| title_short | Antigenic and Structural Properties of the Lipopolysaccharide of the Uropathogenic <i>Proteus mirabilis</i> Dm55 Strain Classified to a New O85 <i>Proteus</i> Serogroup |
| title_sort | antigenic and structural properties of the lipopolysaccharide of the uropathogenic i proteus mirabilis i dm55 strain classified to a new o85 i proteus i serogroup |
| topic | <i>Proteus mirabilis</i> lipopolysaccharide cross-reacting LPS O polysaccharide NMR antiserum |
| url | https://www.mdpi.com/1422-0067/24/22/16424 |
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